BBa_K1123018
1
BBa_K1123018
Poly(Arginine-Glycine) Protein
2013-08-30T11:00:00Z
2015-05-08T01:09:16Z
We designed this protein ourselves.
The idea for repeated sequences of amide group amino acids came from the following article:
"New ???Multicolor??? Polypeptide Diamagnetic Chemical Exchange Saturation Transfer (DIACEST) Contrast Agents for MRI", McMahon et al.
This part contains the DNA sequence of a protein of our own design. We first repeated the Arginine-Glycine amino acid pair 6 times to obtain a sequence of 12 amino acids. This sequence was then repeated a total of 36 times. Between these repeats no extra amino acids were added.
The idea behind this protein was to provide ourselves with a high concentration of amino acids with amide groups which we could then use to provide CEST contrast in an MRI.
false
false
_1435_
0
16850
9
Not in stock
false
We had designed the entire protein sequence from scratch, repeating the Arginine-Glycine amino acid pair a total number of 216 times. We randomised the codon usage for optimalization in E.coli.
false
Ardjan van der Linden
BBa_K1123018_sequence
1
cgcggacgcggtcgtggacgtggtcggggtagaggtcgcggtcgcgggcgtggccgtggccgtggccggggccgcggccgcggtcgcggacgtggacgtggtcgcggtcgtggtcgcggcaggggtcgtggccgcggtcgtggtaggggtcgcggtagaggccgcggtcgcggtcgcggtcgtggtcgcggtcgtggtcgaggacgcgggcgcggacgtggacgtggtcgtggtcgtggtcgtggccgcggacgtgggcgaggccgtggccgtggtcgcggccgtggtcggggtcgtggccgaggtcgcgggcgtggccgtggtcgtggtcgtggtcgcggccgcggtcgtggcagagggcgtggccgtgggcgtggtcgtggtcgtgggcgcgggcgtggccgcggtcgcggccgtggtcggggtcgtgga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z