LOCUS       BBa_K1122674            3622 bp    DNA     linear       25-Nov-2024
DEFINITION  Plac+fused PDC-ADH
ACCESSION   BBa_K1122674
VERSION     BBa_K1122674.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     CDS             609..3622
                     /label=BBa_K1122673
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagat taaagaggag aaatactaga tgtcttatac cgtggggacc tatctggcag
      661 aacgcctggt tcaaattggg ctgaaacacc acttcgctgt tgccggtgat tataacctgg
      721 tgctgctgga caatctgctg ctgaataaaa acatggagca agtctattgc tgtaacgaac
      781 tgaactgtgg ctttagcgct gaaggctatg cccgtgccaa aggtgccgcc gctgctgttg
      841 taacctatag tgtgggtgcc ctgagtgctt tcgatgctat tggaggggct tatgctgaaa
      901 acctgccggt aatcctgatt tctggtgccc cgaacaataa cgatcatgcc gccggtcatg
      961 tgctgcatca tgctctgggt aaaaccgact atcattatca gctggaaatg gccaaaaaca
     1021 ttactgccgc tgccgaggcg atttatactc cggaagaggc tccggccaaa attgatcatg
     1081 tcatcaaaac cgcactgcgt gagaaaaaac cggtctatct ggaaattgct tgtaacattg
     1141 cctcaatgcc gtgtgccgct cctggtccgg ctagtgccct gtttaacgat gaagcttcgg
     1201 atgaagcaag cctgaatgct gccgttgaag aaacgctgaa attcattgcc aaccgtgaca
     1261 aagtcgctgt actggtgggt tctaaactgc gcgctgctgg tgctgaggaa gccgctgtaa
     1321 aattcgctga cgccctgggt ggagctgtag caaccatggc tgccgccaaa tccttctttc
     1381 ctgaggagaa tcctcattat atcggcacga gctggggcga ggtttcatat ccaggggtgg
     1441 agaaaaccat gaaagaggca gacgccgtta tcgctctggc tcctgtgttc aatgactatt
     1501 ccaccaccgg gtggactgat attcctgacc cgaaaaaact ggtgctggct gaacctcgtt
     1561 ctgttgtggt gaatggcatc cgttttccga gcgttcacct gaaagactat ctgactcgtc
     1621 tggcccaaaa agtgtccaaa aaaactggcg ctctggactt ctttaaaagt ctgaatgccg
     1681 gggaactgaa aaaagctgct ccggccgatc cttctgctcc tctggttaat gccgagattg
     1741 ctcgtcaagt tgaagctctg ctgactccga ataccaccgt tatcgccgaa actggtgatt
     1801 cctggttcaa tgcccagcgt atgaaactgc cgaatggtgc tcgtgttgag tatgagatgc
     1861 aatggggcca tattggttgg tctgtgcctg ctgcttttgg ttatgccgtt ggtgctcctg
     1921 aacgtcgtaa catcctgatg gtaggggatg gatcatttca actgaccgcc caggaagttg
     1981 ctcaaatggt tcgtctgaaa ctgcctgtga tcatctttct gattaacaac tatggctata
     2041 cgatcgaggt catgattcac gatggtccgt ataacaacat taaaaactgg gactatgctg
     2101 gtctgatgga ggtgttcaat ggtaatggcg ggtatgattc tggagccggg aaaggcctga
     2161 aagcgaaaac cgggggtgag ctggctgagg ctattaaagt tgccctggcc aatacagatg
     2221 ggcctaccct gatcgaatgc tttattgggc gtgaggactg tacagaagaa ctggtaaaat
     2281 gggggaaacg tgttgccgct gctaacagcc gtaaaccggt gaataaactg ctggcatcaa
     2341 gcacctttta tatcccgttt gttaatgaaa tgggcgaggg ttctctggaa aaagcgatca
     2401 aagacctgaa tggtagtggg ttcaaaaatg ccctgattgt gtcagacgcc ttcatgaata
     2461 aaagtggcgt ggtgaaacag gttgctgatc tgctgaaagc acagggtatc aatagcgccg
     2521 tgtatgacgg tgttatgccg aatccgaccg ttacagccgt actggaggga ctgaaaatcc
     2581 tgaaagacaa caacagcgac ttcgtgattt ctctgggtgg tggttctcca catgattgtg
     2641 ccaaagccat tgccctggtg gctacaaatg gaggtgaagt gaaagattat gaggggatcg
     2701 acaaaagcaa aaaaccggca ctgcctctga tgagcatcaa taccacagcg ggtacagcat
     2761 ctgagatgac tcgtttctgt attatcaccg acgaggttcg ccatgtgaaa atggccatcg
     2821 tggatcgtca tgttacaccg atggttagcg tgaatgatcc tctgctgatg gtgggtatgc
     2881 ctaaagggct gactgccgct acaggaatgg acgccctgac tcatgccttc gaagcctatt
     2941 ctagcaccgc cgctactcct attaccgacg cttgtgccct gaaagctgcc tctatgattg
     3001 ccaaaaatct gaaaaccgct tgtgacaacg gcaaagacat gcctgctcgt gaggccatgg
     3061 cctatgccca atttctggcc ggtatggcgt ttaataacgc ctctctgggc tatgttcatg
     3121 ctatggctca ccaactggga ggctattata acctgccaca cggcgtctgt aatgctgtgc
     3181 tgctgcctca tgttctggcc tataatgcca gcgtggttgc tggtcgcctg aaagatgttg
     3241 gagtggctat gggtctggat attgccaatc tgggggacaa agagggtgct gaagcgacaa
     3301 ttcaagccgt ccgtgacctg gctgcttcta tcggaatccc ggcaaacctg acagaactgg
     3361 gcgccaaaaa agaagatgtg cctctgctgg ctgatcatgc tctgaaagac gcttgtgctc
     3421 tgactaatcc tcgtcaggga gatcagaaag aagtcgagga gctgtttctg agtgcctttt
     3481 gataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3541 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     3601 ggtgggcctt tctgcgttta ta
//