LOCUS BBa_K1122678 3450 bp DNA linear 31-Oct-2024 DEFINITION pSpac - LacZ - pdc- adhB ACCESSION BBa_K1122678 VERSION BBa_K1122678.1 FEATURES Location/Qualifiers misc_feature 1..390 /label=BBa_K1122677 RBS 399..413 /label=RBS-1\Strong RBS 406..410 /label=RBS misc_feature 399..413 /label=BBa_B0030 RBS 399..413 /label=BBa_B0030 CDS 420..2129 /label=BBa_K173016 RBS 2138..2152 /label=RBS-1\Strong RBS 2145..2149 /label=RBS misc_feature 2138..2152 /label=BBa_B0030 RBS 2138..2152 /label=BBa_B0030 CDS 2159..3313 /label=BBa_K173017 stem_loop 3333..3376 /label=stem_loop misc_feature 3322..3401 /label=BBa_B0010 terminator 3322..3401 /label=BBa_B0010 stem_loop 3417..3436 /label=T7 TE misc_feature 3443..3443 /label=stop polyA_site 3437..3450 /label=polya misc_feature 3410..3450 /label=BBa_B0012 terminator 3410..3450 /label=BBa_B0012 misc_feature 399..3450 /label=BBa_K173003 ORIGIN 1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 61 cagcgtggac cgcttgcaaa aagttgttga ctttatctac aaggtgtggc ataatgtgtg 121 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacggattca 181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 301 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca 361 gaagcggtgc cggaaagctg gctggagtga tactagagat taaagaggag aaatactaga 421 tgtcttatac cgtggggacc tatctggcag aacgcctggt tcaaattggg ctgaaacacc 481 acttcgctgt tgccggtgat tataacctgg tgctgctgga caatctgctg ctgaataaaa 541 acatggagca agtctattgc tgtaacgaac tgaactgtgg ctttagcgct gaaggctatg 601 cccgtgccaa aggtgccgcc gctgctgttg taacctatag tgtgggtgcc ctgagtgctt 661 tcgatgctat tggaggggct tatgctgaaa acctgccggt aatcctgatt tctggtgccc 721 cgaacaataa cgatcatgcc gccggtcatg tgctgcatca tgctctgggt aaaaccgact 781 atcattatca gctggaaatg gccaaaaaca ttactgccgc tgccgaggcg atttatactc 841 cggaagaggc tccggccaaa attgatcatg tcatcaaaac cgcactgcgt gagaaaaaac 901 cggtctatct ggaaattgct tgtaacattg cctcaatgcc gtgtgccgct cctggtccgg 961 ctagtgccct gtttaacgat gaagcttcgg atgaagcaag cctgaatgct gccgttgaag 1021 aaacgctgaa attcattgcc aaccgtgaca aagtcgctgt actggtgggt tctaaactgc 1081 gcgctgctgg tgctgaggaa gccgctgtaa aattcgctga cgccctgggt ggagctgtag 1141 caaccatggc tgccgccaaa tccttctttc ctgaggagaa tcctcattat atcggcacga 1201 gctggggcga ggtttcatat ccaggggtgg agaaaaccat gaaagaggca gacgccgtta 1261 tcgctctggc tcctgtgttc aatgactatt ccaccaccgg gtggactgat attcctgacc 1321 cgaaaaaact ggtgctggct gaacctcgtt ctgttgtggt gaatggcatc cgttttccga 1381 gcgttcacct gaaagactat ctgactcgtc tggcccaaaa agtgtccaaa aaaactggcg 1441 ctctggactt ctttaaaagt ctgaatgccg gggaactgaa aaaagctgct ccggccgatc 1501 cttctgctcc tctggttaat gccgagattg ctcgtcaagt tgaagctctg ctgactccga 1561 ataccaccgt tatcgccgaa actggtgatt cctggttcaa tgcccagcgt atgaaactgc 1621 cgaatggtgc tcgtgttgag tatgagatgc aatggggcca tattggttgg tctgtgcctg 1681 ctgcttttgg ttatgccgtt ggtgctcctg aacgtcgtaa catcctgatg gtaggggatg 1741 gatcatttca actgaccgcc caggaagttg ctcaaatggt tcgtctgaaa ctgcctgtga 1801 tcatctttct gattaacaac tatggctata cgatcgaggt catgattcac gatggtccgt 1861 ataacaacat taaaaactgg gactatgctg gtctgatgga ggtgttcaat ggtaatggcg 1921 ggtatgattc tggagccggg aaaggcctga aagcgaaaac cgggggtgag ctggctgagg 1981 ctattaaagt tgccctggcc aatacagatg ggcctaccct gatcgaatgc tttattgggc 2041 gtgaggactg tacagaagaa ctggtaaaat gggggaaacg tgttgccgct gctaacagcc 2101 gtaaaccggt gaataaactg ctgtaataat actagagatt aaagaggaga aatactagat 2161 ggcatcaagc accttttata tcccgtttgt taatgaaatg ggcgagggtt ctctggaaaa 2221 agcgatcaaa gacctgaatg gtagtgggtt caaaaatgcc ctgattgtgt cagacgcctt 2281 catgaataaa agtggcgtgg tgaaacaggt tgctgatctg ctgaaagcac agggtatcaa 2341 tagcgccgtg tatgacggtg ttatgccgaa tccgaccgtt acagccgtac tggagggact 2401 gaaaatcctg aaagacaaca acagcgactt cgtgatttct ctgggtggtg gttctccaca 2461 tgattgtgcc aaagccattg ccctggtggc tacaaatgga ggtgaagtga aagattatga 2521 ggggatcgac aaaagcaaaa aaccggcact gcctctgatg agcatcaata ccacagcggg 2581 tacagcatct gagatgactc gtttctgtat tatcaccgac gaggttcgcc atgtgaaaat 2641 ggccatcgtg gatcgtcatg ttacaccgat ggttagcgtg aatgatcctc tgctgatggt 2701 gggtatgcct aaagggctga ctgccgctac aggaatggac gccctgactc atgccttcga 2761 agcctattct agcaccgccg ctactcctat taccgacgct tgtgccctga aagctgcctc 2821 tatgattgcc aaaaatctga aaaccgcttg tgacaacggc aaagacatgc ctgctcgtga 2881 ggccatggcc tatgcccaat ttctggccgg tatggcgttt aataacgcct ctctgggcta 2941 tgttcatgct atggctcacc aactgggagg ctattataac ctgccacacg gcgtctgtaa 3001 tgctgtgctg ctgcctcatg ttctggccta taatgccagc gtggttgctg gtcgcctgaa 3061 agatgttgga gtggctatgg gtctggatat tgccaatctg ggggacaaag agggtgctga 3121 agcgacaatt caagccgtcc gtgacctggc tgcttctatc ggaatcccgg caaacctgac 3181 agaactgggc gccaaaaaag aagatgtgcc tctgctggct gatcatgctc tgaaagacgc 3241 ttgtgctctg actaatcctc gtcagggaga tcagaaagaa gtcgaggagc tgtttctgag 3301 tgccttttga taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3361 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3421 caccttcggg tgggcctttc tgcgtttata //