LOCUS BBa_K1124103 5120 bp DNA linear 21-Sep-2024 DEFINITION clock device (plux/tet) (-luxI, +tetR, +aiiA, ++sRNA, +amilGFP)(negative feedback only) ACCESSION BBa_K1124103 VERSION BBa_K1124103.1 FEATURES Location/Qualifiers misc_feature 1..53 /label=R0062 promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 54..72 /label=tetR misc_feature 53..53 /label=+1 misc_feature 1..20 /label=luxR/HSL promoter 1..72 /label=BBa_K176000 misc_feature 105..183 /label=MicC scaffold misc_feature 81..104 /label=Target-binding sequence misc_feature 81..183 /label=BBa_K1124006 stem_loop 203..246 /label=stem_loop misc_feature 192..271 /label=BBa_B0010 terminator 192..271 /label=BBa_B0010 stem_loop 287..306 /label=T7 TE misc_feature 313..313 /label=stop polyA_site 307..320 /label=polya misc_feature 280..320 /label=BBa_B0012 terminator 280..320 /label=BBa_B0012 terminator 192..320 /label=BBa_B0015 misc_feature 81..320 /label=BBa_K1124113 misc_feature 329..381 /label=R0062 promoter 348..353 /label=-35 promoter 370..375 /label=-10 misc_feature 382..400 /label=tetR misc_feature 381..381 /label=+1 misc_feature 329..348 /label=luxR/HSL promoter 329..400 /label=BBa_K176000 misc_feature 433..511 /label=MicC scaffold misc_feature 409..432 /label=Target-binding sequence misc_feature 409..511 /label=BBa_K1124007 stem_loop 531..574 /label=stem_loop misc_feature 520..599 /label=BBa_B0010 terminator 520..599 /label=BBa_B0010 stem_loop 615..634 /label=T7 TE misc_feature 641..641 /label=stop polyA_site 635..648 /label=polya misc_feature 608..648 /label=BBa_B0012 terminator 608..648 /label=BBa_B0012 terminator 520..648 /label=BBa_B0015 misc_feature 409..648 /label=BBa_K1124114 misc_feature 657..709 /label=R0062 promoter 676..681 /label=-35 promoter 698..703 /label=-10 misc_feature 710..728 /label=tetR misc_feature 709..709 /label=+1 misc_feature 657..676 /label=luxR/HSL promoter 657..728 /label=BBa_K176000 RBS 737..748 /label=B0034 CDS 755..1501 /label=mCherry-LVA misc_feature 755..757 /label=Start misc_feature 1466..1495 /label=ssrA degradation tag: LVA misc_feature 1496..1498 /label=Stop misc_feature 1499..1501 /label=Stop misc_feature 737..1501 /label=BBa_K773003 RBS 1510..1520 /label=RBS-4\Weaker RBS 1516..1519 /label=RBS misc_feature 1510..1520 /label=BBa_B0033 RBS 1510..1520 /label=BBa_B0033 CDS 1530..2146 /label=tetR misc_feature 2147..2180 /label=SsrA misc_feature 2187..2211 /label=Help:Barcodes CDS 1527..2186 /label=BBa_C0040 misc_feature 1510..2211 /label=BBa_S0107 RBS 2220..2232 /label=RBS-3\Weak RBS 2226..2229 /label=RBS misc_feature 2220..2232 /label=BBa_B0032 RBS 2220..2232 /label=BBa_B0032 misc_feature 2239..2241 /label=start misc_feature 3022..3027 /label=2 misc_feature 2989..3021 /label=LVA CDS 2239..2988 /label=aiiA misc_feature 2239..3027 /label=BBa_C0060 misc_feature 3028..3052 /label=Help:Barcodes CDS 2239..3027 /label=BBa_C0060 stem_loop 3072..3115 /label=stem_loop misc_feature 3061..3140 /label=BBa_B0010 terminator 3061..3140 /label=BBa_B0010 stem_loop 3156..3175 /label=T7 TE misc_feature 3182..3182 /label=stop polyA_site 3176..3189 /label=polya misc_feature 3149..3189 /label=BBa_B0012 terminator 3149..3189 /label=BBa_B0012 terminator 3061..3189 /label=BBa_B0015 misc_feature 2220..3189 /label=BBa_K516022 promoter 3198..3232 /label=BBa_J23101 misc_feature 3245..3248 /label=conserved RBS 3241..3252 /label=BBa_B0034 misc_feature 3259..3260 /label=prefix misc_feature 3432..3432 /label=T misc_feature 3750..3750 /label=A CDS 3259..4008 /label=luxR misc_feature 3259..4014 /label=BBa_C0062 misc_feature 4015..4039 /label=Help:Barcodes CDS 3259..4014 /label=BBa_C0062 stem_loop 4059..4102 /label=stem_loop misc_feature 4048..4127 /label=BBa_B0010 terminator 4048..4127 /label=BBa_B0010 stem_loop 4143..4162 /label=T7 TE misc_feature 4169..4169 /label=stop polyA_site 4163..4176 /label=polya misc_feature 4136..4176 /label=BBa_B0012 terminator 4136..4176 /label=BBa_B0012 terminator 4048..4176 /label=BBa_B0015 promoter 4185..4219 /label=BBa_J23101 misc_feature 4232..4235 /label=conserved RBS 4228..4239 /label=BBa_B0034 misc_feature 4981..4983 /label=stop misc_feature 4978..4980 /label=stop misc_feature 4945..4977 /label=LVA degradation tag RBS 4246..4944 /label=amilGFP misc_feature 4246..4983 /label=BBa_K1124000 stem_loop 5003..5046 /label=stem_loop misc_feature 4992..5071 /label=BBa_B0010 terminator 4992..5071 /label=BBa_B0010 stem_loop 5087..5106 /label=T7 TE misc_feature 5113..5113 /label=stop polyA_site 5107..5120 /label=polya misc_feature 5080..5120 /label=BBa_B0012 terminator 5080..5120 /label=BBa_B0012 terminator 4992..5120 /label=BBa_B0015 misc_feature 4228..5120 /label=BBa_K1124110 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat 61 cagtgataga gatactagag atcctcctcg cccttgctca ccattttctg ttgggccatt 121 gcattgccac tgattttcca acatataaaa agacaagccc gaacagtcgt ccgggctttt 181 ttttactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 241 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 301 tgggcctttc tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa 361 atggtttgtt atagtcgaat atccctatca gtgatagaga tactagagcg atttttttat 421 cattatagtc attttctgtt gggccattgc attgccactg attttccaac atataaaaag 481 acaagcccga acagtcgtcc gggctttttt ttactagagc caggcatcaa ataaaacgaa 541 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 601 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct 661 gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaatat ccctatcagt 721 gatagagata ctagagaaag aggagaaata ctagatggtg agcaagggcg aggaggataa 781 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg 841 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc 901 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca 961 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa 1021 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt 1081 ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca aggtgaagct 1141 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga 1201 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag 1261 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa 1321 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca 1381 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg 1441 cggcatggac gagctgtaca aggcagcaaa cgacgaaaac tacgctctag tagcttaata 1501 atactagagt cacacaggac tactagatgt ccagattaga taaaagtaaa gtgattaaca 1561 gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc 1621 agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc 1681 tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag 1741 gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa 1801 gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa 1861 ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat 1921 atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc 1981 aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac 2041 aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat 2101 tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg 2161 aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca ctactagagt 2221 cacacaggaa agtactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt 2281 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg 2341 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca 2401 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta 2461 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg 2521 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc 2581 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc 2641 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat 2701 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa 2761 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat 2821 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta 2881 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga 2941 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac 3001 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag 3061 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 3121 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 3181 gcgtttatat actagagttt acagctagct cagtcctagg tattatgcta gctactagag 3241 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat 3301 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa 3361 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct 3421 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat 3481 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg 3541 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa 3601 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga 3661 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg 3721 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata 3781 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca 3841 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc 3901 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt 3961 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga 4021 tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag gctcagtcga 4081 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 4141 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtttaca gctagctcag 4201 tcctaggtat tatgctagct actagagaaa gaggagaaat actagatgtc ttattcaaag 4261 catggcatcg tacaagaaat gaagacgaaa taccatatgg aaggcagtgt caatggccat 4321 gaatttacga tcgaaggtgt aggaactggg tacccttacg aagggaaaca gatgtccgaa 4381 ttagtgatca tcaagcctgc gggaaaaccc cttccattct cctttgacat actgtcatca 4441 gtctttcaat atggaaaccg ttgcttcaca aagtacccgg cagacatgcc tgactatttc 4501 aagcaagcat tcccagatgg aatgtcatat gaaaggtcat ttctatttga ggatggagca 4561 gttgctacag ccagctggaa cattcgtctc gaaggaaatt gcttcatcca caaatccatc 4621 tttcatggcg taaactttcc cgctgatgga cccgtaatga aaaagaagac aattgactgg 4681 gataagtcct tcgaaaaaat gactgtgtct aaagaggtgc taagaggtga cgtgactatg 4741 tttcttatgc tcgaaggagg tggttctcac agatgccaat ttcactccac ttacaaaaca 4801 gagaagccgg tcacactgcc cccgaatcat gtcgtagaac atcaaattgt gaggaccgac 4861 cttggccaaa gtgcaaaagg ctttacagtc aagctggaag cacatgccgc ggctcatgtt 4921 aaccctttga aggttaaaag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 4981 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 5041 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 5101 tgggcctttc tgcgtttata //