BBa_K173017
1
adhB
alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)
2009-10-14T11:00:00Z
2015-05-08T01:10:57Z
a
a
false
false
_281_
0
2583
9
Not in stock
true
a
false
Lorenzo Pasotti, Paolo Magni
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1122678
1
BBa_K1122678
pSpac - LacZ - pdc- adhB
2013-08-29T11:00:00Z
2015-05-08T01:09:15Z
synthetic
This translational unit enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) in ''Bacillus subtilis'' using xylose induction. Production of pdc and adhB enzymes allows for conversion of pyruvate to ethanol via an acetaldehyde intermediate.
false
false
_1433_
0
18014
9
Not in stock
false
Part was made by conversion of EdiBrick - pdc - adhB translational unit by MABEL using standardised primers.
false
Aleksandra Lewicka, Jan Lyczakowski
component2333655
1
BBa_K173003
component2333640
1
BBa_K1122677
annotation2333640
1
BBa_K1122677
range2333640
1
1
390
annotation2333655
1
BBa_K173003
range2333655
1
399
3450
BBa_K173016
1
pdc
pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)
2009-10-14T11:00:00Z
2015-05-08T01:10:57Z
a
a
false
false
_281_
0
2583
9
Not in stock
true
a
false
Lorenzo Pasotti, Paolo Magni
BBa_K173003
1
BBa_K173003
pdc and adhB protein generator
2009-10-15T11:00:00Z
2015-05-08T01:10:57Z
a
a
false
false
_281_
0
2583
9
It's complicated
true
a
false
Lorenzo Pasotti, Giulio Chiesa, Manuel Lupotto, Paolo Magni
component2044293
1
BBa_K173017
component2044289
1
BBa_K173016
component2044291
1
BBa_B0030
component2044294
1
BBa_B0010
component2044287
1
BBa_B0030
component2044296
1
BBa_B0012
annotation2044291
1
BBa_B0030
range2044291
1
1740
1754
annotation2044287
1
BBa_B0030
range2044287
1
1
15
annotation2044296
1
BBa_B0012
range2044296
1
3012
3052
annotation2044293
1
BBa_K173017
range2044293
1
1761
2915
annotation2044289
1
BBa_K173016
range2044289
1
22
1731
annotation2044294
1
BBa_B0010
range2044294
1
2924
3003
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_K1122677
1
BBa_K1122677
pSpac - LacZ
2013-08-29T11:00:00Z
2015-05-08T01:09:15Z
synthetic
This part encodes a pSpac - xylose inducible promoter for ''B. subtilis'' followed by LacZ alpha peptide.
false
false
_1433_
0
18014
9
Not in stock
false
The part can be generated from any EdiBrick (J33207) controlled part with a single MABEL process using standardised primers
false
Aleksandra Lewicka
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1122678_sequence
1
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagattaaagaggagaaatactagatgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggcctaccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaacagccgtaaaccggtgaataaactgctgtaataatactagagattaaagaggagaaatactagatggcatcaagcaccttttatatcccgtttgttaatgaaatgggcgagggttctctggaaaaagcgatcaaagacctgaatggtagtgggttcaaaaatgccctgattgtgtcagacgccttcatgaataaaagtggcgtggtgaaacaggttgctgatctgctgaaagcacagggtatcaatagcgccgtgtatgacggtgttatgccgaatccgaccgttacagccgtactggagggactgaaaatcctgaaagacaacaacagcgacttcgtgatttctctgggtggtggttctccacatgattgtgccaaagccattgccctggtggctacaaatggaggtgaagtgaaagattatgaggggatcgacaaaagcaaaaaaccggcactgcctctgatgagcatcaataccacagcgggtacagcatctgagatgactcgtttctgtattatcaccgacgaggttcgccatgtgaaaatggccatcgtggatcgtcatgttacaccgatggttagcgtgaatgatcctctgctgatggtgggtatgcctaaagggctgactgccgctacaggaatggacgccctgactcatgccttcgaagcctattctagcaccgccgctactcctattaccgacgcttgtgccctgaaagctgcctctatgattgccaaaaatctgaaaaccgcttgtgacaacggcaaagacatgcctgctcgtgaggccatggcctatgcccaatttctggccggtatggcgtttaataacgcctctctgggctatgttcatgctatggctcaccaactgggaggctattataacctgccacacggcgtctgtaatgctgtgctgctgcctcatgttctggcctataatgccagcgtggttgctggtcgcctgaaagatgttggagtggctatgggtctggatattgccaatctgggggacaaagagggtgctgaagcgacaattcaagccgtccgtgacctggctgcttctatcggaatcccggcaaacctgacagaactgggcgccaaaaaagaagatgtgcctctgctggctgatcatgctctgaaagacgcttgtgctctgactaatcctcgtcagggagatcagaaagaagtcgaggagctgtttctgagtgccttttgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K173016_sequence
1
atgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggcctaccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaacagccgtaaaccggtgaataaactgctgtaataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K173003_sequence
1
attaaagaggagaaatactagatgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggcctaccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaacagccgtaaaccggtgaataaactgctgtaataatactagagattaaagaggagaaatactagatggcatcaagcaccttttatatcccgtttgttaatgaaatgggcgagggttctctggaaaaagcgatcaaagacctgaatggtagtgggttcaaaaatgccctgattgtgtcagacgccttcatgaataaaagtggcgtggtgaaacaggttgctgatctgctgaaagcacagggtatcaatagcgccgtgtatgacggtgttatgccgaatccgaccgttacagccgtactggagggactgaaaatcctgaaagacaacaacagcgacttcgtgatttctctgggtggtggttctccacatgattgtgccaaagccattgccctggtggctacaaatggaggtgaagtgaaagattatgaggggatcgacaaaagcaaaaaaccggcactgcctctgatgagcatcaataccacagcgggtacagcatctgagatgactcgtttctgtattatcaccgacgaggttcgccatgtgaaaatggccatcgtggatcgtcatgttacaccgatggttagcgtgaatgatcctctgctgatggtgggtatgcctaaagggctgactgccgctacaggaatggacgccctgactcatgccttcgaagcctattctagcaccgccgctactcctattaccgacgcttgtgccctgaaagctgcctctatgattgccaaaaatctgaaaaccgcttgtgacaacggcaaagacatgcctgctcgtgaggccatggcctatgcccaatttctggccggtatggcgtttaataacgcctctctgggctatgttcatgctatggctcaccaactgggaggctattataacctgccacacggcgtctgtaatgctgtgctgctgcctcatgttctggcctataatgccagcgtggttgctggtcgcctgaaagatgttggagtggctatgggtctggatattgccaatctgggggacaaagagggtgctgaagcgacaattcaagccgtccgtgacctggctgcttctatcggaatcccggcaaacctgacagaactgggcgccaaaaaagaagatgtgcctctgctggctgatcatgctctgaaagacgcttgtgctctgactaatcctcgtcagggagatcagaaagaagtcgaggagctgtttctgagtgccttttgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1122677_sequence
1
ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K173017_sequence
1
atggcatcaagcaccttttatatcccgtttgttaatgaaatgggcgagggttctctggaaaaagcgatcaaagacctgaatggtagtgggttcaaaaatgccctgattgtgtcagacgccttcatgaataaaagtggcgtggtgaaacaggttgctgatctgctgaaagcacagggtatcaatagcgccgtgtatgacggtgttatgccgaatccgaccgttacagccgtactggagggactgaaaatcctgaaagacaacaacagcgacttcgtgatttctctgggtggtggttctccacatgattgtgccaaagccattgccctggtggctacaaatggaggtgaagtgaaagattatgaggggatcgacaaaagcaaaaaaccggcactgcctctgatgagcatcaataccacagcgggtacagcatctgagatgactcgtttctgtattatcaccgacgaggttcgccatgtgaaaatggccatcgtggatcgtcatgttacaccgatggttagcgtgaatgatcctctgctgatggtgggtatgcctaaagggctgactgccgctacaggaatggacgccctgactcatgccttcgaagcctattctagcaccgccgctactcctattaccgacgcttgtgccctgaaagctgcctctatgattgccaaaaatctgaaaaccgcttgtgacaacggcaaagacatgcctgctcgtgaggccatggcctatgcccaatttctggccggtatggcgtttaataacgcctctctgggctatgttcatgctatggctcaccaactgggaggctattataacctgccacacggcgtctgtaatgctgtgctgctgcctcatgttctggcctataatgccagcgtggttgctggtcgcctgaaagatgttggagtggctatgggtctggatattgccaatctgggggacaaagagggtgctgaagcgacaattcaagccgtccgtgacctggctgcttctatcggaatcccggcaaacctgacagaactgggcgccaaaaaagaagatgtgcctctgctggctgatcatgctctgaaagacgcttgtgctctgactaatcctcgtcagggagatcagaaagaagtcgaggagctgtttctgagtgccttttgataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z