LOCUS BBa_K1122676 3660 bp DNA linear 01-Jun-2024 DEFINITION IPTG inducible pdc and adhB ACCESSION BBa_K1122676 VERSION BBa_K1122676.1 FEATURES Location/Qualifiers misc_feature 1..3 /label=SacI misc_feature 248..285 /label=CAP binding site misc_feature 332..352 /label=LacI binding site RBS 359..362 /label=rbs CDS 370..600 /label=lacZ' promoter 320..325 /label=-10 promoter 297..302 /label=-35 misc_feature 1..600 /label=BBa_J33207 RBS 609..623 /label=RBS-1\Strong RBS 616..620 /label=RBS misc_feature 609..623 /label=BBa_B0030 RBS 609..623 /label=BBa_B0030 CDS 630..2339 /label=BBa_K173016 RBS 2348..2362 /label=RBS-1\Strong RBS 2355..2359 /label=RBS misc_feature 2348..2362 /label=BBa_B0030 RBS 2348..2362 /label=BBa_B0030 CDS 2369..3523 /label=BBa_K173017 stem_loop 3543..3586 /label=stem_loop misc_feature 3532..3611 /label=BBa_B0010 terminator 3532..3611 /label=BBa_B0010 stem_loop 3627..3646 /label=T7 TE misc_feature 3653..3653 /label=stop polyA_site 3647..3660 /label=polya misc_feature 3620..3660 /label=BBa_B0012 terminator 3620..3660 /label=BBa_B0012 misc_feature 609..3660 /label=BBa_K173003 ORIGIN 1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag 361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg 421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga 601 tactagagat taaagaggag aaatactaga tgtcttatac cgtggggacc tatctggcag 661 aacgcctggt tcaaattggg ctgaaacacc acttcgctgt tgccggtgat tataacctgg 721 tgctgctgga caatctgctg ctgaataaaa acatggagca agtctattgc tgtaacgaac 781 tgaactgtgg ctttagcgct gaaggctatg cccgtgccaa aggtgccgcc gctgctgttg 841 taacctatag tgtgggtgcc ctgagtgctt tcgatgctat tggaggggct tatgctgaaa 901 acctgccggt aatcctgatt tctggtgccc cgaacaataa cgatcatgcc gccggtcatg 961 tgctgcatca tgctctgggt aaaaccgact atcattatca gctggaaatg gccaaaaaca 1021 ttactgccgc tgccgaggcg atttatactc cggaagaggc tccggccaaa attgatcatg 1081 tcatcaaaac cgcactgcgt gagaaaaaac cggtctatct ggaaattgct tgtaacattg 1141 cctcaatgcc gtgtgccgct cctggtccgg ctagtgccct gtttaacgat gaagcttcgg 1201 atgaagcaag cctgaatgct gccgttgaag aaacgctgaa attcattgcc aaccgtgaca 1261 aagtcgctgt actggtgggt tctaaactgc gcgctgctgg tgctgaggaa gccgctgtaa 1321 aattcgctga cgccctgggt ggagctgtag caaccatggc tgccgccaaa tccttctttc 1381 ctgaggagaa tcctcattat atcggcacga gctggggcga ggtttcatat ccaggggtgg 1441 agaaaaccat gaaagaggca gacgccgtta tcgctctggc tcctgtgttc aatgactatt 1501 ccaccaccgg gtggactgat attcctgacc cgaaaaaact ggtgctggct gaacctcgtt 1561 ctgttgtggt gaatggcatc cgttttccga gcgttcacct gaaagactat ctgactcgtc 1621 tggcccaaaa agtgtccaaa aaaactggcg ctctggactt ctttaaaagt ctgaatgccg 1681 gggaactgaa aaaagctgct ccggccgatc cttctgctcc tctggttaat gccgagattg 1741 ctcgtcaagt tgaagctctg ctgactccga ataccaccgt tatcgccgaa actggtgatt 1801 cctggttcaa tgcccagcgt atgaaactgc cgaatggtgc tcgtgttgag tatgagatgc 1861 aatggggcca tattggttgg tctgtgcctg ctgcttttgg ttatgccgtt ggtgctcctg 1921 aacgtcgtaa catcctgatg gtaggggatg gatcatttca actgaccgcc caggaagttg 1981 ctcaaatggt tcgtctgaaa ctgcctgtga tcatctttct gattaacaac tatggctata 2041 cgatcgaggt catgattcac gatggtccgt ataacaacat taaaaactgg gactatgctg 2101 gtctgatgga ggtgttcaat ggtaatggcg ggtatgattc tggagccggg aaaggcctga 2161 aagcgaaaac cgggggtgag ctggctgagg ctattaaagt tgccctggcc aatacagatg 2221 ggcctaccct gatcgaatgc tttattgggc gtgaggactg tacagaagaa ctggtaaaat 2281 gggggaaacg tgttgccgct gctaacagcc gtaaaccggt gaataaactg ctgtaataat 2341 actagagatt aaagaggaga aatactagat ggcatcaagc accttttata tcccgtttgt 2401 taatgaaatg ggcgagggtt ctctggaaaa agcgatcaaa gacctgaatg gtagtgggtt 2461 caaaaatgcc ctgattgtgt cagacgcctt catgaataaa agtggcgtgg tgaaacaggt 2521 tgctgatctg ctgaaagcac agggtatcaa tagcgccgtg tatgacggtg ttatgccgaa 2581 tccgaccgtt acagccgtac tggagggact gaaaatcctg aaagacaaca acagcgactt 2641 cgtgatttct ctgggtggtg gttctccaca tgattgtgcc aaagccattg ccctggtggc 2701 tacaaatgga ggtgaagtga aagattatga ggggatcgac aaaagcaaaa aaccggcact 2761 gcctctgatg agcatcaata ccacagcggg tacagcatct gagatgactc gtttctgtat 2821 tatcaccgac gaggttcgcc atgtgaaaat ggccatcgtg gatcgtcatg ttacaccgat 2881 ggttagcgtg aatgatcctc tgctgatggt gggtatgcct aaagggctga ctgccgctac 2941 aggaatggac gccctgactc atgccttcga agcctattct agcaccgccg ctactcctat 3001 taccgacgct tgtgccctga aagctgcctc tatgattgcc aaaaatctga aaaccgcttg 3061 tgacaacggc aaagacatgc ctgctcgtga ggccatggcc tatgcccaat ttctggccgg 3121 tatggcgttt aataacgcct ctctgggcta tgttcatgct atggctcacc aactgggagg 3181 ctattataac ctgccacacg gcgtctgtaa tgctgtgctg ctgcctcatg ttctggccta 3241 taatgccagc gtggttgctg gtcgcctgaa agatgttgga gtggctatgg gtctggatat 3301 tgccaatctg ggggacaaag agggtgctga agcgacaatt caagccgtcc gtgacctggc 3361 tgcttctatc ggaatcccgg caaacctgac agaactgggc gccaaaaaag aagatgtgcc 3421 tctgctggct gatcatgctc tgaaagacgc ttgtgctctg actaatcctc gtcagggaga 3481 tcagaaagaa gtcgaggagc tgtttctgag tgccttttga taatactaga gccaggcatc 3541 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 3601 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //