LOCUS       BBa_K1122678            3450 bp    DNA     linear       01-Jun-2024
DEFINITION  pSpac - LacZ - pdc- adhB
ACCESSION   BBa_K1122678
VERSION     BBa_K1122678.1  
FEATURES             Location/Qualifiers
     misc_feature    1..390
                     /label=BBa_K1122677
     RBS             399..413
                     /label=RBS-1\Strong
     RBS             406..410
                     /label=RBS
     misc_feature    399..413
                     /label=BBa_B0030
     RBS             399..413
                     /label=BBa_B0030
     CDS             420..2129
                     /label=BBa_K173016
     RBS             2138..2152
                     /label=RBS-1\Strong
     RBS             2145..2149
                     /label=RBS
     misc_feature    2138..2152
                     /label=BBa_B0030
     RBS             2138..2152
                     /label=BBa_B0030
     CDS             2159..3313
                     /label=BBa_K173017
     stem_loop       3333..3376
                     /label=stem_loop
     misc_feature    3322..3401
                     /label=BBa_B0010
     terminator      3322..3401
                     /label=BBa_B0010
     stem_loop       3417..3436
                     /label=T7 TE
     misc_feature    3443..3443
                     /label=stop
     polyA_site      3437..3450
                     /label=polya
     misc_feature    3410..3450
                     /label=BBa_B0012
     terminator      3410..3450
                     /label=BBa_B0012
     misc_feature    399..3450
                     /label=BBa_K173003
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgcaaa aagttgttga ctttatctac aaggtgtggc ataatgtgtg
      121 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacggattca
      181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
      241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
      301 ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca
      361 gaagcggtgc cggaaagctg gctggagtga tactagagat taaagaggag aaatactaga
      421 tgtcttatac cgtggggacc tatctggcag aacgcctggt tcaaattggg ctgaaacacc
      481 acttcgctgt tgccggtgat tataacctgg tgctgctgga caatctgctg ctgaataaaa
      541 acatggagca agtctattgc tgtaacgaac tgaactgtgg ctttagcgct gaaggctatg
      601 cccgtgccaa aggtgccgcc gctgctgttg taacctatag tgtgggtgcc ctgagtgctt
      661 tcgatgctat tggaggggct tatgctgaaa acctgccggt aatcctgatt tctggtgccc
      721 cgaacaataa cgatcatgcc gccggtcatg tgctgcatca tgctctgggt aaaaccgact
      781 atcattatca gctggaaatg gccaaaaaca ttactgccgc tgccgaggcg atttatactc
      841 cggaagaggc tccggccaaa attgatcatg tcatcaaaac cgcactgcgt gagaaaaaac
      901 cggtctatct ggaaattgct tgtaacattg cctcaatgcc gtgtgccgct cctggtccgg
      961 ctagtgccct gtttaacgat gaagcttcgg atgaagcaag cctgaatgct gccgttgaag
     1021 aaacgctgaa attcattgcc aaccgtgaca aagtcgctgt actggtgggt tctaaactgc
     1081 gcgctgctgg tgctgaggaa gccgctgtaa aattcgctga cgccctgggt ggagctgtag
     1141 caaccatggc tgccgccaaa tccttctttc ctgaggagaa tcctcattat atcggcacga
     1201 gctggggcga ggtttcatat ccaggggtgg agaaaaccat gaaagaggca gacgccgtta
     1261 tcgctctggc tcctgtgttc aatgactatt ccaccaccgg gtggactgat attcctgacc
     1321 cgaaaaaact ggtgctggct gaacctcgtt ctgttgtggt gaatggcatc cgttttccga
     1381 gcgttcacct gaaagactat ctgactcgtc tggcccaaaa agtgtccaaa aaaactggcg
     1441 ctctggactt ctttaaaagt ctgaatgccg gggaactgaa aaaagctgct ccggccgatc
     1501 cttctgctcc tctggttaat gccgagattg ctcgtcaagt tgaagctctg ctgactccga
     1561 ataccaccgt tatcgccgaa actggtgatt cctggttcaa tgcccagcgt atgaaactgc
     1621 cgaatggtgc tcgtgttgag tatgagatgc aatggggcca tattggttgg tctgtgcctg
     1681 ctgcttttgg ttatgccgtt ggtgctcctg aacgtcgtaa catcctgatg gtaggggatg
     1741 gatcatttca actgaccgcc caggaagttg ctcaaatggt tcgtctgaaa ctgcctgtga
     1801 tcatctttct gattaacaac tatggctata cgatcgaggt catgattcac gatggtccgt
     1861 ataacaacat taaaaactgg gactatgctg gtctgatgga ggtgttcaat ggtaatggcg
     1921 ggtatgattc tggagccggg aaaggcctga aagcgaaaac cgggggtgag ctggctgagg
     1981 ctattaaagt tgccctggcc aatacagatg ggcctaccct gatcgaatgc tttattgggc
     2041 gtgaggactg tacagaagaa ctggtaaaat gggggaaacg tgttgccgct gctaacagcc
     2101 gtaaaccggt gaataaactg ctgtaataat actagagatt aaagaggaga aatactagat
     2161 ggcatcaagc accttttata tcccgtttgt taatgaaatg ggcgagggtt ctctggaaaa
     2221 agcgatcaaa gacctgaatg gtagtgggtt caaaaatgcc ctgattgtgt cagacgcctt
     2281 catgaataaa agtggcgtgg tgaaacaggt tgctgatctg ctgaaagcac agggtatcaa
     2341 tagcgccgtg tatgacggtg ttatgccgaa tccgaccgtt acagccgtac tggagggact
     2401 gaaaatcctg aaagacaaca acagcgactt cgtgatttct ctgggtggtg gttctccaca
     2461 tgattgtgcc aaagccattg ccctggtggc tacaaatgga ggtgaagtga aagattatga
     2521 ggggatcgac aaaagcaaaa aaccggcact gcctctgatg agcatcaata ccacagcggg
     2581 tacagcatct gagatgactc gtttctgtat tatcaccgac gaggttcgcc atgtgaaaat
     2641 ggccatcgtg gatcgtcatg ttacaccgat ggttagcgtg aatgatcctc tgctgatggt
     2701 gggtatgcct aaagggctga ctgccgctac aggaatggac gccctgactc atgccttcga
     2761 agcctattct agcaccgccg ctactcctat taccgacgct tgtgccctga aagctgcctc
     2821 tatgattgcc aaaaatctga aaaccgcttg tgacaacggc aaagacatgc ctgctcgtga
     2881 ggccatggcc tatgcccaat ttctggccgg tatggcgttt aataacgcct ctctgggcta
     2941 tgttcatgct atggctcacc aactgggagg ctattataac ctgccacacg gcgtctgtaa
     3001 tgctgtgctg ctgcctcatg ttctggccta taatgccagc gtggttgctg gtcgcctgaa
     3061 agatgttgga gtggctatgg gtctggatat tgccaatctg ggggacaaag agggtgctga
     3121 agcgacaatt caagccgtcc gtgacctggc tgcttctatc ggaatcccgg caaacctgac
     3181 agaactgggc gccaaaaaag aagatgtgcc tctgctggct gatcatgctc tgaaagacgc
     3241 ttgtgctctg actaatcctc gtcagggaga tcagaaagaa gtcgaggagc tgtttctgag
     3301 tgccttttga taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     3361 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     3421 caccttcggg tgggcctttc tgcgtttata
//