BBa_K112324 1 BBa_K112324 {< phoA!}{< b1006 >} in BBb 2008-10-21T11:00:00Z 2015-05-08T01:09:16Z {<phoA!}{<b1006>} in BBb false false _224_ 0 2999 9 Not in stock false false Aron Lau component1983849 1 BBa_K112999 component1983850 1 BBa_K112709 component1983848 1 BBa_K112902 annotation1983850 1 BBa_K112709 range1983850 1 1377 1416 annotation1983848 1 BBa_K112902 range1983848 1 1 1354 annotation1983849 1 BBa_K112999 range1983849 1 1363 1368 BBa_K112709 1 BBa_K112709 b1006 2008-10-20T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of <b1006> basic part K112709 Wobble PCR of dv021/dv022 (66bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+66, L) Product is pBca1256-K112709 ----------------------------------------------- dv021 Forward biobricking of b1006 cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg dv022 Reverse biobricking of b1006 GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt </pre> The b1006 terminator. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false true _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112902 1 phoA < phoA ! 2008-10-21T11:00:00Z 2015-05-08T01:09:19Z Bca1126 phoA protein with no start codon and has stop codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 Not in stock false false Aron Lau BBa_K112999 1 scar BBb1 scar sequence 2008-10-20T11:00:00Z 2015-05-08T01:09:20Z This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]. false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112343 1 BBa_K112343 {rbs_lamB>} {< r.BglII>}{< phoA!}{< b1006>} 2008-10-28T12:00:00Z 2015-05-08T01:09:16Z BBa_K112331 and BBa_K112324 {rbs_lamB>} {< r.BglII>}{< phoA!}{< b1006>} ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 Not in stock false <pre> Digest (BgIII methylated) K112331 AC (BamHI/XhoI) Digest (BamHI methylated) K112324 CK (BgIII/XHoI) Product is pBjh1601AK-K112343 </pre> false Aron Lau component2225056 1 BBa_K112331 component2225061 1 BBa_K112324 component2225057 1 BBa_K112999 annotation2225061 1 BBa_K112324 range2225061 1 773 2188 annotation2225056 1 BBa_K112331 range2225056 1 1 766 annotation2225057 1 BBa_K112999 range2225057 1 767 772 BBa_K112108 1 BBa_K112108 BglII endonuclease without start or stop codons 2008-10-20T11:00:00Z 2015-05-08T01:09:14Z <pre> PCR mv012/mv011 on pBca9145-Bca1147 (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112108 </pre> BglII endonuclease without start or stop codons false false _224_ 0 3023 9 It's complicated true This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. ------------------------------------- <pre> PCR mv012/mv011 on pBca9145-Bca1147 (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112108 ----------------------------------------------- mv012 Forward Biobricking of <r.BglII> ccataGAATTCatgAGATCTaagattgatataacggactataacc mv011 Reverse Biobricking of r.BglII> cgttaGGATCCatatgtcacgattgttcctc </pre> false Madhvi Venkatesh BBa_K112615 1 BBa_K112615 rbs.LamB pp> in BBb 2008-10-21T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of {rbs_LamB>} basic part K112615 PCR sc011/sc014 on MG1655 (126 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 ------------------------------------------------------ sc011 Fwd biobricking of {rbs_prepro>} cgataGAATTCatgAGATCTcaatgactcaggagatagaatg sc014 Reverse biobricking of {prepro>} ccagtGGATCCagccattgcctgagcagac </pre> This is a lambB prepro with a native rbs and start codon, but without a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 3386 9 It's complicated false N/A false Sherine Cheung BBa_K112331 1 BBa_K112331 {rbs_lamB>} {< r.BglII>} 2008-10-28T12:00:00Z 2015-05-08T01:09:16Z BBa_K112615 and BBa_K112108 {rbs_lamB>} {< r.BglII>} ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 Not in stock false ==pBCa1601AC-K112331== <pre> Digest K112615 AK (BgIII, XhoI) Digest K112108 KC(BamHI, XhoI) Product is pBjh1601AC-K112331 </pre> false Aron Lau component1994567 1 BBa_K112108 component1994566 1 BBa_K112999 component1994565 1 BBa_K112615 annotation1994567 1 BBa_K112108 range1994567 1 101 766 annotation1994565 1 BBa_K112615 range1994565 1 1 94 annotation1994566 1 BBa_K112999 range1994566 1 95 100 BBa_K112709_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttta BBa_K112324_sequence 1 gacaccacaacaatatccgtactggataatcgtgcggcgcagggcgatattactacgcccggcggcgcgcgccgattaaccggcgatcaaacggcggcgctgcgtgaatcgctaaatgataaaccggcgaaaaatattattctgctgatcggcgacggaatgggagattctgaaataaccgccgcacgaaattatgccgaaggcgccggcggcttttttaaaggtattgatgccctgccgctgaccggacaatatacccactatgcgctgaataaaaaaaccggcaaaccagattacgtgaccgattccgccgcctcagccaccgcgtggtccacaggggtgaaaacctacaacggcgcgctgggggtcgatattcatgaaaaagatcatctgaccattctcgaaatggcgaaggccgcaggtctggctaccggcaacgtctcgaccgcggagttgcaggacgccaccccggcagcgctgattgcccatgtcacctcgcgcaaatgctatggcccgacggcgaccagcgaaaaatgtccgacaaacgcgctggaaaagggcggcaaaggctcgattactgaacagctcctgaacgcgcgggcggacgtcaccctgggcggcggcgcgaaaaccttcgccgagacggcaaccgccggcgagtggcaggggaaaacgctgcgtgagcaggcgcagatgcgcggctaccagttagtcggcgatgccgcctctttagcggcgatcgacgaagcgaatcaggacaaaccgctgctgggactgttcgcagaggggaatatgccggtgcgctgggaagggccgaaggcctcgtaccacggcaatattgataaacctgccgtgacctgtacgccaaatccgaaacgcaatgacgcgattcccacgctggcgcagatgaccgacaaagccatcgaactgttgagcaaaaatgagcggggcttctttttacaggtggaaggcgcgtctatcgataagcaggatcacgccgcgaacccgtgcggacagattggcgagacggtggatcttgatgaagccgtacagagggcgctggcctttgcgaagaaagacggcaatacgctggtgatcgttaccgccgatcatgctcatgccagccagatcgtggcgcccgaaacgaaagcgccggggctgacgcaggcgctgaacaccaaagatggcgcggtgatggtcatcagctacggcaactcggaagaagattcccaggagcataccggcagccagctgcgcatcgccgcttacgggccgcatgcggcgaacgtggtcgggctgaccgatcaaaccgatctgttctacaccatgaaagcggcgctgggcctgaagtaattactagagggatcttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttta BBa_K112902_sequence 1 gacaccacaacaatatccgtactggataatcgtgcggcgcagggcgatattactacgcccggcggcgcgcgccgattaaccggcgatcaaacggcggcgctgcgtgaatcgctaaatgataaaccggcgaaaaatattattctgctgatcggcgacggaatgggagattctgaaataaccgccgcacgaaattatgccgaaggcgccggcggcttttttaaaggtattgatgccctgccgctgaccggacaatatacccactatgcgctgaataaaaaaaccggcaaaccagattacgtgaccgattccgccgcctcagccaccgcgtggtccacaggggtgaaaacctacaacggcgcgctgggggtcgatattcatgaaaaagatcatctgaccattctcgaaatggcgaaggccgcaggtctggctaccggcaacgtctcgaccgcggagttgcaggacgccaccccggcagcgctgattgcccatgtcacctcgcgcaaatgctatggcccgacggcgaccagcgaaaaatgtccgacaaacgcgctggaaaagggcggcaaaggctcgattactgaacagctcctgaacgcgcgggcggacgtcaccctgggcggcggcgcgaaaaccttcgccgagacggcaaccgccggcgagtggcaggggaaaacgctgcgtgagcaggcgcagatgcgcggctaccagttagtcggcgatgccgcctctttagcggcgatcgacgaagcgaatcaggacaaaccgctgctgggactgttcgcagaggggaatatgccggtgcgctgggaagggccgaaggcctcgtaccacggcaatattgataaacctgccgtgacctgtacgccaaatccgaaacgcaatgacgcgattcccacgctggcgcagatgaccgacaaagccatcgaactgttgagcaaaaatgagcggggcttctttttacaggtggaaggcgcgtctatcgataagcaggatcacgccgcgaacccgtgcggacagattggcgagacggtggatcttgatgaagccgtacagagggcgctggcctttgcgaagaaagacggcaatacgctggtgatcgttaccgccgatcatgctcatgccagccagatcgtggcgcccgaaacgaaagcgccggggctgacgcaggcgctgaacaccaaagatggcgcggtgatggtcatcagctacggcaactcggaagaagattcccaggagcataccggcagccagctgcgcatcgccgcttacgggccgcatgcggcgaacgtggtcgggctgaccgatcaaaccgatctgttctacaccatgaaagcggcgctgggcctgaagtaat BBa_K112615_sequence 1 caatgactcaggagatagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggct BBa_K112343_sequence 1 caatgactcaggagatagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggctggatctaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatatggatctgacaccacaacaatatccgtactggataatcgtgcggcgcagggcgatattactacgcccggcggcgcgcgccgattaaccggcgatcaaacggcggcgctgcgtgaatcgctaaatgataaaccggcgaaaaatattattctgctgatcggcgacggaatgggagattctgaaataaccgccgcacgaaattatgccgaaggcgccggcggcttttttaaaggtattgatgccctgccgctgaccggacaatatacccactatgcgctgaataaaaaaaccggcaaaccagattacgtgaccgattccgccgcctcagccaccgcgtggtccacaggggtgaaaacctacaacggcgcgctgggggtcgatattcatgaaaaagatcatctgaccattctcgaaatggcgaaggccgcaggtctggctaccggcaacgtctcgaccgcggagttgcaggacgccaccccggcagcgctgattgcccatgtcacctcgcgcaaatgctatggcccgacggcgaccagcgaaaaatgtccgacaaacgcgctggaaaagggcggcaaaggctcgattactgaacagctcctgaacgcgcgggcggacgtcaccctgggcggcggcgcgaaaaccttcgccgagacggcaaccgccggcgagtggcaggggaaaacgctgcgtgagcaggcgcagatgcgcggctaccagttagtcggcgatgccgcctctttagcggcgatcgacgaagcgaatcaggacaaaccgctgctgggactgttcgcagaggggaatatgccggtgcgctgggaagggccgaaggcctcgtaccacggcaatattgataaacctgccgtgacctgtacgccaaatccgaaacgcaatgacgcgattcccacgctggcgcagatgaccgacaaagccatcgaactgttgagcaaaaatgagcggggcttctttttacaggtggaaggcgcgtctatcgataagcaggatcacgccgcgaacccgtgcggacagattggcgagacggtggatcttgatgaagccgtacagagggcgctggcctttgcgaagaaagacggcaatacgctggtgatcgttaccgccgatcatgctcatgccagccagatcgtggcgcccgaaacgaaagcgccggggctgacgcaggcgctgaacaccaaagatggcgcggtgatggtcatcagctacggcaactcggaagaagattcccaggagcataccggcagccagctgcgcatcgccgcttacgggccgcatgcggcgaacgtggtcgggctgaccgatcaaaccgatctgttctacaccatgaaagcggcgctgggcctgaagtaattactagagggatcttactagagaaaaaaaaaccccgcccctgacagggcggggtttttttta BBa_K112999_sequence 1 ggatct BBa_K112108_sequence 1 aagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatat BBa_K112331_sequence 1 caatgactcaggagatagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggctggatctaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z