BBa_K1122678
type: ComponentDefinition
persistent identity: BBa K1122678
display id: BBa_K1122678
version: 1
was generated by: iGEM to SBOL conversion
description: pSpac - LacZ - pdc- adhB
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:15Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This translational unit enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) in ''Bacillus subtilis'' using xylose induction. Production of pdc and adhB enzymes allows for conversion of pyruvate to ethanol via an acetaldehyde intermediate.
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 0
owner id: 18014
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Part was made by conversion of EdiBrick - pdc - adhB translational unit by MABEL using standardised primers.
star: false
creator: Aleksandra Lewicka, Jan Lyczakowski
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K173003
component: BBa_K1122677
sequence annotation: BBa_K1122677
sequence annotation: BBa_K173003
sequence: https://synbiohub.org/public/igem/BBa_K1122678_sequence/1

BBa_K173003
type: Component
persistent identity: component2333655
display id: component2333655
version: 1
top level: BBa_K1122678
definition: BBa_K173003
access: public

BBa_K1122677
type: Component
persistent identity: component2333640
display id: component2333640
version: 1
top level: BBa_K1122678
definition: BBa_K1122677
access: public

BBa_K1122677
type: SequenceAnnotation
persistent identity: annotation2333640
display id: annotation2333640
version: 1
top level: BBa_K1122678
location: https://synbiohub.org/public/igem/BBa_K1122678/annotation2333640/range2333640/1
component: BBa_K1122677

https://synbiohub.org/public/igem/BBa_K1122678/annotation2333640/range2333640/1
type: Range
persistent identity: range2333640
display id: range2333640
version: 1
top level: BBa_K1122678
direction: direction/reverse
start: 1
end: 390
orientation: inline

BBa_K173003
type: SequenceAnnotation
persistent identity: annotation2333655
display id: annotation2333655
version: 1
top level: BBa_K1122678
location: https://synbiohub.org/public/igem/BBa_K1122678/annotation2333655/range2333655/1
component: BBa_K173003

https://synbiohub.org/public/igem/BBa_K1122678/annotation2333655/range2333655/1
type: Range
persistent identity: range2333655
display id: range2333655
version: 1
top level: BBa_K1122678
direction: direction/reverse
start: 399
end: 3450
orientation: inline

BBa_K1122677
type: ComponentDefinition
persistent identity: BBa K1122677
display id: BBa_K1122677
version: 1
was generated by: iGEM to SBOL conversion
description: pSpac - LacZ
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:09:15Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This part encodes a pSpac - xylose inducible promoter for ''B. subtilis'' followed by LacZ alpha peptide.
discontinued: false
dominant: false
experience: experience/None
group u list: _1433_
m user id: 0
owner id: 18014
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part can be generated from any EdiBrick (J33207) controlled part with a single MABEL process using standardised primers
star: false
creator: Aleksandra Lewicka
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence: https://synbiohub.org/public/igem/BBa_K1122677_sequence/1

BBa_K173003
type: ComponentDefinition
persistent identity: BBa K173003
display id: BBa_K173003
version: 1
was generated by: iGEM to SBOL conversion
description: pdc and adhB protein generator
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:10:57Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/Works
group u list: _281_
m user id: 0
owner id: 2583
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: a
star: false
creator: Lorenzo Pasotti, Giulio Chiesa, Manuel Lupotto, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_B0010
component: BBa_K173016
component: BBa_B0030
component: BBa_K173017
sequence annotation: BBa_B0030
sequence annotation: BBa_K173016
sequence annotation: BBa_K173017
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K173003_sequence/1

BBa_B0012
type: Component
persistent identity: component2044296
display id: component2044296
version: 1
top level: BBa_K173003
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2044294
display id: component2044294
version: 1
top level: BBa_K173003
definition: BBa_B0010
access: public

BBa_K173016
type: Component
persistent identity: component2044289
display id: component2044289
version: 1
top level: BBa_K173003
definition: pdc
access: public

BBa_B0030
type: Component
persistent identity: component2044287
display id: component2044287
version: 1
top level: BBa_K173003
definition: BBa_B0030
access: public

BBa_K173017
type: Component
persistent identity: component2044293
display id: component2044293
version: 1
top level: BBa_K173003
definition: adhB
access: public

BBa_B0030
type: Component
persistent identity: component2044291
display id: component2044291
version: 1
top level: BBa_K173003
definition: BBa_B0030
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2044287
display id: annotation2044287
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044287/range2044287/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K173003/annotation2044287/range2044287/1
type: Range
persistent identity: range2044287
display id: range2044287
version: 1
top level: BBa_K173003
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_K173016
type: SequenceAnnotation
persistent identity: annotation2044289
display id: annotation2044289
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044289/range2044289/1
component: BBa_K173016

https://synbiohub.org/public/igem/BBa_K173003/annotation2044289/range2044289/1
type: Range
persistent identity: range2044289
display id: range2044289
version: 1
top level: BBa_K173003
direction: direction/reverse
start: 22
end: 1731
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2044291
display id: annotation2044291
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044291/range2044291/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K173003/annotation2044291/range2044291/1
type: Range
persistent identity: range2044291
display id: range2044291
version: 1
top level: BBa_K173003
direction: direction/forward
start: 1740
end: 1754
orientation: inline

BBa_K173017
type: SequenceAnnotation
persistent identity: annotation2044293
display id: annotation2044293
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044293/range2044293/1
component: BBa_K173017

https://synbiohub.org/public/igem/BBa_K173003/annotation2044293/range2044293/1
type: Range
persistent identity: range2044293
display id: range2044293
version: 1
top level: BBa_K173003
direction: direction/reverse
start: 1761
end: 2915
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2044294
display id: annotation2044294
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044294/range2044294/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K173003/annotation2044294/range2044294/1
type: Range
persistent identity: range2044294
display id: range2044294
version: 1
top level: BBa_K173003
direction: direction/forward
start: 2924
end: 3003
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2044296
display id: annotation2044296
version: 1
top level: BBa_K173003
location: https://synbiohub.org/public/igem/BBa_K173003/annotation2044296/range2044296/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K173003/annotation2044296/range2044296/1
type: Range
persistent identity: range2044296
display id: range2044296
version: 1
top level: BBa_K173003
direction: direction/forward
start: 3012
end: 3052
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

pdc
type: ComponentDefinition
persistent identity: BBa K173016
display id: BBa_K173016
version: 1
was generated by: iGEM to SBOL conversion
description: pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:10:57Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _281_
m user id: 0
owner id: 2583
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: a
star: false
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K173016_sequence/1

adhB
type: ComponentDefinition
persistent identity: BBa K173017
display id: BBa_K173017
version: 1
was generated by: iGEM to SBOL conversion
description: alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)
created: 2009-10-14T11:00:00Z
modified: 2015-05-08T01:10:57Z
mutable provenance: a
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _281_
m user id: 0
owner id: 2583
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: a
star: false
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K173017_sequence/1

https://synbiohub.org/public/igem/BBa_K1122678_sequence/1
type: Sequence
persistent identity: BBa K1122678 sequence
display id: BBa_K1122678_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgatactagagattaaagaggagaaatactagatgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggcc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1122677_sequence/1
type: Sequence
persistent identity: BBa K1122677 sequence
display id: BBa_K1122677_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K173003_sequence/1
type: Sequence
persistent identity: BBa K173003 sequence
display id: BBa_K173003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggcctaccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaacagccgtaaaccggtgaataaactgctgtaataatactagagattaaagaggagaaatactagatggcatcaagcaccttttatatcccgtttgttaatgaaatgggcgagggttctctggaaaaagcgatcaaagacctgaatggtagtgggttcaaaaatgccctgattgtgtcagacgccttcatgaataaaagtggcgtggtgaaacaggttgctgatctgctgaaagcacagggtatcaatagcgccgtgtatgacggtgttatgccgaatccgaccgttacagccgtactggaggga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K173016_sequence/1
type: Sequence
persistent identity: BBa K173016 sequence
display id: BBa_K173016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcttataccgtggggacctatctggcagaacgcctggttcaaattgggctgaaacaccacttcgctgttgccggtgattataacctggtgctgctggacaatctgctgctgaataaaaacatggagcaagtctattgctgtaacgaactgaactgtggctttagcgctgaaggctatgcccgtgccaaaggtgccgccgctgctgttgtaacctatagtgtgggtgccctgagtgctttcgatgctattggaggggcttatgctgaaaacctgccggtaatcctgatttctggtgccccgaacaataacgatcatgccgccggtcatgtgctgcatcatgctctgggtaaaaccgactatcattatcagctggaaatggccaaaaacattactgccgctgccgaggcgatttatactccggaagaggctccggccaaaattgatcatgtcatcaaaaccgcactgcgtgagaaaaaaccggtctatctggaaattgcttgtaacattgcctcaatgccgtgtgccgctcctggtccggctagtgccctgtttaacgatgaagcttcggatgaagcaagcctgaatgctgccgttgaagaaacgctgaaattcattgccaaccgtgacaaagtcgctgtactggtgggttctaaactgcgcgctgctggtgctgaggaagccgctgtaaaattcgctgacgccctgggtggagctgtagcaaccatggctgccgccaaatccttctttcctgaggagaatcctcattatatcggcacgagctggggcgaggtttcatatccaggggtggagaaaaccatgaaagaggcagacgccgttatcgctctggctcctgtgttcaatgactattccaccaccgggtggactgatattcctgacccgaaaaaactggtgctggctgaacctcgttctgttgtggtgaatggcatccgttttccgagcgttcacctgaaagactatctgactcgtctggcccaaaaagtgtccaaaaaaactggcgctctggacttctttaaaagtctgaatgccggggaactgaaaaaagctgctccggccgatccttctgctcctctggttaatgccgagattgctcgtcaagttgaagctctgctgactccgaataccaccgttatcgccgaaactggtgattcctggttcaatgcccagcgtatgaaactgccgaatggtgctcgtgttgagtatgagatgcaatggggccatattggttggtctgtgcctgctgcttttggttatgccgttggtgctcctgaacgtcgtaacatcctgatggtaggggatggatcatttcaactgaccgcccaggaagttgctcaaatggttcgtctgaaactgcctgtgatcatctttctgattaacaactatggctatacgatcgaggtcatgattcacgatggtccgtataacaacattaaaaactgggactatgctggtctgatggaggtgttcaatggtaatggcgggtatgattctggagccgggaaaggcctgaaagcgaaaaccgggggtgagctggctgaggctattaaagttgccctggccaatacagatgggcctaccctgatcgaatgctttattgggcgtgaggactgtacagaagaactggtaaaatgggggaaacgtgttgccgctgctaacagccgtaaaccggtgaataaactgctgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K173017_sequence/1
type: Sequence
persistent identity: BBa K173017 sequence
display id: BBa_K173017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcatcaagcaccttttatatcccgtttgttaatgaaatgggcgagggttctctggaaaaagcgatcaaagacctgaatggtagtgggttcaaaaatgccctgattgtgtcagacgccttcatgaataaaagtggcgtggtgaaacaggttgctgatctgctgaaagcacagggtatcaatagcgccgtgtatgacggtgttatgccgaatccgaccgttacagccgtactggagggactgaaaatcctgaaagacaacaacagcgacttcgtgatttctctgggtggtggttctccacatgattgtgccaaagccattgccctggtggctacaaatggaggtgaagtgaaagattatgaggggatcgacaaaagcaaaaaaccggcactgcctctgatgagcatcaataccacagcgggtacagcatctgagatgactcgtttctgtattatcaccgacgaggttcgccatgtgaaaatggccatcgtggatcgtcatgttacaccgatggttagcgtgaatgatcctctgctgatggtgggtatgcctaaagggctgactgccgctacaggaatggacgccctgactcatgccttcgaagcctattctagcaccgccgctactcctattaccgacgcttgtgccctgaaagctgcctctatgattgccaaaaatctgaaaaccgcttgtgacaacggcaaagacatgcctgctcgtgaggccatggcctatgcccaatttctggccggtatggcgtttaataacgcctctctgggctatgttcatgctatggctcaccaactgggaggctattataacctgccacacggcgtctgtaatgctgtgctgctgcctcatgttctggcctataatgccagcgtggttgctggtcgcctgaaagatgttggagtggctatgggtctggatattgccaatctgggggacaaagagggtgctgaagcgacaattcaagccgtccgtgacctggctgcttctatcggaatcccggcaaacctgacagaactgggcgccaaaaaagaagatgtgcctctgctggctgatcatgctctgaaagacgcttgtgctctgactaatcctcgtcagggagatcagaaagaagtcgaggagctgtttctgagtgccttttgataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z