BBa_K112121 1 BBa_K112121 BglII endonuclease with ribosome binding site 2008-10-29T12:00:00Z 2015-05-08T01:09:14Z <pre> PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121 </pre> BglII nuclease with ribosome binding site and stop codon false false _224_ 0 3023 9 It's complicated true This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. --- <pre> PCR mv032/mv010 on pBca9145-Bca1147 (722bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112121 ----------------------------------------------- mv032 Forward Biobricking of rbs.r.BglII! cgataGAATTCatgAGATCTtggaaggagacactcataatgaagattgatataacggactataaccatgc mv010 Reverse Biobricking of r.BglII! cgttaGGATCCttaatatgtcacgattgttcctc </pre> false Madhvi Venkatesh BBa_K112121_sequence 1 gatcttggaaggagacactcataatgaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatattaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z