BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112332
1
BBa_K112332
{rbs_lamB>} {< ihfA>}
2008-10-28T12:00:00Z
2015-05-08T01:09:16Z
BBa_K112615 and BBa_K112216
{rbs_lamB>} {< ihfA>}
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2999
9
Not in stock
false
<pre>
Digest (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112332
</pre>
false
Aron Lau
component1994570
1
BBa_K112216
component1994569
1
BBa_K112999
component1994568
1
BBa_K112615
annotation1994570
1
BBa_K112216
range1994570
1
101
394
annotation1994569
1
BBa_K112999
range1994569
1
95
100
annotation1994568
1
BBa_K112615
range1994568
1
1
94
BBa_K112615
1
BBa_K112615
rbs.LamB pp> in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of {rbs_LamB>} basic part K112615
PCR sc011/sc014 on MG1655 (126 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc011 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTcaatgactcaggagatagaatg
sc014 Reverse biobricking of {prepro>}
ccagtGGATCCagccattgcctgagcagac
</pre>
This is a lambB prepro with a native rbs and start codon, but without a stop codon. It is in BBb format.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
It's complicated
false
N/A
false
Sherine Cheung
BBa_K112216
1
BBa_K112216
{< ihfA >} The ihf alpha gene without start and stop codons, BBb format
2008-10-20T11:00:00Z
2015-05-08T01:09:15Z
MG1655
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2048
9
It's complicated
true
?
false
Molly Allen
BBa_K112615_sequence
1
caatgactcaggagatagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggct
BBa_K112332_sequence
1
caatgactcaggagatagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggctggatctgcgcttacaaaagctgaaatgtcagaatatctgtttgataagcttgggcttagcaagcgggatgccaaagaactggttgaactgtttttcgaagagatccgtcgcgctctggaaaacggcgaacaggtgaaactctctggttttggtaacttcgatctgcgtgataagaatcaacgcccgggacgtaacccgaaaacgggcgaggatattcccattacagcacggcgcgtggtgaccttcagacccgggcagaagttaaaaagccgggtcgaaaacgcttcgcccaaagacgag
BBa_K112999_sequence
1
ggatct
BBa_K112216_sequence
1
gcgcttacaaaagctgaaatgtcagaatatctgtttgataagcttgggcttagcaagcgggatgccaaagaactggttgaactgtttttcgaagagatccgtcgcgctctggaaaacggcgaacaggtgaaactctctggttttggtaacttcgatctgcgtgataagaatcaacgcccgggacgtaacccgaaaacgggcgaggatattcccattacagcacggcgcgtggtgaccttcagacccgggcagaagttaaaaagccgggtcgaaaacgcttcgcccaaagacgag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z