##gff-version 3
##sequence-region BBa_K1124100 1 9068
BBa_K1124100	.	engineered_region	58	759	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_K1124100	.	CDS	75	734	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124100	.	tag	695	728	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1124100	.	engineered_tag	735	759	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124100	.	CDS	78	694	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124100	.	ribosome_entry_site	58	68	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124100	.	ribosome_entry_site	58	68	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124100	.	engineered_region	58	68	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124100	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124100	.	promoter	2051	2099	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1124100	.	engineered_region	2051	2099	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1124100	.	promoter	2065	2070	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1124100	.	non_covalent_binding_site	2075	2091	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1124100	.	non_covalent_binding_site	2051	2067	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1124100	.	promoter	2088	2093	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1124100	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1124100	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1124100	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1124100	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1124100	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1124100	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1124100	.	engineered_region	2356	8144	.	+	0	ID=BBa_K1124101;Name=BBa_K1124101
BBa_K1124100	.	engineered_region	3092	3856	.	+	0	ID=BBa_K773003;Name=BBa_K773003;Parent=BBa_K1124101
BBa_K1124100	.	tag	3821	3850	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124100	.	start_codon	3110	3112	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124100	.	stop_codon	3851	3853	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124100	.	stop_codon	3854	3856	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124100	.	ribosome_entry_site	3092	3103	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124100	.	CDS	3110	3856	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124100	.	engineered_region	2764	3003	.	+	0	ID=BBa_K1124114;Name=BBa_K1124114;Parent=BBa_K1124101
BBa_K1124100	.	terminator	2875	3003	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124114
BBa_K1124100	.	terminator	2963	3003	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	2990	3003	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	2996	2996	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	2970	2989	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	2963	3003	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	2875	2954	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	2886	2929	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	2875	2954	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	mature_transcript_region	2764	2866	.	+	0	ID=BBa_K1124007;Name=BBa_K1124007;Parent=BBa_K1124114
BBa_K1124100	.	sequence_feature	2764	2787	.	+	0	ID=annotation2339035;Name=Target-binding sequence;Parent=BBa_K1124007
BBa_K1124100	.	sequence_feature	2788	2866	.	+	0	ID=annotation2339034;Name=MicC scaffold;Parent=BBa_K1124007
BBa_K1124100	.	promoter	7209	7243	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1124101
BBa_K1124100	.	ribosome_entry_site	6265	6276	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124101
BBa_K1124100	.	conserved_region	6269	6272	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124100	.	promoter	3012	3083	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K1124101
BBa_K1124100	.	promoter	3053	3058	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124100	.	start_codon	3064	3064	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124100	.	non_covalent_binding_site	3012	3031	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124100	.	non_covalent_binding_site	3065	3083	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124100	.	promoter	3031	3036	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124100	.	engineered_region	3012	3064	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124100	.	CDS	6283	7038	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K1124101
BBa_K1124100	.	engineered_tag	7039	7063	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1124100	.	engineered_region	6283	6284	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1124100	.	CDS	6283	7032	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1124100	.	point_mutation	6456	6456	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1124100	.	engineered_region	6283	7038	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1124100	.	point_mutation	6774	6774	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1124100	.	engineered_region	4534	5235	.	+	0	ID=BBa_S0107;Name=BBa_S0107;Parent=BBa_K1124101
BBa_K1124100	.	CDS	4551	5210	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124100	.	tag	5171	5204	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1124100	.	engineered_tag	5211	5235	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124100	.	CDS	4554	5170	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124100	.	ribosome_entry_site	4534	4544	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124100	.	ribosome_entry_site	4534	4544	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124100	.	engineered_region	4534	4544	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124100	.	ribosome_entry_site	4540	4543	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124100	.	engineered_region	7252	8144	.	+	0	ID=BBa_K1124110;Name=BBa_K1124110;Parent=BBa_K1124101
BBa_K1124100	.	ribosome_entry_site	7252	7263	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124110
BBa_K1124100	.	conserved_region	7256	7259	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124100	.	engineered_region	7270	8007	.	+	0	ID=BBa_K1124000;Name=BBa_K1124000;Parent=BBa_K1124110
BBa_K1124100	.	ribosome_entry_site	7270	7968	.	+	0	ID=annotation2338833;Name=amilGFP;Parent=BBa_K1124000
BBa_K1124100	.	stop_codon	8002	8004	.	+	0	ID=annotation2338831;Name=stop;Parent=BBa_K1124000
BBa_K1124100	.	tag	7969	8001	.	+	0	ID=annotation2338832;Name=LVA degradation tag;Parent=BBa_K1124000
BBa_K1124100	.	stop_codon	8005	8007	.	+	0	ID=annotation2338830;Name=stop;Parent=BBa_K1124000
BBa_K1124100	.	terminator	8016	8144	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124110
BBa_K1124100	.	terminator	8104	8144	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	8131	8144	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	8137	8137	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	8111	8130	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	8104	8144	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	8016	8095	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	8027	8070	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	8016	8095	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	promoter	6222	6256	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1124101
BBa_K1124100	.	promoter	2684	2755	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K1124101
BBa_K1124100	.	promoter	2725	2730	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124100	.	start_codon	2736	2736	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124100	.	non_covalent_binding_site	2684	2703	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124100	.	non_covalent_binding_site	2737	2755	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124100	.	promoter	2703	2708	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124100	.	engineered_region	2684	2736	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124100	.	engineered_region	5244	6213	.	+	0	ID=BBa_K516022;Name=BBa_K516022;Parent=BBa_K1124101
BBa_K1124100	.	CDS	5263	6051	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124100	.	engineered_region	5263	6051	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124100	.	tag	6013	6045	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124100	.	engineered_tag	6052	6076	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124100	.	CDS	5263	6012	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124100	.	start_codon	5263	5265	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124100	.	stop_codon	6046	6051	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124100	.	ribosome_entry_site	5244	5256	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124100	.	engineered_region	5244	5256	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124100	.	ribosome_entry_site	5244	5256	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124100	.	ribosome_entry_site	5250	5253	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124100	.	terminator	6085	6213	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124100	.	terminator	6173	6213	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	6200	6213	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	6206	6206	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	6180	6199	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	6173	6213	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	6085	6164	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	6096	6139	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	6085	6164	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	3865	4525	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K1124101
BBa_K1124100	.	CDS	3883	4500	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1124100	.	engineered_region	3883	4500	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1124100	.	CDS	3883	4461	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1124100	.	tag	4462	4493	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1124100	.	engineered_tag	4501	4525	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1124100	.	ribosome_entry_site	3865	3876	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1124100	.	conserved_region	3869	3872	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124100	.	promoter	2356	2427	.	+	0	ID=BBa_K176000;Name=BBa_K176000;Parent=BBa_K1124101
BBa_K1124100	.	promoter	2397	2402	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124100	.	start_codon	2408	2408	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124100	.	non_covalent_binding_site	2356	2375	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124100	.	non_covalent_binding_site	2409	2427	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124100	.	promoter	2375	2380	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124100	.	engineered_region	2356	2408	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124100	.	terminator	7072	7200	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124101
BBa_K1124100	.	terminator	7160	7200	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	7187	7200	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	7193	7193	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	7167	7186	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	7160	7200	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	7072	7151	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	7083	7126	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	7072	7151	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	2436	2675	.	+	0	ID=BBa_K1124113;Name=BBa_K1124113;Parent=BBa_K1124101
BBa_K1124100	.	mature_transcript_region	2436	2538	.	+	0	ID=BBa_K1124006;Name=BBa_K1124006;Parent=BBa_K1124113
BBa_K1124100	.	sequence_feature	2436	2459	.	+	0	ID=annotation2339033;Name=Target-binding sequence;Parent=BBa_K1124006
BBa_K1124100	.	sequence_feature	2460	2538	.	+	0	ID=annotation2339032;Name=MicC scaffold;Parent=BBa_K1124006
BBa_K1124100	.	terminator	2547	2675	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124113
BBa_K1124100	.	terminator	2635	2675	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	2662	2675	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	2668	2668	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	2642	2661	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	2635	2675	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	2547	2626	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	2558	2601	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	2547	2626	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	8196	9068	.	+	0	ID=BBa_K1124118;Name=BBa_K1124118
BBa_K1124100	.	ribosome_entry_site	8196	8207	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124118
BBa_K1124100	.	conserved_region	8200	8203	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124100	.	CDS	8214	8931	.	+	0	ID=BBa_K1124003;Name=BBa_K1124003;Parent=BBa_K1124118
BBa_K1124100	.	stop_codon	8925	8927	.	+	0	ID=annotation2340390;Name=stop;Parent=BBa_K1124003
BBa_K1124100	.	stop_codon	8928	8930	.	+	0	ID=annotation2340391;Name=stop;Parent=BBa_K1124003
BBa_K1124100	.	CDS	8214	8924	.	+	0	ID=annotation2340392;Name=lambda cI repressor (E234K);Parent=BBa_K1124003
BBa_K1124100	.	point_mutation	8913	8913	.	+	0	ID=annotation2340454;Name=E234K (point mutation G to A);Parent=BBa_K1124003
BBa_K1124100	.	terminator	8940	9068	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124118
BBa_K1124100	.	terminator	9028	9068	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	9055	9068	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	9061	9061	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	9035	9054	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	9028	9068	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	8940	9019	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	8951	8994	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	8940	9019	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	promoter	8153	8187	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1124100	.	engineered_region	768	1737	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124100	.	CDS	787	1575	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124100	.	engineered_region	787	1575	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124100	.	tag	1537	1569	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124100	.	engineered_tag	1576	1600	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124100	.	CDS	787	1536	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124100	.	start_codon	787	789	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124100	.	stop_codon	1570	1575	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124100	.	ribosome_entry_site	768	780	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124100	.	engineered_region	768	780	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124100	.	ribosome_entry_site	768	780	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124100	.	ribosome_entry_site	774	777	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124100	.	terminator	1609	1737	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124100	.	terminator	1697	1737	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	1724	1737	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	1730	1730	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	1704	1723	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	1697	1737	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	1609	1688	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	1620	1663	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	1609	1688	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	promoter	1746	1794	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1124100	.	engineered_region	1746	1794	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1124100	.	promoter	1760	1765	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1124100	.	non_covalent_binding_site	1770	1786	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1124100	.	non_covalent_binding_site	1746	1762	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1124100	.	promoter	1783	1788	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1124100	.	engineered_region	1803	2042	.	+	0	ID=BBa_K1124113;Name=BBa_K1124113
BBa_K1124100	.	mature_transcript_region	1803	1905	.	+	0	ID=BBa_K1124006;Name=BBa_K1124006;Parent=BBa_K1124113
BBa_K1124100	.	sequence_feature	1803	1826	.	+	0	ID=annotation2339033;Name=Target-binding sequence;Parent=BBa_K1124006
BBa_K1124100	.	sequence_feature	1827	1905	.	+	0	ID=annotation2339032;Name=MicC scaffold;Parent=BBa_K1124006
BBa_K1124100	.	terminator	1914	2042	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124113
BBa_K1124100	.	terminator	2002	2042	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	2029	2042	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	2035	2035	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	2009	2028	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	2002	2042	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	1914	1993	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	1925	1968	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	1914	1993	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	2108	2347	.	+	0	ID=BBa_K1124114;Name=BBa_K1124114
BBa_K1124100	.	terminator	2219	2347	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124114
BBa_K1124100	.	terminator	2307	2347	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124100	.	polyA_site	2334	2347	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124100	.	stop_codon	2340	2340	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124100	.	stem_loop	2314	2333	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124100	.	engineered_region	2307	2347	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124100	.	terminator	2219	2298	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124100	.	stem_loop	2230	2273	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124100	.	engineered_region	2219	2298	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124100	.	mature_transcript_region	2108	2210	.	+	0	ID=BBa_K1124007;Name=BBa_K1124007;Parent=BBa_K1124114
BBa_K1124100	.	sequence_feature	2108	2131	.	+	0	ID=annotation2339035;Name=Target-binding sequence;Parent=BBa_K1124007
BBa_K1124100	.	sequence_feature	2132	2210	.	+	0	ID=annotation2339034;Name=MicC scaffold;Parent=BBa_K1124007
>BBa_K1124100
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggactactagatgtc
cagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgt
aaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcct
tagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaa
taacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaa
aaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcac
tcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaac
acctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttc
ttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaa
actacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtactagatga
cagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccagg
agaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgcca
gaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaag
atagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattt
tgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcat
agcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccag
gagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgt
attattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaatta
gctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagc
aggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctg
atagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagatcctcctcgccctt
gctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgg
gcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaac
accgtgcgtgttgactattttacctctggcggtgataatggttgctactagagcgatttttttatcattatagtcattt
tctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggctttttttta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgta
caggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagatcctcctcgccct
tgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccg
ggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagcg
atttttttatcattatagtcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccg
aacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgataga
gatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatg
cgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacg
agggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagtt
catgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttc
aagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagt
tcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctggga
ggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggc
ggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaaca
tcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccac
cggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagagg
agaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaag
tcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgat
aactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacag
gtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaatt
aagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagct
atatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagc
gtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctat
gcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactc
tgatagtgctagtgtagatctctactagagtcacacaggactactagatgtccagattagataaaagtaaagtgattaa
cagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagag
cagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcacc
atactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgcttt
actaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaa
ttagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttag
gttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccatt
attacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgc
ggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacact
gatagtgctagtgtagatcactactagagtcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccag
caggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtg
ttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctt
tttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacggg
ttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttt
tataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtata
ttgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggcc
atactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatac
gaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaa
gaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctg
aatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcct
aggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataat
taataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaa
tattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaat
ggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaat
taattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtctt
atcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagaca
actatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaa
aataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaagga
aaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatga
aactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaa
ttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatacta
gatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaa
tttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgg
gaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggc
agacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagca
gttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttc
ccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggt
gctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaa
acagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaa
aaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacga
cgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatg
agcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaa
atgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatgg
catcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttca
atcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttt
tttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcac
aaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagcca
agctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttg
ggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtaccc
aatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaaaagacgtttggctaa
taattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata