BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112105
1
BBa_K112105
BamHI endonuclease without start or stop codons
2008-10-20T11:00:00Z
2015-05-08T01:09:14Z
<pre>
PCR mv005/mv007 on pBca9145-I716210 (670bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112104
</pre>
BamHI endonuclease without start or stop codons
false
false
_224_
0
3023
9
Not in stock
false
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
--------------------------------------
<pre>
PCR mv005/mv007 on pBca9145-I716210 (670bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112104
-----------------------------------------------
mv005 Forward Biobricking of r.BamHI!
ccataGAATTCatgAGATCTatggaagtagaaaaagagtttattactgatgaagc
mv007 Reverse Biobricking of r.BamHI>
cgttaGGATCCtttgttttcaactttatctttcc
</pre>
false
Madhvi Venkatesh
BBa_K112330
1
BBa_K112330
{rbs_phoApp>}{< r.BamHI>}
2008-10-28T12:00:00Z
2015-05-08T01:09:16Z
K112614 AK and K112105 KC
{rbs_phoApp>}{< r.BamHI>}
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2999
9
Not in stock
false
==pBCa1601AC-K112330==
<pre>
Digest K112614 AK (BgIII, XhoI)
Digest K112105 KC(BamHI, XhoI)
Product is pBjh1601AC-K112330
</pre>
false
Aron Lau
component1994562
1
BBa_K112614
component1994563
1
BBa_K112999
component1994564
1
BBa_K112105
annotation1994563
1
BBa_K112999
range1994563
1
82
87
annotation1994564
1
BBa_K112105
range1994564
1
88
723
annotation1994562
1
BBa_K112614
range1994562
1
1
81
BBa_K112614
1
BBa_K112614
rbs.PhoA pp> in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of {rbs_PhoA>} basic part K112614
PCR sc010/sc013 on MG1655 (112 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
sc013 Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac
</pre>
This is a phoA prepro with a native rbs and start codon, but without a stop codon. It is in BBb format.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
It's complicated
false
N/A
false
Sherine Cheung
BBa_K112105_sequence
1
gaagtagaaaaagagtttattactgatgaagcaaaagaattactatctaaagataagttaattcaacaagcatacaatgaagttaaaacatctatttgttcacctatttggccagcaacctcaaaaaccttcacgattaacaacaccgaaaagaattgtaacggtgtagtaccaattaaagaactatgttacaccttacttgaagatacatataactggtatagagaaaaaccccttgatatacttaaacttgaaaagaaaaaaggtggtccgattgatgtttataaagagttcatagaaaacagtgaacttaaacgtgtaggtatggaatttgaaacaggaaatattagttctgcccaccgttcaatgaacaaacttctattaggattaaaacatggcgaaattgatttggctattatccttatgcctattaaacaattggcctattatcttacagatcgtgttaccaatttcgaggaattagaaccttattttgaacttactgaaggacaaccatttatttttattggatttaatgctgaggcttataattctaatgtccctttaattcccaaaggttctgacggtatgtcaaaacgctcaattaagaaatggaaagataaagttgaaaacaaa
BBa_K112999_sequence
1
ggatct
BBa_K112614_sequence
1
gtacatggagaaaataaaatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagcc
BBa_K112330_sequence
1
gtacatggagaaaataaaatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagccggatctgaagtagaaaaagagtttattactgatgaagcaaaagaattactatctaaagataagttaattcaacaagcatacaatgaagttaaaacatctatttgttcacctatttggccagcaacctcaaaaaccttcacgattaacaacaccgaaaagaattgtaacggtgtagtaccaattaaagaactatgttacaccttacttgaagatacatataactggtatagagaaaaaccccttgatatacttaaacttgaaaagaaaaaaggtggtccgattgatgtttataaagagttcatagaaaacagtgaacttaaacgtgtaggtatggaatttgaaacaggaaatattagttctgcccaccgttcaatgaacaaacttctattaggattaaaacatggcgaaattgatttggctattatccttatgcctattaaacaattggcctattatcttacagatcgtgttaccaatttcgaggaattagaaccttattttgaacttactgaaggacaaccatttatttttattggatttaatgctgaggcttataattctaatgtccctttaattcccaaaggttctgacggtatgtcaaaacgctcaattaagaaatggaaagataaagttgaaaacaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z