BBa_K112704
1
S tag
<S tag!
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <S-tag! basic part K112704
Wobble PCR of dv011/dv012 (60 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112704
----------------------------------------
dv011 Forward Biobricking of <S-Tag!
cgataGAATTCatgAGATCTaaagaaaccgctgctgctaaattcgaacgcc
dv012 Reverse Biobricking of <S-Tag!
cgttaGGATCCttagctgtccatgtgctggcgttcgaatttagcagc
</pre>
This S-tag can be added after a protein part.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
true
false
_224_
0
2998
9
Discontinued
false
false
Bing Xia
BBa_K112709
1
BBa_K112709
b1006
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <b1006> basic part K112709
Wobble PCR of dv021/dv022 (66bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+66, L)
Product is pBca1256-K112709
-----------------------------------------------
dv021 Forward biobricking of b1006
cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg
dv022 Reverse biobricking of b1006
GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt
</pre>
The b1006 terminator.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
true
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112102
1
cre
Cre enzyme without start or stop codons
2008-10-20T11:00:00Z
2015-05-08T01:09:13Z
<pre>
PCR mv001/mv004 on pBca9145-Bca1010 (1057bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112102
</pre>
Cre enzyme without start or stop codons
false
false
_224_
0
3023
9
It's complicated
true
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
----------------------------------------
<pre>
PCR mv001/mv004 on pBca9145-Bca1010 (1057bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112102
-----------------------------------------------
mv001 Forward Biobricking of <Cre!
ccataGAATTCatgAGATCTtccaatttactgaccgtacacc
mv004 Reverse Biobricking of Cre>
cgttaGGATCCatcgccatcttccagcaggcg
</pre>
false
Madhvi Venkatesh
BBa_K112348
1
BBa_K112348
{rbs_ompT>} {< Cre>}{< S-Tag!}{< b1006>}
2008-10-28T12:00:00Z
2015-05-08T01:09:16Z
BBa_K112328 and BBa_K112325
{rbs_ompT>} {< Cre>}{< S-Tag!}{< b1006>}
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2999
9
Not in stock
false
<pre>
Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112348
</pre>
false
Aron Lau
component1994635
1
BBa_K112709
component1994633
1
BBa_K112704
component1994630
1
BBa_K112999
component1994631
1
BBa_K112102
component1994629
1
BBa_K112227
component1994632
1
BBa_K112999
component1994634
1
BBa_K112999
annotation1994634
1
BBa_K112999
range1994634
1
1170
1175
annotation1994630
1
BBa_K112999
range1994630
1
84
89
annotation1994632
1
BBa_K112999
range1994632
1
1116
1121
annotation1994629
1
BBa_K112227
range1994629
1
1
83
annotation1994635
1
BBa_K112709
range1994635
1
1176
1215
annotation1994631
1
BBa_K112102
range1994631
1
90
1115
annotation1994633
1
BBa_K112704
range1994633
1
1122
1169
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112227
1
ompT
{rbs.ompT} The ompT gene with native rbs, BBb format
2008-10-20T11:00:00Z
2015-05-08T01:09:15Z
Synthetic
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2048
9
It's complicated
false
?
false
Molly Allen
BBa_K112704_sequence
1
aaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaa
BBa_K112709_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112102_sequence
1
tccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggattcgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgat
BBa_K112999_sequence
1
ggatct
BBa_K112348_sequence
1
aagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccctatcgcgatcagctcttttgcgtcgacgggatcttccaatttactgaccgtacaccaaaatttgcctgcattaccggtcgatgcaacgagtgatgaggttcgcaagaacctgatggacatgttcagggatcgccaggcgttttctgagcatacctggaaaatgcttctgtccgtttgccggtcgtgggcggcatggtgcaagttgaataaccggaaatggtttcccgcagaacctgaagatgttcgcgattatcttctatatcttcaggcgcgcggtctggcagtaaaaactatccagcaacatttgggccagctaaacatgcttcatcgtcggtccgggctgccacgaccaagtgacagcaatgctgtttcactggttatgcggcggattcgaaaagaaaacgttgatgccggtgaacgtgcaaaacaggctctagcgttcgaacgcactgatttcgaccaggttcgttcactcatggaaaatagcgatcgctgccaggatatacgtaatctggcatttctggggattgcttataacaccctgttacgtatagccgaaattgccaggatcagggttaaagatatctcacgtactgacggtgggagaatgttaatccatattggcagaacgaaaacgctggttagcaccgcaggtgtagagaaggcacttagcctgggggtaactaaactggtcgagcgatggatttccgtctctggtgtagctgatgatccgaataactacctgttttgccgggtcagaaaaaatggtgttgccgcgccatctgccaccagccagctatcaactcgcgccctggaagggatttttgaagcaactcatcgattgatttacggcgctaaggatgactctggtcagagatacctggcctggtctggacacagtgcccgtgtcggagccgcgcgagatatggcccgcgctggagtttcaataccggagatcatgcaagctggtggctggaccaatgtaaatattgtcatgaactatatccgtaacctggatagtgaaacaggggcaatggtgcgcctgctggaagatggcgatggatctaaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaaggatctaaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112227_sequence
1
aagaaggagatatacatatgcgggcgaaactcctaggaatagtcctgacaacccctatcgcgatcagctcttttgcgtcgacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z