LOCUS BBa_K1124101 5789 bp DNA linear 07-Dec-2025 DEFINITION clock device (plux/tet) (+luxI, +tetR, +aiiA, ++sRNA, +amilGFP) ACCESSION BBa_K1124101 VERSION BBa_K1124101.1 FEATURES Location/Qualifiers misc_feature 1..53 /label=R0062 promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 54..72 /label=tetR misc_feature 53..53 /label=+1 misc_feature 1..20 /label=luxR/HSL promoter 1..72 /label=BBa_K176000 misc_feature 105..183 /label=MicC scaffold misc_feature 81..104 /label=Target-binding sequence misc_feature 81..183 /label=BBa_K1124006 stem_loop 203..246 /label=stem_loop misc_feature 192..271 /label=BBa_B0010 terminator 192..271 /label=BBa_B0010 stem_loop 287..306 /label=T7 TE misc_feature 313..313 /label=stop polyA_site 307..320 /label=polya misc_feature 280..320 /label=BBa_B0012 terminator 280..320 /label=BBa_B0012 terminator 192..320 /label=BBa_B0015 misc_feature 81..320 /label=BBa_K1124113 misc_feature 329..381 /label=R0062 promoter 348..353 /label=-35 promoter 370..375 /label=-10 misc_feature 382..400 /label=tetR misc_feature 381..381 /label=+1 misc_feature 329..348 /label=luxR/HSL promoter 329..400 /label=BBa_K176000 misc_feature 433..511 /label=MicC scaffold misc_feature 409..432 /label=Target-binding sequence misc_feature 409..511 /label=BBa_K1124007 stem_loop 531..574 /label=stem_loop misc_feature 520..599 /label=BBa_B0010 terminator 520..599 /label=BBa_B0010 stem_loop 615..634 /label=T7 TE misc_feature 641..641 /label=stop polyA_site 635..648 /label=polya misc_feature 608..648 /label=BBa_B0012 terminator 608..648 /label=BBa_B0012 terminator 520..648 /label=BBa_B0015 misc_feature 409..648 /label=BBa_K1124114 misc_feature 657..709 /label=R0062 promoter 676..681 /label=-35 promoter 698..703 /label=-10 misc_feature 710..728 /label=tetR misc_feature 709..709 /label=+1 misc_feature 657..676 /label=luxR/HSL promoter 657..728 /label=BBa_K176000 RBS 737..748 /label=B0034 CDS 755..1501 /label=mCherry-LVA misc_feature 755..757 /label=Start misc_feature 1466..1495 /label=ssrA degradation tag: LVA misc_feature 1496..1498 /label=Stop misc_feature 1499..1501 /label=Stop misc_feature 737..1501 /label=BBa_K773003 misc_feature 1514..1517 /label=conserved RBS 1510..1521 /label=BBa_B0034 misc_feature 2107..2138 /label=LVA CDS 1528..2106 /label=luxI misc_feature 1528..2145 /label=BBa_C0061 misc_feature 2146..2170 /label=Help:Barcodes CDS 1528..2145 /label=BBa_C0061 misc_feature 1510..2170 /label=BBa_C0261 RBS 2179..2189 /label=RBS-4\Weaker RBS 2185..2188 /label=RBS misc_feature 2179..2189 /label=BBa_B0033 RBS 2179..2189 /label=BBa_B0033 CDS 2199..2815 /label=tetR misc_feature 2816..2849 /label=SsrA misc_feature 2856..2880 /label=Help:Barcodes CDS 2196..2855 /label=BBa_C0040 misc_feature 2179..2880 /label=BBa_S0107 RBS 2889..2901 /label=RBS-3\Weak RBS 2895..2898 /label=RBS misc_feature 2889..2901 /label=BBa_B0032 RBS 2889..2901 /label=BBa_B0032 misc_feature 2908..2910 /label=start misc_feature 3691..3696 /label=2 misc_feature 3658..3690 /label=LVA CDS 2908..3657 /label=aiiA misc_feature 2908..3696 /label=BBa_C0060 misc_feature 3697..3721 /label=Help:Barcodes CDS 2908..3696 /label=BBa_C0060 stem_loop 3741..3784 /label=stem_loop misc_feature 3730..3809 /label=BBa_B0010 terminator 3730..3809 /label=BBa_B0010 stem_loop 3825..3844 /label=T7 TE misc_feature 3851..3851 /label=stop polyA_site 3845..3858 /label=polya misc_feature 3818..3858 /label=BBa_B0012 terminator 3818..3858 /label=BBa_B0012 terminator 3730..3858 /label=BBa_B0015 misc_feature 2889..3858 /label=BBa_K516022 promoter 3867..3901 /label=BBa_J23101 misc_feature 3914..3917 /label=conserved RBS 3910..3921 /label=BBa_B0034 misc_feature 3928..3929 /label=prefix misc_feature 4101..4101 /label=T misc_feature 4419..4419 /label=A CDS 3928..4677 /label=luxR misc_feature 3928..4683 /label=BBa_C0062 misc_feature 4684..4708 /label=Help:Barcodes CDS 3928..4683 /label=BBa_C0062 stem_loop 4728..4771 /label=stem_loop misc_feature 4717..4796 /label=BBa_B0010 terminator 4717..4796 /label=BBa_B0010 stem_loop 4812..4831 /label=T7 TE misc_feature 4838..4838 /label=stop polyA_site 4832..4845 /label=polya misc_feature 4805..4845 /label=BBa_B0012 terminator 4805..4845 /label=BBa_B0012 terminator 4717..4845 /label=BBa_B0015 promoter 4854..4888 /label=BBa_J23101 misc_feature 4901..4904 /label=conserved RBS 4897..4908 /label=BBa_B0034 misc_feature 5650..5652 /label=stop misc_feature 5647..5649 /label=stop misc_feature 5614..5646 /label=LVA degradation tag RBS 4915..5613 /label=amilGFP misc_feature 4915..5652 /label=BBa_K1124000 stem_loop 5672..5715 /label=stem_loop misc_feature 5661..5740 /label=BBa_B0010 terminator 5661..5740 /label=BBa_B0010 stem_loop 5756..5775 /label=T7 TE misc_feature 5782..5782 /label=stop polyA_site 5776..5789 /label=polya misc_feature 5749..5789 /label=BBa_B0012 terminator 5749..5789 /label=BBa_B0012 terminator 5661..5789 /label=BBa_B0015 misc_feature 4897..5789 /label=BBa_K1124110 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat 61 cagtgataga gatactagag atcctcctcg cccttgctca ccattttctg ttgggccatt 121 gcattgccac tgattttcca acatataaaa agacaagccc gaacagtcgt ccgggctttt 181 ttttactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 241 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 301 tgggcctttc tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa 361 atggtttgtt atagtcgaat atccctatca gtgatagaga tactagagcg atttttttat 421 cattatagtc attttctgtt gggccattgc attgccactg attttccaac atataaaaag 481 acaagcccga acagtcgtcc gggctttttt ttactagagc caggcatcaa ataaaacgaa 541 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 601 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct 661 gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatat ccctatcagt 721 gatagagata ctagagaaag aggagaaata ctagatggtg agcaagggcg aggaggataa 781 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 841 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 901 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 961 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 1021 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 1081 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 1141 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 1201 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 1261 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 1321 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 1381 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 1441 cggcatggac gagctgtaca aggcagcaaa cgacgaaaac tacgctctag tagcttaata 1501 atactagaga aagaggagaa atactagatg actataatga taaaaaaatc ggattttttg 1561 gcaattccat cggaggagta taaaggtatt ctaagtcttc gttatcaagt gtttaagcaa 1621 agacttgagt gggacttagt tgtagaaaat aaccttgaat cagatgagta tgataactca 1681 aatgcagaat atatttatgc ttgtgatgat actgaaaatg taagtggatg ctggcgttta 1741 ttacctacaa caggtgatta tatgctgaaa agtgtttttc ctgaattgct tggtcaacag 1801 agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg taaaaatagc 1861 tcaaagataa ataactctgc tagtgaaatt acaatgaaac tatttgaagc tatatataaa 1921 cacgctgtta gtcaaggtat tacagaatat gtaacagtaa catcaacagc aatagagcga 1981 tttttaaagc gtattaaagt tccttgtcat cgtattggag acaaagaaat tcatgtatta 2041 ggtgatacta aatcggttgt attgtctatg cctattaatg aacagtttaa aaaagcagtc 2101 ttaaatgctg caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag 2161 tgtagatctc tactagagtc acacaggact actagatgtc cagattagat aaaagtaaag 2221 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 2281 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 2341 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 2401 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 2461 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 2521 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 2581 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 2641 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 2701 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 2761 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg 2821 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 2881 tactagagtc acacaggaaa gtactagatg acagtaaaga agctttattt cgtcccagca 2941 ggtcgttgta tgttggatca ttcgtctgtt aatagtacat taacaccagg agaattatta 3001 gacttaccgg tttggtgtta tcttttggag actgaagaag gacctatttt agtagataca 3061 ggtatgccag aaagtgcagt taataatgaa ggtcttttta acggtacatt tgtcgaaggg 3121 caggttttac cgaaaatgac tgaagaagat agaatcgtga atattttaaa acgggttggt 3181 tatgagccgg aagaccttct ttatattatt agttctcact tgcattttga tcatgcagga 3241 ggaaatggcg cttttataaa tacaccaatc attgtacagc gtgctgaata tgaggcggcg 3301 cagcatagcg aagaatattt gaaagaatgt atattgccga atttaaacta caaaatcatt 3361 gaaggtgatt atgaagtcgt accaggagtt caattattgc atacaccagg ccatactcca 3421 gggcatcaat cgctattaat tgagacagaa aaatccggtc ctgtattatt aacgattgat 3481 gcatcgtata cgaaagagaa ttttgaaaat gaagtgccat ttgcgggatt tgattcagaa 3541 ttagctttat cttcaattaa acgtttaaaa gaagtggtga tgaaagagaa gccgattgtt 3601 ttctttggac atgatataga gcaggaaagg ggatgtaaag tgttccctga atatatagct 3661 gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg 3721 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3781 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3841 ggcctttctg cgtttatata ctagagttta cagctagctc agtcctaggt attatgctag 3901 ctactagaga aagaggagaa atactagatg aaaaacataa atgccgacga cacatacaga 3961 ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat 4021 atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg 4081 gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca atattatgat 4141 gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca 4201 attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa 4261 gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat 4321 ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt 4381 ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa 4441 ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta 4501 gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag 4561 cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc 4621 caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa 4681 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 4741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 4801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtttacag 4861 ctagctcagt cctaggtatt atgctagcta ctagagaaag aggagaaata ctagatgtct 4921 tattcaaagc atggcatcgt acaagaaatg aagacgaaat accatatgga aggcagtgtc 4981 aatggccatg aatttacgat cgaaggtgta ggaactgggt acccttacga agggaaacag 5041 atgtccgaat tagtgatcat caagcctgcg ggaaaacccc ttccattctc ctttgacata 5101 ctgtcatcag tctttcaata tggaaaccgt tgcttcacaa agtacccggc agacatgcct 5161 gactatttca agcaagcatt cccagatgga atgtcatatg aaaggtcatt tctatttgag 5221 gatggagcag ttgctacagc cagctggaac attcgtctcg aaggaaattg cttcatccac 5281 aaatccatct ttcatggcgt aaactttccc gctgatggac ccgtaatgaa aaagaagaca 5341 attgactggg ataagtcctt cgaaaaaatg actgtgtcta aagaggtgct aagaggtgac 5401 gtgactatgt ttcttatgct cgaaggaggt ggttctcaca gatgccaatt tcactccact 5461 tacaaaacag agaagccggt cacactgccc ccgaatcatg tcgtagaaca tcaaattgtg 5521 aggaccgacc ttggccaaag tgcaaaaggc tttacagtca agctggaagc acatgccgcg 5581 gctcatgtta accctttgaa ggttaaaagg cctgctgcaa acgacgaaaa ctacgcttta 5641 gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 5701 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 5761 accttcgggt gggcctttct gcgtttata //