BBa_K112704
1
S tag
<S tag!
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <S-tag! basic part K112704
Wobble PCR of dv011/dv012 (60 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112704
----------------------------------------
dv011 Forward Biobricking of <S-Tag!
cgataGAATTCatgAGATCTaaagaaaccgctgctgctaaattcgaacgcc
dv012 Reverse Biobricking of <S-Tag!
cgttaGGATCCttagctgtccatgtgctggcgttcgaatttagcagc
</pre>
This S-tag can be added after a protein part.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
true
false
_224_
0
2998
9
Discontinued
false
false
Bing Xia
BBa_K112709
1
BBa_K112709
b1006
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <b1006> basic part K112709
Wobble PCR of dv021/dv022 (66bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+66, L)
Product is pBca1256-K112709
-----------------------------------------------
dv021 Forward biobricking of b1006
cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg
dv022 Reverse biobricking of b1006
GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt
</pre>
The b1006 terminator.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
true
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112325
1
BBa_K112325
{< S-Tag!}{< b1006 >}
2008-10-28T12:00:00Z
2015-05-08T01:09:16Z
{BBa_K112704} and {BBa_K112709}
{<S-Tag!}{<b1006>}
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2999
9
Not in stock
false
<pre>
Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112325
</pre>
false
Aron Lau
component1994546
1
BBa_K112709
component1994544
1
BBa_K112704
component1994545
1
BBa_K112999
annotation1994546
1
BBa_K112709
range1994546
1
55
94
annotation1994544
1
BBa_K112704
range1994544
1
1
48
annotation1994545
1
BBa_K112999
range1994545
1
49
54
BBa_K112704_sequence
1
aaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaa
BBa_K112709_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112325_sequence
1
aaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaaggatctaaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112999_sequence
1
ggatct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z