BBa_K1121000
1
BBa_K1121000
yjgB coding region
2013-09-11T11:00:00Z
2015-05-08T01:09:13Z
This part was amplified by PCR from the New England Biolabs top 10 strain of Escherichia coli.
This medium chain zinc-containing enzyme of the dehydrogenase/reductase family is NADPH-dependent. It has been shown to reduce acetaldehyde and isobutyraldehyde to their corresponding alcohols (http://www.ncbi.nlm.nih.gov/pubmed/22731523).
false
false
_1432_
0
16339
9
In stock
false
No detailed design was done in the creation of this part, other than the design of primers to amplify the gene. The choice of gene was due to its broad-spectrum reduction of aldehydes, which we hoped would allow us to reduce isovaleraldehyde to isoamyl alcohol.
false
Henry Barron
BBa_K1121000_sequence
1
atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgaactggaagtttatgagtacgatcccggtgagctgaggccacaagatgttgaagtgcaggtggattactgcgggatctgccattccgatctgtcgatgatcgataacgaatggggattttcacaatatccgctggttgccgggcatgaggtgattgggcgcgtggtggcactcgggagcgccgcgcaggataaaggtttgcaggtcggtcagcgtgtcgggattggctggacggcgcgtagctgtggtcactgcgacgcctgtattagcggtaatcagatcaactgcgagcaaggtgcggtgccgacgattatgaatcgcggtggctttgccgagaagttgcgtgcggactggcaatgggtgattccactgccagaaaatattgatatcgagtccgccgggccgctgttgtgcggcggtatcacggtctttaaaccactgttgatgcaccatatcactgctaccagccgcgttggggtaattggtattggcgggctggggcatatcgctataaaacttctgcacgcaatgggatgcgaggtgacagcctttagttctaatccggcgaaagagcaggaagtgctggcgatgggtgccgataaagtggtgaatagccgcgatccgcaggcactgaaagcactggcggggcagtttgatctcattatcaacaccgtcaacgtcagcctcgactggcagccctattttgaggcgctgacctatggcggtaatttccatacggtcggtgcggttctcacgccgctgtctgttccggcctttacgttaattgcgggcgatcgcagcgtctctggttctgctaccggcacgccttatgagctgcgtaagctgatgcgttttgccgcccgcagcaaggttgcgccgaccaccgaactgttcccgatgtcgaaaattaacgacgccatccagcatgtgcgcgacggtaaggcgcgttaccgcgtggtgttgaaagccgatttttga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z