LOCUS BBa_K112246 6768 bp DNA linear 26-Dec-2024 DEFINITION {pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid ACCESSION BBa_K112246 VERSION BBa_K112246.1 FEATURES Location/Qualifiers promoter 893..1769 /label=pAH57 promoter CDS 640..849 /label=ccdB misc_feature 16..140 /label=attR1 CDS 1776..3061 /label=rbs.xis.int! CDS 3068..3386 /label=rbs.ihfA! CDS 3393..3673 /label=rbs.ihfB! misc_feature 3686..3810 /label=attR2 ORIGIN 1 gaattgccat tatcaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata 61 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca 121 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt 181 ccggctcgta taatgtgtgg attttgagtt aggatccggc ttactaaaag ccagataaca 241 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 301 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 361 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 421 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 481 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 541 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 601 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 661 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 721 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 781 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 841 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcagcaat tcgaagattc 901 ttgctcaatt gttatcagct atgcgccgac cagaacacct tgccgatcag ccaaacgtct 961 cttcaggcca ctgactagcg ataactttcc ccacaacgga acaactctca ttgcatggga 1021 tcattgggta ctgtgggttt agtggttgta aaaacacctg accgctatcc ctgatcagtt 1081 tcttgaaggt aaactcatca cccccaagtc tggctatgca gaaatcacct ggctcaacag 1141 cctgctcagg gtcaacgaga attaacattc cgtcaggaaa gcttggcttg gagcctgttg 1201 gtgcggtcat ggaattacct tcaacctcaa gccagaatgc agaatcactg gcttttttgg 1261 ttgtgcttac ccatctctcc gcatcacctt tggtaaaggt tctaagctta ggtgagaaca 1321 tccctgcctg aacatgagaa aaaacagggt actcatactc acttctaagt gacggctgca 1381 tactaaccgc ttcatacatc tcgtagattt ctctggcgat tgaagggcta aattcttcaa 1441 cgctaacttt gagaattttt gtaagcaatg cggcgttata agcatttaat gcattgatgc 1501 cattaaataa agcaccaacg cctgactgcc ccatccccat cttgtctgcg acagattcct 1561 gggataagcc aagttcattt ttcttttttt cataaattgc tttaaggcga cgtgcgtcct 1621 caagctgctc ttgtgttaat ggtttctttt ttgtgctcat acgttaaatc tatcaccgca 1681 agggataaat atctaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt 1741 gcatgtacta aggaggttgt atcaattctg gatcttaatt gcggagactt tgcgatgtac 1801 ttgacacttc aggagtggaa cgcacgccag cgacgtccaa gaagccttga aacagttcgt 1861 cgatgggttc gggaatgcag gatattccca cctccggtta aggatggaag agagtatctg 1921 ttccacgaat cagcggtaaa ggttgactta aatcgaccag taacaggtgg ccttttgaag 1981 aggatcagaa atgggaagaa ggcgaagtca tgagcgccgg gatttacccc ctaaccttta 2041 tataagaaac aatggatatt actgctacag ggacccaagg acgggtaaag agtttggatt 2101 aggcagagac aggcgaatcg caatcactga agctatacag gccaacattg agttattttc 2161 aggacacaaa cacaagcctc tgacagcgag aatcaacagt gataattccg ttacgttaca 2221 ttcatggctt gatcgctacg aaaaaatcct ggccagcaga ggaatcaagc agaagacact 2281 cataaattac atgagcaaaa ttaaagcaat aaggaggggt ctgcctgatg ctccacttga 2341 agacatcacc acaaaagaaa ttgcggcaat gctcaatgga tacatagacg agggcaaggc 2401 ggcgtcagcc aagttaatca gatcaacact gagcgatgca ttccgagagg caatagctga 2461 aggccatata acaacaaacc atgtcgctgc cactcgcgca gcaaaatcag aggtaaggag 2521 atcaagactt acggctgacg aatacctgaa aatttatcaa gcagcagaat catcaccatg 2581 ttggctcaga cttgcaatgg aactggctgt tgttaccggg caacgagttg gtgatttatg 2641 cgaaatgaag tggtctgata tcgtagatgg atatctttat gtcgagcaaa gcaaaacagg 2701 cgtaaaaatt gccatcccaa cagcattgca tattgatgct ctcggaatat caatgaagga 2761 aacacttgat aaatgcaaag agattcttgg cggagaaacc ataattgcat ctactcgtcg 2821 cgaaccgctt tcatccggca cagtatcaag gtattttatg cgcgcacgaa aagcatcagg 2881 tctttccttc gaaggagatc cgcctacctt tcacgagttg cgcagtttgt ctgcaagact 2941 ctatgagaag cagataagcg ataagtttgc tcaacatctt ctcgggcata agtcggacac 3001 catggcatca cagtatcgtg atgacagagg cagggagtgg gacaaaattg aaatcaaata 3061 aggatcttca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg tcagaatatc 3121 tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa ctgtttttcg 3181 aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt tttggtaact 3241 tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc gaggatattc 3301 ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa agccgggtcg 3361 aaaacgcttc gcccaaagac gagtaaggat ctctttaagg aaccggagga atcatgacca 3421 agtcagaatt gatagaaaga cttgccaccc agcaatcgca cattcccgcc aagacggttg 3481 aagatgcagt aaaagagatg ctggagcata tggcctcgac tcttgcgcag ggcgagcgta 3541 ttgaaatccg cggtttcggc agtttctctt tgcactaccg cgcaccacgt accggacgta 3601 atccgaagac tggcgataaa gtagaactgg aaggaaaata cgttcctcac tttaaacctg 3661 gtaaagaact gcggctagtg tcgaccatag tgactggata tgttgtgttt tacagtatta 3721 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt 3781 ttctcgttca gctttcttgt acaaagtggt tgatccttac agatcccggt tatccacaga 3841 atcaggggag gcctctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt 3901 tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt 3961 tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag 4021 tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc 4081 tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga 4141 ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat 4201 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca 4261 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca 4321 cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 4381 actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg 4441 gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt ccaggaaatc 4501 tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc 4561 agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca ccggtgcagc 4621 cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc aacatagtaa 4681 gccagtatac actccgctag cgctgatgtc cggcggtgcg catgccgtta agggattttg 4741 gtcatgaact agcaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 4801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 4861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 4921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 4981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 5041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 5101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 5161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 5221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 5281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 5341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 5401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 5461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 5521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 5581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 5641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 5701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 5761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 5821 tgccacctga cgtcctctgc agataacttc gtatagcata cattatacga agttatctcg 5881 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc 5941 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc 6001 aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc 6061 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt 6121 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt 6181 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat 6241 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg 6301 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa 6361 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag 6421 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat 6481 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt 6541 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa 6601 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc 6661 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga 6721 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaa //