BBa_K112704 1 S tag <S tag! 2008-10-20T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of <S-tag! basic part K112704 Wobble PCR of dv011/dv012 (60 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112704 ---------------------------------------- dv011 Forward Biobricking of <S-Tag! cgataGAATTCatgAGATCTaaagaaaccgctgctgctaaattcgaacgcc dv012 Reverse Biobricking of <S-Tag! cgttaGGATCCttagctgtccatgtgctggcgttcgaatttagcagc </pre> This S-tag can be added after a protein part. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] true false _224_ 0 2998 9 Discontinued false false Bing Xia BBa_K112709 1 BBa_K112709 b1006 2008-10-20T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of <b1006> basic part K112709 Wobble PCR of dv021/dv022 (66bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+66, L) Product is pBca1256-K112709 ----------------------------------------------- dv021 Forward biobricking of b1006 cgataGAATTCatgAGATCTaaaaaaaaaccccgcccctgacagggcgg dv022 Reverse biobricking of b1006 GGATCCtaaaaaaaaccccgccctgtcaggggcggggtttttt </pre> The b1006 terminator. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false true _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112900 1 BBa_K112900 Pbad 2008-10-20T11:00:00Z 2015-05-08T01:09:19Z Arabinose induced promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112999 1 scar BBb1 scar sequence 2008-10-20T11:00:00Z 2015-05-08T01:09:20Z This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]. false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112108 1 BBa_K112108 BglII endonuclease without start or stop codons 2008-10-20T11:00:00Z 2015-05-08T01:09:14Z <pre> PCR mv012/mv011 on pBca9145-Bca1147 (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112108 </pre> BglII endonuclease without start or stop codons false false _224_ 0 3023 9 It's complicated true This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. ------------------------------------- <pre> PCR mv012/mv011 on pBca9145-Bca1147 (697bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112108 ----------------------------------------------- mv012 Forward Biobricking of <r.BglII> ccataGAATTCatgAGATCTaagattgatataacggactataacc mv011 Reverse Biobricking of r.BglII> cgttaGGATCCatatgtcacgattgttcctc </pre> false Madhvi Venkatesh BBa_K112615 1 BBa_K112615 rbs.LamB pp> in BBb 2008-10-21T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of {rbs_LamB>} basic part K112615 PCR sc011/sc014 on MG1655 (126 bp, EcoRI/BamHI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112605 ------------------------------------------------------ sc011 Fwd biobricking of {rbs_prepro>} cgataGAATTCatgAGATCTcaatgactcaggagatagaatg sc014 Reverse biobricking of {prepro>} ccagtGGATCCagccattgcctgagcagac </pre> This is a lambB prepro with a native rbs and start codon, but without a stop codon. It is in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 3386 9 It's complicated false N/A false Sherine Cheung BBa_K112371 1 BBa_K112371 {Pbad}{rbs_lamB>} {< r.BglII>}{< S-Tag!}{< b1006>} 2008-10-28T12:00:00Z 2015-05-08T01:09:17Z BBa_K112900 and BBa_K112351 {Pbad}{rbs_lamB>} {< r.BglII>}{< S-Tag!}{< b1006>} ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 It's complicated false <pre> Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK (BamHI/BgIII/XHoI) Product is pBjh1601CK-K112371 </pre> false Aron Lau component1994804 1 BBa_K112709 component1994803 1 BBa_K112999 component1994802 1 BBa_K112704 component1994796 1 BBa_K112900 component1994800 1 BBa_K112108 component1994797 1 BBa_K112999 component1994801 1 BBa_K112999 component1994799 1 BBa_K112999 component1994798 1 BBa_K112615 annotation1994798 1 BBa_K112615 range1994798 1 1232 1325 annotation1994803 1 BBa_K112999 range1994803 1 2052 2057 annotation1994799 1 BBa_K112999 range1994799 1 1326 1331 annotation1994800 1 BBa_K112108 range1994800 1 1332 1997 annotation1994801 1 BBa_K112999 range1994801 1 1998 2003 annotation1994804 1 BBa_K112709 range1994804 1 2058 2097 annotation1994797 1 BBa_K112999 range1994797 1 1226 1231 annotation1994802 1 BBa_K112704 range1994802 1 2004 2051 annotation1994796 1 BBa_K112900 range1994796 1 1 1225 BBa_K112704_sequence 1 aaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaa BBa_K112709_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttta BBa_K112615_sequence 1 caatgactcaggagatagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggct BBa_K112371_sequence 1 aggattcttagctttcgctaggatgatttctggaattcatgagatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtctttactggctcttctcgctaccaacggtaaccccgcttattaaaagcattctgtacaagcgggacaaagcatgacaaaaacgcgtaacaagtgtcttatatcacggcgaaagttcaatggatatttgcacgctcactgcatgctagcattttatcgataagattaccgatcttacctgaagctggatctcaatgactcaggagatagaatgatgattactctgcgcaaacttcctctggcggttgccgtcgcagcgggcgtaatgtctgctcaggcaatggctggatctaagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatatggatctaaagaaaccgctgctgctaaattcgaacgccagcacatggacagctaaggatctaaaaaaaaaccccgcccctgacagggcggggtttttttta BBa_K112999_sequence 1 ggatct BBa_K112108_sequence 1 aagattgatataacggactataaccatgctgatgaaatactaaatcctcaattatggaaagaaattgaagaaacgctattaaaaatgccattacacgttaaagcatcagatcaagccagcaaagttggcagtttaatttttgatcctgtcggtacaaatcaatacataaaagatgagttggtaccaaaacattggaaaaataatatccctatacctaaacgatttgacttcctaggtactgacatagattttggtaaaagagatacgctagttgaagttcagttttctaattacccatttctgctcaataatacggtacgttcagaactgtttcataaaagtaacatggacattgatgaagaaggaatgaaagtagcgatcattattactaaagggcatatgtttcccgcttctaacagttcattatattatgaacaagctcaaaatcaacttaactccttagctgaatataacgtttttgatgtacctataagattagtagggttaatagaagactttgaaactgatattgatattgtttcaactacatatgcggacaaacgctattcaaggacaataacaaaaagagataccgttaaaggtaaagtgattgataccaacacgccgaataccagacgtcggaaaagaggaacaatcgtgacatat BBa_K112900_sequence 1 aggattcttagctttcgctaggatgatttctggaattcatgagatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtctttactggctcttctcgctaccaacggtaaccccgcttattaaaagcattctgtacaagcgggacaaagcatgacaaaaacgcgtaacaagtgtcttatatcacggcgaaagttcaatggatatttgcacgctcactgcatgctagcattttatcgataagattaccgatcttacctgaagct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z