This translational unit enables expression of pyruvate decarboxylase (pdc) and alcohol dehydrogenase B (adhB) in ''Bacillus subtilis'' using xylose induction. Production of pdc and adhB enzymes allows for conversion of pyruvate to ethanol via an acetaldehyde intermediate.
Part was made by conversion of EdiBrick - pdc - adhB translational unit by MABEL using standardised primers.
This part encodes a pSpac - xylose inducible promoter for ''B. subtilis'' followed by LacZ alpha peptide.
The part can be generated from any EdiBrick (J33207) controlled part with a single MABEL process using standardised primers
Lorenzo Pasotti, Giulio Chiesa, Manuel Lupotto, Paolo Magni
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
RBS.1 (strong) -- modified from R. Weiss
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
pyruvate decarboxylase from Z. mobilis (codon optimized for E. coli)
alcohol dehydrogenase II from Z. mobilis (codon optimized for E. coli)
Conversion of the iGEM parts registry to SBOL2.1