BBa_K112999 1 scar BBb1 scar sequence 2008-10-20T11:00:00Z 2015-05-08T01:09:20Z This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]. false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112105 1 BBa_K112105 BamHI endonuclease without start or stop codons 2008-10-20T11:00:00Z 2015-05-08T01:09:14Z <pre> PCR mv005/mv007 on pBca9145-I716210 (670bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112104 </pre> BamHI endonuclease without start or stop codons false false _224_ 0 3023 9 Not in stock false This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. -------------------------------------- <pre> PCR mv005/mv007 on pBca9145-I716210 (670bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112104 ----------------------------------------------- mv005 Forward Biobricking of r.BamHI! ccataGAATTCatgAGATCTatggaagtagaaaaagagtttattactgatgaagc mv007 Reverse Biobricking of r.BamHI> cgttaGGATCCtttgttttcaactttatctttcc </pre> false Madhvi Venkatesh BBa_K112330 1 BBa_K112330 {rbs_phoApp>}{< r.BamHI>} 2008-10-28T12:00:00Z 2015-05-08T01:09:16Z K112614 AK and K112105 KC {rbs_phoApp>}{< r.BamHI>} ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 Not in stock false ==pBCa1601AC-K112330== <pre> Digest K112614 AK (BgIII, XhoI) Digest K112105 KC(BamHI, XhoI) Product is pBjh1601AC-K112330 </pre> false Aron Lau component1994564 1 BBa_K112105 component1994563 1 BBa_K112999 component1994562 1 BBa_K112614 annotation1994564 1 BBa_K112105 range1994564 1 88 723 annotation1994562 1 BBa_K112614 range1994562 1 1 81 annotation1994563 1 BBa_K112999 range1994563 1 82 87 BBa_K112614 1 BBa_K112614 rbs.PhoA pp> in BBb 2008-10-21T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of {rbs_PhoA>} basic part K112614 PCR sc010/sc013 on MG1655 (112 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 ------------------------------------------------------ sc010 Fwd biobricking of {rbs_prepro>} cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac sc013 Reverse biobricking of {prepro>} ccagtGGATCCggcttttgtcacaggggtaaac </pre> This is a phoA prepro with a native rbs and start codon, but without a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 3386 9 It's complicated false N/A false Sherine Cheung BBa_K112105_sequence 1 gaagtagaaaaagagtttattactgatgaagcaaaagaattactatctaaagataagttaattcaacaagcatacaatgaagttaaaacatctatttgttcacctatttggccagcaacctcaaaaaccttcacgattaacaacaccgaaaagaattgtaacggtgtagtaccaattaaagaactatgttacaccttacttgaagatacatataactggtatagagaaaaaccccttgatatacttaaacttgaaaagaaaaaaggtggtccgattgatgtttataaagagttcatagaaaacagtgaacttaaacgtgtaggtatggaatttgaaacaggaaatattagttctgcccaccgttcaatgaacaaacttctattaggattaaaacatggcgaaattgatttggctattatccttatgcctattaaacaattggcctattatcttacagatcgtgttaccaatttcgaggaattagaaccttattttgaacttactgaaggacaaccatttatttttattggatttaatgctgaggcttataattctaatgtccctttaattcccaaaggttctgacggtatgtcaaaacgctcaattaagaaatggaaagataaagttgaaaacaaa BBa_K112999_sequence 1 ggatct BBa_K112614_sequence 1 gtacatggagaaaataaaatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagcc BBa_K112330_sequence 1 gtacatggagaaaataaaatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagccggatctgaagtagaaaaagagtttattactgatgaagcaaaagaattactatctaaagataagttaattcaacaagcatacaatgaagttaaaacatctatttgttcacctatttggccagcaacctcaaaaaccttcacgattaacaacaccgaaaagaattgtaacggtgtagtaccaattaaagaactatgttacaccttacttgaagatacatataactggtatagagaaaaaccccttgatatacttaaacttgaaaagaaaaaaggtggtccgattgatgtttataaagagttcatagaaaacagtgaacttaaacgtgtaggtatggaatttgaaacaggaaatattagttctgcccaccgttcaatgaacaaacttctattaggattaaaacatggcgaaattgatttggctattatccttatgcctattaaacaattggcctattatcttacagatcgtgttaccaatttcgaggaattagaaccttattttgaacttactgaaggacaaccatttatttttattggatttaatgctgaggcttataattctaatgtccctttaattcccaaaggttctgacggtatgtcaaaacgctcaattaagaaatggaaagataaagttgaaaacaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z