BBa_K112333
1
BBa_K112333
{rbs_phoApp>}{< ihfB>}
2008-10-28T12:00:00Z
2015-05-08T01:09:16Z
BBa_K112614 and BBa_K112222
{rbs_phoApp>}{< ihfB>}
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2999
9
Not in stock
false
<pre>
Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112333
</pre>
false
Aron Lau
component1994571
1
BBa_K112614
component1994572
1
BBa_K112999
component1994573
1
BBa_K112222
annotation1994573
1
BBa_K112222
range1994573
1
88
366
annotation1994572
1
BBa_K112999
range1994572
1
82
87
annotation1994571
1
BBa_K112614
range1994571
1
1
81
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112614
1
BBa_K112614
rbs.PhoA pp> in BBb
2008-10-21T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of {rbs_PhoA>} basic part K112614
PCR sc010/sc013 on MG1655 (112 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112605
------------------------------------------------------
sc010 Fwd biobricking of {rbs_prepro>}
cgataGAATTCatgAGATCTgtacatggagaaaataaaAtgaaacaaagcac
sc013 Reverse biobricking of {prepro>}
ccagtGGATCCggcttttgtcacaggggtaaac
</pre>
This is a phoA prepro with a native rbs and start codon, but without a stop codon. It is in BBb format.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3386
9
It's complicated
false
N/A
false
Sherine Cheung
BBa_K112222
1
BBa_K112222
{< ihfB >} The ihf beta gene without start or stop codons, BBb format
2008-10-20T11:00:00Z
2015-05-08T01:09:15Z
MG1655
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2048
9
It's complicated
true
?
false
Molly Allen
BBa_K112333_sequence
1
gtacatggagaaaataaaatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagccggatctaccaagtcagaattgatagaaagacttgccacccagcaatcgcacattcccgccaagacggttgaagatgcagtaaaagagatgctggagcatatggcctcgactcttgcgcagggcgagcgtattgaaatccgcggtttcggcagtttctctttgcactaccgcgcaccacgtaccggacgtaatccgaagactggcgataaagtagaactggaaggaaaatacgttcctcactttaaacctggtaaagaactgcgcgatcgcgccaatatttacggt
BBa_K112222_sequence
1
accaagtcagaattgatagaaagacttgccacccagcaatcgcacattcccgccaagacggttgaagatgcagtaaaagagatgctggagcatatggcctcgactcttgcgcagggcgagcgtattgaaatccgcggtttcggcagtttctctttgcactaccgcgcaccacgtaccggacgtaatccgaagactggcgataaagtagaactggaaggaaaatacgttcctcactttaaacctggtaaagaactgcgcgatcgcgccaatatttacggt
BBa_K112999_sequence
1
ggatct
BBa_K112614_sequence
1
gtacatggagaaaataaaatgaaacaaagcactattgcactggcactcttaccgttactgtttacccctgtgacaaaagcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z