BBa_K112704
1
S tag
<S tag!
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <S-tag! basic part K112704
Wobble PCR of dv011/dv012 (60 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112704
----------------------------------------
dv011 Forward Biobricking of <S-Tag!
cgataGAATTCatgAGATCTaaagaaaccgctgctgctaaattcgaacgcc
dv012 Reverse Biobricking of <S-Tag!
cgttaGGATCCttagctgtccatgtgctggcgttcgaatttagcagc
</pre>
This S-tag can be added after a protein part.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
true
false
_224_
0
2998
9
Discontinued
false
false
Bing Xia
BBa_K112709
1
BBa_K112709
b1006
2008-10-20T11:00:00Z
2015-05-08T01:09:18Z
<pre>
Construction of <b1006> basic part K112709
Wobble PCR of dv021/dv022 (66bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+66, L)
Product is pBca1256-K112709
-----------------------------------------------
dv021 Forward biobricking of b1006
cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg
dv022 Reverse biobricking of b1006
GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt
</pre>
The b1006 terminator.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
true
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112230
1
rbs.pelB
{rbs.pelB} The pelB leader with native rbs; assembly standard 21
2008-10-20T11:00:00Z
2015-05-08T01:09:15Z
Synthetic
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
true
_224_
0
2048
9
It's complicated
false
?
false
Molly Allen
BBa_K112113
1
BBa_K112113
Ligase without start or stop codons
2008-10-20T11:00:00Z
2015-05-08T01:09:14Z
<pre>
PCR mv022/mv023 on pBca1256-K112109 (1489bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112113
-----------------------------------------------
mv022 Forward Biobricking of <ligase!
ccataGAATTCatgAGATCTattcttaaaattctgaacg
mv023 Reverse Biobricking of ligase>
cgttaGGATCCtagaccagttacctcatg
</pre>
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
It's complicated
true
false
Bing Xia
BBa_K112349
1
BBa_K112349
{rbs_pelB>} {< ligase>}{< S-Tag!}{< b1006>}
2008-10-28T12:00:00Z
2015-05-08T01:09:16Z
BBa_K112329 and BBa_K112325
{rbs_pelB>} {< ligase>}{< S-Tag!}{< b1006>}
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2999
9
Not in stock
false
<pre>
Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112349
</pre>
false
Aron Lau
component1994637
1
BBa_K112999
component1994636
1
BBa_K112230
component1994641
1
BBa_K112999
component1994639
1
BBa_K112999
component1994642
1
BBa_K112709
component1994638
1
BBa_K112113
component1994640
1
BBa_K112704
annotation1994636
1
BBa_K112230
range1994636
1
1
85
annotation1994637
1
BBa_K112999
range1994637
1
86
91
annotation1994640
1
BBa_K112704
range1994640
1
1556
1603
annotation1994638
1
BBa_K112113
range1994638
1
92
1549
annotation1994639
1
BBa_K112999
range1994639
1
1550
1555
annotation1994641
1
BBa_K112999
range1994641
1
1604
1609
annotation1994642
1
BBa_K112709
range1994642
1
1610
1649
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112704_sequence
1
aaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaa
BBa_K112709_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112113_sequence
1
attcttaaaattctgaacgaaatagcatctattggttcaactaaacagaagcaagcaattcttgaaaagaataaagataatgaattgcttaaacgagtatatcgtctgacttattctcgtgggttacagtattatatcaagaaatggcctaaacctggtattgctacccagagttttggaatgttgactcttaccgatatgcttgacttcattgagttcacattagctactcggaaattgactggaaatgcagcaattgaggaattaactggatatatcaccgatggtaaaaaagatgatgttgaagttttgcgtcgagtgatgatgcgagaccttgaatgtggtgcttcagtatctattgcaaacaaagtttggccaggtttaattcctgaacaacctcaaatgctcgcaagttcttatgatgaaaaaggcattaataagaatatcaaatttccagcctttgctcagttaaaagctgatggagctcggtgttttgctgaagttagaggtgatgaattagatgatgttcgtcttttatcacgagctggtaatgaatatctaggattagaccttcttaaggaagagttaattaaaatgaccgctgaagcccgccagattcatccagaaggtgtgttgattgatggcgaattggtataccatgagcaagttaaaaaggagccagaaggcctagattttctttttgatgcttatcctgaaaacagtaaagctaaagagttcgccgaagtagctgaatcacgtactgcttctaatggaatcgccaataaatctttaaagggaaccatttctgaaaaagaagcacaatgcatgaagtttcaggtctgggattatgtcccgttggtagaaatatacagtcttcctgcatttcgtttgaaatatgatgtacgtttttctaaactagaacaaatgacatctggatatgataaagtaattttaattgaaaaccaggtagtaaataacctagatgaagctaaggtaatttataaaaagtatattgaccaaggtcttgaaggtattattctcaaaaatatcgatggattatgggaaaatgctcgttcaaaaaatctttataaatttaaagaagtaattgatgttgatttaaaaattgtaggaatttatcctcaccgtaaagaccctactaaagcgggtggatttattcttgagtcagagtgtggaaaaattaaggtaaatgctggttcaggcttaaaagataaagccggtgtaaaatcgcatgaacttgaccgtactcgcattatggaaaaccaaaattattatattggaaaaattctagagtgcgaatgcaacggttggttaaaatctgatggccgcactgattacgttaaattatttcttccgattgcgattcgtttacgtgaagataaaactaaagctaatacattcgaagatgtatttggtgattttcatgaggtaactggtcta
BBa_K112230_sequence
1
ttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
BBa_K112349_sequence
1
ttaagaaggagatatacatatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccggatctattcttaaaattctgaacgaaatagcatctattggttcaactaaacagaagcaagcaattcttgaaaagaataaagataatgaattgcttaaacgagtatatcgtctgacttattctcgtgggttacagtattatatcaagaaatggcctaaacctggtattgctacccagagttttggaatgttgactcttaccgatatgcttgacttcattgagttcacattagctactcggaaattgactggaaatgcagcaattgaggaattaactggatatatcaccgatggtaaaaaagatgatgttgaagttttgcgtcgagtgatgatgcgagaccttgaatgtggtgcttcagtatctattgcaaacaaagtttggccaggtttaattcctgaacaacctcaaatgctcgcaagttcttatgatgaaaaaggcattaataagaatatcaaatttccagcctttgctcagttaaaagctgatggagctcggtgttttgctgaagttagaggtgatgaattagatgatgttcgtcttttatcacgagctggtaatgaatatctaggattagaccttcttaaggaagagttaattaaaatgaccgctgaagcccgccagattcatccagaaggtgtgttgattgatggcgaattggtataccatgagcaagttaaaaaggagccagaaggcctagattttctttttgatgcttatcctgaaaacagtaaagctaaagagttcgccgaagtagctgaatcacgtactgcttctaatggaatcgccaataaatctttaaagggaaccatttctgaaaaagaagcacaatgcatgaagtttcaggtctgggattatgtcccgttggtagaaatatacagtcttcctgcatttcgtttgaaatatgatgtacgtttttctaaactagaacaaatgacatctggatatgataaagtaattttaattgaaaaccaggtagtaaataacctagatgaagctaaggtaatttataaaaagtatattgaccaaggtcttgaaggtattattctcaaaaatatcgatggattatgggaaaatgctcgttcaaaaaatctttataaatttaaagaagtaattgatgttgatttaaaaattgtaggaatttatcctcaccgtaaagaccctactaaagcgggtggatttattcttgagtcagagtgtggaaaaattaaggtaaatgctggttcaggcttaaaagataaagccggtgtaaaatcgcatgaacttgaccgtactcgcattatggaaaaccaaaattattatattggaaaaattctagagtgcgaatgcaacggttggttaaaatctgatggccgcactgattacgttaaattatttcttccgattgcgattcgtttacgtgaagataaaactaaagctaatacattcgaagatgtatttggtgattttcatgaggtaactggtctaggatctaaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaaggatctaaaaaaaaaccccgcccctgacagggcggggtttttttta
BBa_K112999_sequence
1
ggatct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z