BBa_K112401
1
BBa_K112401
Promoter for recA gene - SOS and Ultrasound Sensitive
2008-10-20T11:00:00Z
2015-05-08T01:09:17Z
Cloned from E. coli MG1655 genome.
According to Vollmer, et. al in their 1998 Applied and Enviormental Microbiology paper, "Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubble", the promoter for the recA gene, a known SOS response gene, is upregulated 4 fold when cells are exposed to high frequency ultrasound and microbubble cavitation.
false
false
_224_
0
3241
9
It's complicated
false
We did not see the desired response from ultrasound exposure that we expected from this promoter. However, we did not induce the same acoustic cavitation that Vollmer et. al. used to elicit a response from this promoter.
false
Christina Brown
BBa_K112409
1
BBa_K112409
RecA reporter GFP construct
2008-10-30T12:00:00Z
2015-05-08T01:09:17Z
RecA cloned from E. coli genome, GFP from JC anderson lab at UC Berkeley
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3241
9
It's complicated
false
none
false
Christina Brown
component1999007
1
BBa_K112999
component1999008
1
BBa_K112416
component1999006
1
BBa_K112401
annotation1999008
1
BBa_K112416
range1999008
1
309
1094
annotation1999007
1
BBa_K112999
range1999007
1
295
300
annotation1999006
1
BBa_K112401
range1999006
1
1
286
BBa_K112999
1
scar
BBb1 scar sequence
2008-10-20T11:00:00Z
2015-05-08T01:09:20Z
This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page].
false
false
_224_
0
2998
9
Not in stock
false
false
Bing Xia
BBa_K112416
1
BBa_K112416
GFP reporter
2008-10-30T12:00:00Z
2015-05-08T01:09:17Z
from JC Anderson lab at UC Berkeley
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
3241
9
Not in stock
false
none
false
Christina Brown
BBa_K112416_sequence
1
aaagattaactttataaggaggaaaaacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactataagacacgtgctgaagtcaagtttgaaggtgatacacttgttaatagaatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaagttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtccaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgcagcaaacgacgaaaactacgctttagtagcttaa
BBa_K112401_sequence
1
agagaagcctgtcggcaccgtctggtttgcttttgccactgcccgcggtgaaggcattacccggcgggaatgcttcagcggcgaccgtgatgcggtgcgtcgtcaggctactgcgtatgcattgcagaccttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactggg
BBa_K112999_sequence
1
ggatct
BBa_K112409_sequence
1
agagaagcctgtcggcaccgtctggtttgcttttgccactgcccgcggtgaaggcattacccggcgggaatgcttcagcggcgaccgtgatgcggtgcgtcgtcaggctactgcgtatgcattgcagaccttgtggcaacaatttctacaaaacacttgatactgtatgagcatacagtataattgcttcaacagaacatattgactatccggtattacccggcatgacaggagtaaaaatggctatcgacgaaaacaaacagaaagcgttggcggcagcactgggtactagagggatcttactagagaaagattaactttataaggaggaaaaacatatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttgacttatggtgttcaatgcttttcaagatacccagatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactataagacacgtgctgaagtcaagtttgaaggtgatacacttgttaatagaatcgagttaaagggtattgattttaaagaagatggaaacattcttggacacaagttggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtccaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgcagcaaacgacgaaaactacgctttagtagcttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z