##gff-version 3 ##sequence-region BBa_K1124103 1 5120 BBa_K1124103 . engineered_region 2220 3189 . + 0 ID=BBa_K516022;Name=BBa_K516022 BBa_K1124103 . terminator 3061 3189 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022 BBa_K1124103 . terminator 3149 3189 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124103 . stem_loop 3156 3175 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124103 . polyA_site 3176 3189 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124103 . stop_codon 3182 3182 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124103 . engineered_region 3149 3189 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124103 . terminator 3061 3140 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124103 . stem_loop 3072 3115 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124103 . engineered_region 3061 3140 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124103 . ribosome_entry_site 2220 2232 . + 0 ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022 BBa_K1124103 . ribosome_entry_site 2220 2232 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K1124103 . ribosome_entry_site 2226 2229 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K1124103 . engineered_region 2220 2232 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K1124103 . CDS 2239 3027 . + 0 ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022 BBa_K1124103 . engineered_tag 3028 3052 . + 0 ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060 BBa_K1124103 . CDS 2239 2988 . + 0 ID=annotation1757;Name=aiiA;Parent=BBa_C0060 BBa_K1124103 . tag 2989 3021 . + 0 ID=annotation1756;Name=LVA;Parent=BBa_C0060 BBa_K1124103 . engineered_region 2239 3027 . + 0 ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060 BBa_K1124103 . start_codon 2239 2241 . + 0 ID=annotation1754;Name=start;Parent=BBa_C0060 BBa_K1124103 . stop_codon 3022 3027 . + 0 ID=annotation1755;Name=2;Parent=BBa_C0060 BBa_K1124103 . promoter 329 400 . + 0 ID=BBa_K176000;Name=BBa_K176000 BBa_K1124103 . promoter 370 375 . + 0 ID=annotation2002609;Name=-10;Parent=BBa_K176000 BBa_K1124103 . non_covalent_binding_site 329 348 . + 0 ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000 BBa_K1124103 . promoter 348 353 . + 0 ID=annotation2002608;Name=-35;Parent=BBa_K176000 BBa_K1124103 . start_codon 381 381 . + 0 ID=annotation2002611;Name=+1;Parent=BBa_K176000 BBa_K1124103 . engineered_region 329 381 . + 0 ID=annotation2002607;Name=R0062;Parent=BBa_K176000 BBa_K1124103 . non_covalent_binding_site 382 400 . + 0 ID=annotation2002610;Name=tetR;Parent=BBa_K176000 BBa_K1124103 . promoter 3198 3232 . + 0 ID=BBa_J23101;Name=BBa_J23101 BBa_K1124103 . ribosome_entry_site 3241 3252 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K1124103 . conserved_region 3245 3248 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1124103 . engineered_region 409 648 . + 0 ID=BBa_K1124114;Name=BBa_K1124114 BBa_K1124103 . mature_transcript_region 409 511 . + 0 ID=BBa_K1124007;Name=BBa_K1124007;Parent=BBa_K1124114 BBa_K1124103 . sequence_feature 409 432 . + 0 ID=annotation2339035;Name=Target-binding sequence;Parent=BBa_K1124007 BBa_K1124103 . sequence_feature 433 511 . + 0 ID=annotation2339034;Name=MicC scaffold;Parent=BBa_K1124007 BBa_K1124103 . terminator 520 648 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124114 BBa_K1124103 . terminator 608 648 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124103 . stem_loop 615 634 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124103 . polyA_site 635 648 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124103 . stop_codon 641 641 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124103 . engineered_region 608 648 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124103 . terminator 520 599 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124103 . stem_loop 531 574 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124103 . engineered_region 520 599 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124103 . CDS 3259 4014 . + 0 ID=BBa_C0062;Name=BBa_C0062 BBa_K1124103 . engineered_tag 4015 4039 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_K1124103 . engineered_region 3259 3260 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_K1124103 . point_mutation 3432 3432 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_K1124103 . engineered_region 3259 4014 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_K1124103 . point_mutation 3750 3750 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_K1124103 . CDS 3259 4008 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_K1124103 . engineered_region 737 1501 . + 0 ID=BBa_K773003;Name=BBa_K773003 BBa_K1124103 . tag 1466 1495 . + 0 ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003 BBa_K1124103 . stop_codon 1499 1501 . + 0 ID=annotation2209513;Name=Stop;Parent=BBa_K773003 BBa_K1124103 . CDS 755 1501 . + 0 ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003 BBa_K1124103 . ribosome_entry_site 737 748 . + 0 ID=annotation2209508;Name=B0034;Parent=BBa_K773003 BBa_K1124103 . start_codon 755 757 . + 0 ID=annotation2209510;Name=Start;Parent=BBa_K773003 BBa_K1124103 . stop_codon 1496 1498 . + 0 ID=annotation2209512;Name=Stop;Parent=BBa_K773003 BBa_K1124103 . engineered_region 81 320 . + 0 ID=BBa_K1124113;Name=BBa_K1124113 BBa_K1124103 . mature_transcript_region 81 183 . + 0 ID=BBa_K1124006;Name=BBa_K1124006;Parent=BBa_K1124113 BBa_K1124103 . sequence_feature 105 183 . + 0 ID=annotation2339032;Name=MicC scaffold;Parent=BBa_K1124006 BBa_K1124103 . sequence_feature 81 104 . + 0 ID=annotation2339033;Name=Target-binding sequence;Parent=BBa_K1124006 BBa_K1124103 . terminator 192 320 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124113 BBa_K1124103 . terminator 280 320 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124103 . stem_loop 287 306 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124103 . polyA_site 307 320 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124103 . stop_codon 313 313 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124103 . engineered_region 280 320 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124103 . terminator 192 271 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124103 . stem_loop 203 246 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124103 . engineered_region 192 271 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124103 . terminator 4048 4176 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1124103 . terminator 4136 4176 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124103 . stem_loop 4143 4162 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124103 . polyA_site 4163 4176 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124103 . stop_codon 4169 4169 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124103 . engineered_region 4136 4176 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124103 . terminator 4048 4127 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124103 . stem_loop 4059 4102 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124103 . engineered_region 4048 4127 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124103 . engineered_region 1510 2211 . + 0 ID=BBa_S0107;Name=BBa_S0107 BBa_K1124103 . CDS 1527 2186 . + 0 ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107 BBa_K1124103 . tag 2147 2180 . + 0 ID=annotation23330;Name=SsrA;Parent=BBa_C0040 BBa_K1124103 . engineered_tag 2187 2211 . + 0 ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040 BBa_K1124103 . CDS 1530 2146 . + 0 ID=annotation23329;Name=tetR;Parent=BBa_C0040 BBa_K1124103 . ribosome_entry_site 1510 1520 . + 0 ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107 BBa_K1124103 . ribosome_entry_site 1510 1520 . + 0 ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033 BBa_K1124103 . ribosome_entry_site 1516 1519 . + 0 ID=annotation1714;Name=RBS;Parent=BBa_B0033 BBa_K1124103 . engineered_region 1510 1520 . + 0 ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033 BBa_K1124103 . promoter 657 728 . + 0 ID=BBa_K176000;Name=BBa_K176000 BBa_K1124103 . promoter 698 703 . + 0 ID=annotation2002609;Name=-10;Parent=BBa_K176000 BBa_K1124103 . non_covalent_binding_site 657 676 . + 0 ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000 BBa_K1124103 . promoter 676 681 . + 0 ID=annotation2002608;Name=-35;Parent=BBa_K176000 BBa_K1124103 . start_codon 709 709 . + 0 ID=annotation2002611;Name=+1;Parent=BBa_K176000 BBa_K1124103 . engineered_region 657 709 . + 0 ID=annotation2002607;Name=R0062;Parent=BBa_K176000 BBa_K1124103 . non_covalent_binding_site 710 728 . + 0 ID=annotation2002610;Name=tetR;Parent=BBa_K176000 BBa_K1124103 . engineered_region 4228 5120 . + 0 ID=BBa_K1124110;Name=BBa_K1124110 BBa_K1124103 . ribosome_entry_site 4228 4239 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124110 BBa_K1124103 . conserved_region 4232 4235 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K1124103 . engineered_region 4246 4983 . + 0 ID=BBa_K1124000;Name=BBa_K1124000;Parent=BBa_K1124110 BBa_K1124103 . stop_codon 4981 4983 . + 0 ID=annotation2338830;Name=stop;Parent=BBa_K1124000 BBa_K1124103 . tag 4945 4977 . + 0 ID=annotation2338832;Name=LVA degradation tag;Parent=BBa_K1124000 BBa_K1124103 . ribosome_entry_site 4246 4944 . + 0 ID=annotation2338833;Name=amilGFP;Parent=BBa_K1124000 BBa_K1124103 . stop_codon 4978 4980 . + 0 ID=annotation2338831;Name=stop;Parent=BBa_K1124000 BBa_K1124103 . terminator 4992 5120 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124110 BBa_K1124103 . terminator 5080 5120 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124103 . stem_loop 5087 5106 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124103 . polyA_site 5107 5120 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124103 . stop_codon 5113 5113 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124103 . engineered_region 5080 5120 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124103 . terminator 4992 5071 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124103 . stem_loop 5003 5046 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124103 . engineered_region 4992 5071 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124103 . promoter 4185 4219 . + 0 ID=BBa_J23101;Name=BBa_J23101 BBa_K1124103 . promoter 1 72 . + 0 ID=BBa_K176000;Name=BBa_K176000 BBa_K1124103 . promoter 42 47 . + 0 ID=annotation2002609;Name=-10;Parent=BBa_K176000 BBa_K1124103 . non_covalent_binding_site 1 20 . + 0 ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000 BBa_K1124103 . promoter 20 25 . + 0 ID=annotation2002608;Name=-35;Parent=BBa_K176000 BBa_K1124103 . start_codon 53 53 . + 0 ID=annotation2002611;Name=+1;Parent=BBa_K176000 BBa_K1124103 . engineered_region 1 53 . + 0 ID=annotation2002607;Name=R0062;Parent=BBa_K176000 BBa_K1124103 . non_covalent_binding_site 54 72 . + 0 ID=annotation2002610;Name=tetR;Parent=BBa_K176000 >BBa_K1124103 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactaga gatcctcctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagc ccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt tatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgat agagatactagagcgatttttttatcattatagtcattttctgttgggccattgcattgccactgattttccaacatat aaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaa agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg cctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatc cctatcagtgatagagatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatc atcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcg agggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacat cctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtcc ttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctcct tgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaa gaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctg aagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcg cctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccga gggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa tactagagtcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaat gaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatg taaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttaga aggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagca aaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaag gtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaaga gcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaatta tttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaat gtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcact actagagtcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatca ttcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaa ggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaag ggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagacct tctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgta cagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaa tcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgct attaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaa gtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagc cgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacga aaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaa aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc accttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactaga gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttt acagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtcttattcaaagcatggc atcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaa ctgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctt tgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaag caagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaaca ttcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaa aaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatg tttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgc ccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctgga agcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacgacgaaaactacgctttagtagct taataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata