##gff-version 3
##sequence-region BBa_K1124102 1 3582
BBa_K1124102	.	engineered_region	2690	3582	.	+	0	ID=BBa_K1124110;Name=BBa_K1124110
BBa_K1124102	.	engineered_region	2708	3445	.	+	0	ID=BBa_K1124000;Name=BBa_K1124000;Parent=BBa_K1124110
BBa_K1124102	.	stop_codon	3440	3442	.	+	0	ID=annotation2338831;Name=stop;Parent=BBa_K1124000
BBa_K1124102	.	stop_codon	3443	3445	.	+	0	ID=annotation2338830;Name=stop;Parent=BBa_K1124000
BBa_K1124102	.	ribosome_entry_site	2708	3406	.	+	0	ID=annotation2338833;Name=amilGFP;Parent=BBa_K1124000
BBa_K1124102	.	tag	3407	3439	.	+	0	ID=annotation2338832;Name=LVA degradation tag;Parent=BBa_K1124000
BBa_K1124102	.	terminator	3454	3582	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124110
BBa_K1124102	.	terminator	3542	3582	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124102	.	polyA_site	3569	3582	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124102	.	stem_loop	3549	3568	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124102	.	stop_codon	3575	3575	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124102	.	engineered_region	3542	3582	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124102	.	terminator	3454	3533	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124102	.	stem_loop	3465	3508	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124102	.	engineered_region	3454	3533	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124102	.	ribosome_entry_site	2690	2701	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124110
BBa_K1124102	.	conserved_region	2694	2697	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124102	.	promoter	1660	1694	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1124102	.	engineered_region	854	1514	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K1124102	.	ribosome_entry_site	854	865	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1124102	.	conserved_region	858	861	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124102	.	CDS	872	1489	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1124102	.	engineered_region	872	1489	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1124102	.	engineered_tag	1490	1514	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1124102	.	CDS	872	1450	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1124102	.	tag	1451	1482	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1124102	.	promoter	2647	2681	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1124102	.	promoter	1	72	.	+	0	ID=BBa_K176000;Name=BBa_K176000
BBa_K1124102	.	promoter	20	25	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124102	.	non_covalent_binding_site	54	72	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124102	.	start_codon	53	53	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124102	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124102	.	promoter	42	47	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124102	.	engineered_region	1	53	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124102	.	terminator	2510	2638	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124102	.	terminator	2598	2638	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124102	.	polyA_site	2625	2638	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124102	.	stem_loop	2605	2624	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124102	.	stop_codon	2631	2631	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124102	.	engineered_region	2598	2638	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124102	.	terminator	2510	2589	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124102	.	stem_loop	2521	2564	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124102	.	engineered_region	2510	2589	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124102	.	engineered_region	81	845	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124102	.	stop_codon	840	842	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124102	.	start_codon	99	101	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124102	.	ribosome_entry_site	81	92	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124102	.	CDS	99	845	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124102	.	stop_codon	843	845	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124102	.	tag	810	839	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124102	.	CDS	1721	2476	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1124102	.	engineered_tag	2477	2501	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1124102	.	engineered_region	1721	2476	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1124102	.	CDS	1721	2470	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1124102	.	point_mutation	1894	1894	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1124102	.	engineered_region	1721	1722	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1124102	.	point_mutation	2212	2212	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1124102	.	terminator	1523	1651	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124102	.	terminator	1611	1651	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124102	.	polyA_site	1638	1651	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124102	.	stem_loop	1618	1637	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124102	.	stop_codon	1644	1644	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124102	.	engineered_region	1611	1651	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124102	.	terminator	1523	1602	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124102	.	stem_loop	1534	1577	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124102	.	engineered_region	1523	1602	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124102	.	ribosome_entry_site	1703	1714	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1124102	.	conserved_region	1707	1710	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1124102
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactaga
gaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaag
gtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccc
agaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacgg
ctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggag
cgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctaca
aggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctc
cgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactac
gacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgg
acatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcat
ggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctacta
gagaaagaggagaaatactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaag
gcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaatt
agtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgt
tgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcat
ttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccat
ctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaa
atgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgcc
aatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggac
cgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggtt
aaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata