BBa_K1124103
type: ComponentDefinition
persistent identity: BBa K1124103
display id: BBa_K1124103
version: 1
was generated by: iGEM to SBOL conversion
description: clock device (plux/tet) (-luxI, +tetR, +aiiA, ++sRNA, +amilGFP)(negative feedback only)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_S0107
component: BBa_K773003
component: BBa_J23101
component: BBa_K516022
component: BBa_C0062
component: BBa_B0034
component: BBa_B0015
component: BBa_K176000
component: BBa_K1124113
component: BBa_K1124114
component: BBa_K1124110
sequence annotation: BBa_K176000
sequence annotation: BBa_K1124113
sequence annotation: BBa_K1124114
sequence annotation: BBa_K773003
sequence annotation: BBa_S0107
sequence annotation: BBa_K516022
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0015
sequence annotation: BBa_K1124110
sequence: https://synbiohub.org/public/igem/BBa_K1124103_sequence/1

BBa_S0107
type: Component
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version: 1
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definition: BBa_S0107
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BBa_K773003
type: Component
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display id: component2342230
version: 1
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BBa_J23101
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BBa_K516022
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definition: BBa_K516022
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BBa_C0062
type: Component
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display id: component2342261
version: 1
top level: BBa_K1124103
definition: luxr
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BBa_B0034
type: Component
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BBa_J23101
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BBa_B0015
type: Component
persistent identity: component2342271
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version: 1
top level: BBa_K1124103
definition: BBa_B0015
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BBa_K176000
type: Component
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definition: pLux/Tet
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BBa_K176000
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BBa_K1124113
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BBa_K176000
type: Component
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definition: pLux/Tet
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BBa_K1124114
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display id: component2342216
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top level: BBa_K1124103
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BBa_K1124110
type: Component
persistent identity: component2342287
display id: component2342287
version: 1
top level: BBa_K1124103
definition: BBa_K1124110
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BBa_K176000
type: SequenceAnnotation
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display id: annotation2342187
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BBa_K1124113
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BBa_K176000
type: SequenceAnnotation
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BBa_K1124114
type: SequenceAnnotation
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display id: annotation2342216
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BBa_K176000
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display id: annotation2342223
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BBa_K773003
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BBa_S0107
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BBa_K516022
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display id: annotation2342255
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BBa_J23101
type: SequenceAnnotation
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display id: annotation2342256
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BBa_B0034
type: SequenceAnnotation
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display id: annotation2342258
version: 1
top level: BBa_K1124103
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component: BBa_B0034

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direction: direction/reverse
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BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2342261
display id: annotation2342261
version: 1
top level: BBa_K1124103
location: https://synbiohub.org/public/igem/BBa_K1124103/annotation2342261/range2342261/1
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2342271
display id: annotation2342271
version: 1
top level: BBa_K1124103
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BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2342272
display id: annotation2342272
version: 1
top level: BBa_K1124103
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BBa_K1124110
type: SequenceAnnotation
persistent identity: annotation2342287
display id: annotation2342287
version: 1
top level: BBa_K1124103
location: https://synbiohub.org/public/igem/BBa_K1124103/annotation2342287/range2342287/1
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display id: range2342287
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BBa_K773003
type: ComponentDefinition
persistent identity: BBa K773003
display id: BBa_K773003
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry-LVA
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We modified the AAV sequence to LVA with PCR.
star: false
creator: Katie Knister
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: mCherry-LVA
sequence annotation: Start
sequence annotation: ssrA degradation tag: LVA
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K773003_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2209508
display id: annotation2209508
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range2209508
display id: range2209508
version: 1
top level: BBa_K773003
direction: direction/forward
start: 1
end: 12
orientation: inline

mCherry-LVA
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persistent identity: annotation2209509
display id: annotation2209509
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2209509
display id: range2209509
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 765
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209510
display id: annotation2209510
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2209510
display id: range2209510
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 21
orientation: inline

ssrA degradation tag: LVA
type: SequenceAnnotation
persistent identity: annotation2209511
display id: annotation2209511
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
role: feature/tag
role: SO:0000324

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display id: range2209511
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direction: direction/forward
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Stop
type: SequenceAnnotation
persistent identity: annotation2209512
display id: annotation2209512
version: 1
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location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
role: feature/stop
role: SO:0000319

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persistent identity: range2209512
display id: range2209512
version: 1
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direction: direction/forward
start: 760
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Stop
type: SequenceAnnotation
persistent identity: annotation2209513
display id: annotation2209513
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2209513
display id: range2209513
version: 1
top level: BBa_K773003
direction: direction/forward
start: 763
end: 765
orientation: inline

BBa_K1124114
type: ComponentDefinition
persistent identity: BBa K1124114
display id: BBa_K1124114
version: 1
was generated by: iGEM to SBOL conversion
description: anti-luxI sRNA (+termintor) (sRNA generator)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming Soon!
owned by: james
owned by: myers
mutable description: Coming Soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Coming Soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K1124007
sequence annotation: BBa_K1124007
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124114_sequence/1

BBa_B0015
type: Component
persistent identity: component2341898
display id: component2341898
version: 1
top level: BBa_K1124114
definition: BBa_B0015
access: public

BBa_K1124007
type: Component
persistent identity: component2341891
display id: component2341891
version: 1
top level: BBa_K1124114
definition: BBa_K1124007
access: public

BBa_K1124007
type: SequenceAnnotation
persistent identity: annotation2341891
display id: annotation2341891
version: 1
top level: BBa_K1124114
location: https://synbiohub.org/public/igem/BBa_K1124114/annotation2341891/range2341891/1
component: BBa_K1124007

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persistent identity: range2341891
display id: range2341891
version: 1
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BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2341898
display id: annotation2341898
version: 1
top level: BBa_K1124114
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persistent identity: range2341898
display id: range2341898
version: 1
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direction: direction/reverse
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end: 240
orientation: inline

BBa_K1124113
type: ComponentDefinition
persistent identity: BBa K1124113
display id: BBa_K1124113
version: 1
was generated by: iGEM to SBOL conversion
description: anti-mCherry sRNA (+termintor) (sRNA generator)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K1124006
sequence annotation: BBa_K1124006
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124113_sequence/1

BBa_B0015
type: Component
persistent identity: component2341908
display id: component2341908
version: 1
top level: BBa_K1124113
definition: BBa_B0015
access: public

BBa_K1124006
type: Component
persistent identity: component2341901
display id: component2341901
version: 1
top level: BBa_K1124113
definition: BBa_K1124006
access: public

BBa_K1124006
type: SequenceAnnotation
persistent identity: annotation2341901
display id: annotation2341901
version: 1
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display id: range2341901
version: 1
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orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2341908
display id: annotation2341908
version: 1
top level: BBa_K1124113
location: https://synbiohub.org/public/igem/BBa_K1124113/annotation2341908/range2341908/1
component: BBa_B0015

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type: Range
persistent identity: range2341908
display id: range2341908
version: 1
top level: BBa_K1124113
direction: direction/reverse
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end: 240
orientation: inline

BBa_S0107
type: ComponentDefinition
persistent identity: BBa S0107
display id: BBa_S0107
version: 1
was generated by: iGEM to SBOL conversion
description: B0033.C0040
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:17Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_B0033
component: BBa_C0040
sequence annotation: BBa_B0033
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_S0107_sequence/1

BBa_B0033
type: Component
persistent identity: component939815
display id: component939815
version: 1
top level: BBa_S0107
definition: BBa_B0033
access: public

BBa_C0040
type: Component
persistent identity: component939826
display id: component939826
version: 1
top level: BBa_S0107
definition: tetR
access: public

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation939815
display id: annotation939815
version: 1
top level: BBa_S0107
location: https://synbiohub.org/public/igem/BBa_S0107/annotation939815/range939815/1
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persistent identity: range939815
display id: range939815
version: 1
top level: BBa_S0107
direction: direction/forward
start: 1
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orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation939826
display id: annotation939826
version: 1
top level: BBa_S0107
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pLux/Tet
type: ComponentDefinition
persistent identity: BBa K176000
display id: BBa_K176000
version: 1
was generated by: iGEM to SBOL conversion
description: pLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPS
created: 2009-05-05T11:00:00Z
modified: 2015-05-08T01:11:00Z
mutable provenance: Tet Operator sequence from BBa_R0040
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by tetR
discontinued: false
dominant: false
experience: experience/Works
group u list: _282_
m user id: 0
owner id: 4010
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: How to arrange the two parts together?
star: false
creator: Danqian Liu, Chao Li, Hao Jiang
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: luxR/HSL
sequence annotation: R0062
sequence annotation: -35
sequence annotation: -10
sequence annotation: +1
sequence annotation: tetR
sequence: https://synbiohub.org/public/igem/BBa_K176000_sequence/1

luxR/HSL
type: SequenceAnnotation
persistent identity: annotation2002612
display id: annotation2002612
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K176000/annotation2002612/range2002612/1
type: Range
persistent identity: range2002612
display id: range2002612
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 20
orientation: inline

R0062
type: SequenceAnnotation
persistent identity: annotation2002607
display id: annotation2002607
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176000/annotation2002607/range2002607/1
type: Range
persistent identity: range2002607
display id: range2002607
version: 1
top level: pLux/Tet
direction: direction/forward
start: 1
end: 53
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2002608
display id: annotation2002608
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002608/range2002608/1
type: Range
persistent identity: range2002608
display id: range2002608
version: 1
top level: pLux/Tet
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002609
display id: annotation2002609
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K176000/annotation2002609/range2002609/1
type: Range
persistent identity: range2002609
display id: range2002609
version: 1
top level: pLux/Tet
direction: direction/forward
start: 42
end: 47
orientation: inline

+1
type: SequenceAnnotation
persistent identity: annotation2002611
display id: annotation2002611
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K176000/annotation2002611/range2002611/1
type: Range
persistent identity: range2002611
display id: range2002611
version: 1
top level: pLux/Tet
direction: direction/forward
start: 53
end: 53
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation2002610
display id: annotation2002610
version: 1
top level: pLux/Tet
location: https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K176000/annotation2002610/range2002610/1
type: Range
persistent identity: range2002610
display id: range2002610
version: 1
top level: pLux/Tet
direction: direction/forward
start: 54
end: 72
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K1124110
type: ComponentDefinition
persistent identity: BBa K1124110
display id: BBa_K1124110
version: 1
was generated by: iGEM to SBOL conversion
description: amilGFP(+LVA) generator(+RBS, +terminator)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K1124000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1124000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124110_sequence/1

BBa_B0034
type: Component
persistent identity: component2341931
display id: component2341931
version: 1
top level: BBa_K1124110
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2341943
display id: component2341943
version: 1
top level: BBa_K1124110
definition: BBa_B0015
access: public

BBa_K1124000
type: Component
persistent identity: component2341936
display id: component2341936
version: 1
top level: BBa_K1124110
definition: amilGFP (+
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2341931
display id: annotation2341931
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341931/range2341931/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341931/range2341931/1
type: Range
persistent identity: range2341931
display id: range2341931
version: 1
top level: BBa_K1124110
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1124000
type: SequenceAnnotation
persistent identity: annotation2341936
display id: annotation2341936
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341936/range2341936/1
component: BBa_K1124000

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341936/range2341936/1
type: Range
persistent identity: range2341936
display id: range2341936
version: 1
top level: BBa_K1124110
direction: direction/reverse
start: 19
end: 756
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2341943
display id: annotation2341943
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341943/range2341943/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341943/range2341943/1
type: Range
persistent identity: range2341943
display id: range2341943
version: 1
top level: BBa_K1124110
direction: direction/reverse
start: 765
end: 893
orientation: inline

BBa_K516022
type: ComponentDefinition
persistent identity: BBa K516022
display id: BBa_K516022
version: 1
was generated by: iGEM to SBOL conversion
description: AiiA protein generator TERM+ RBS+(B0032)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:32Z
mutable provenance: Registry
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AiiA protein generator TERM+ RBS+(B0032)
discontinued: false
dominant: false
experience: experience/Works
group u list: _681_
m user id: 0
owner id: 4150
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: --
star: false
creator: Susanna Zucca, Tommaso Goggia, Paolo Magni
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0015
component: BBa_C0060
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_C0060
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K516022_sequence/1

BBa_B0015
type: Component
persistent identity: component2250570
display id: component2250570
version: 1
top level: BBa_K516022
definition: BBa_B0015
access: public

BBa_C0060
type: Component
persistent identity: component2250560
display id: component2250560
version: 1
top level: BBa_K516022
definition: aiiA
access: public

BBa_B0032
type: Component
persistent identity: component2250556
display id: component2250556
version: 1
top level: BBa_K516022
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2250556
display id: annotation2250556
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250556/range2250556/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K516022/annotation2250556/range2250556/1
type: Range
persistent identity: range2250556
display id: range2250556
version: 1
top level: BBa_K516022
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation2250560
display id: annotation2250560
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250560/range2250560/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_K516022/annotation2250560/range2250560/1
type: Range
persistent identity: range2250560
display id: range2250560
version: 1
top level: BBa_K516022
direction: direction/forward
start: 20
end: 808
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2250570
display id: annotation2250570
version: 1
top level: BBa_K516022
location: https://synbiohub.org/public/igem/BBa_K516022/annotation2250570/range2250570/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K516022/annotation2250570/range2250570/1
type: Range
persistent identity: range2250570
display id: range2250570
version: 1
top level: BBa_K516022
direction: direction/reverse
start: 842
end: 970
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K1124006
type: ComponentDefinition
persistent identity: BBa K1124006
display id: BBa_K1124006
version: 1
was generated by: iGEM to SBOL conversion
description: anti-mCherry sRNA
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: PCR
owned by: james
owned by: myers
mutable description: anti-mCherry sRNA
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13134
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: PCR
star: false
creator: Yuta Otsuka
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: Target-binding sequence
sequence annotation: MicC scaffold
sequence: https://synbiohub.org/public/igem/BBa_K1124006_sequence/1

Target-binding sequence
type: SequenceAnnotation
persistent identity: annotation2339033
display id: annotation2339033
version: 1
top level: BBa_K1124006
location: https://synbiohub.org/public/igem/BBa_K1124006/annotation2339033/range2339033/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1124006/annotation2339033/range2339033/1
type: Range
persistent identity: range2339033
display id: range2339033
version: 1
top level: BBa_K1124006
direction: direction/forward
start: 1
end: 24
orientation: inline

MicC scaffold
type: SequenceAnnotation
persistent identity: annotation2339032
display id: annotation2339032
version: 1
top level: BBa_K1124006
location: https://synbiohub.org/public/igem/BBa_K1124006/annotation2339032/range2339032/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1124006/annotation2339032/range2339032/1
type: Range
persistent identity: range2339032
display id: range2339032
version: 1
top level: BBa_K1124006
direction: direction/forward
start: 25
end: 103
orientation: inline

BBa_K1124007
type: ComponentDefinition
persistent identity: BBa K1124007
display id: BBa_K1124007
version: 1
was generated by: iGEM to SBOL conversion
description: anti-luxI sRNA
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: PCR
owned by: james
owned by: myers
mutable description: anti-luxI sRNA
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13134
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: PCR
star: false
creator: Yuta Otsuka
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: Target-binding sequence
sequence annotation: MicC scaffold
sequence: https://synbiohub.org/public/igem/BBa_K1124007_sequence/1

Target-binding sequence
type: SequenceAnnotation
persistent identity: annotation2339035
display id: annotation2339035
version: 1
top level: BBa_K1124007
location: https://synbiohub.org/public/igem/BBa_K1124007/annotation2339035/range2339035/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1124007/annotation2339035/range2339035/1
type: Range
persistent identity: range2339035
display id: range2339035
version: 1
top level: BBa_K1124007
direction: direction/forward
start: 1
end: 24
orientation: inline

MicC scaffold
type: SequenceAnnotation
persistent identity: annotation2339034
display id: annotation2339034
version: 1
top level: BBa_K1124007
location: https://synbiohub.org/public/igem/BBa_K1124007/annotation2339034/range2339034/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1124007/annotation2339034/range2339034/1
type: Range
persistent identity: range2339034
display id: range2339034
version: 1
top level: BBa_K1124007
direction: direction/forward
start: 25
end: 103
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: start
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
top level: aiiA
direction: direction/forward
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
top level: aiiA
direction: direction/forward
start: 784
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
top level: aiiA
direction: direction/forward
start: 790
end: 814
orientation: inline

amilGFP (+
type: ComponentDefinition
persistent identity: BBa K1124000
display id: BBa_K1124000
version: 1
was generated by: iGEM to SBOL conversion
description: amilGFP (+LVA), yellow reporter protein with degradation tag
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: PCR (template: BBa_K592010, primers shown on our wiki(UT-Tokyo 2013))
owned by: james
owned by: myers
mutable description: amilGFP (+LVA) yellow reporter protein with degradation tag. The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging . It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki. This is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013). amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. Excitation maximum : 503 nm Emission maximum : 512 nm Quantum yield : 0.67 Molar Extinction : 75200 LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus. References Andersen, J. B., Sternberg, C., Poulsen, L. K., Bj??rn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246. Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.
discontinued: false
dominant: false
experience: experience/None
group u list: _1436_
m user id: 0
owner id: 13134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: LVA tag(11 aa) was added by PCR. As in the previous study(1). Additional 2 aa (Stu1 restriction site) was also added, which may not be necessary for unstabilization of the protein. RPAANDENYALVA double stop codon [1]Andersen, J. B., Sternberg, C., Poulsen, L. K., Bj??rn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
star: false
creator: Yuta Otsuka
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: amilGFP
sequence annotation: LVA degradation tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1124000_sequence/1

amilGFP
type: SequenceAnnotation
persistent identity: annotation2338833
display id: annotation2338833
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338833/range2338833/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338833/range2338833/1
type: Range
persistent identity: range2338833
display id: range2338833
version: 1
top level: amilGFP (+
direction: direction/forward
start: 1
end: 699
orientation: inline

LVA degradation tag
type: SequenceAnnotation
persistent identity: annotation2338832
display id: annotation2338832
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338832/range2338832/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338832/range2338832/1
type: Range
persistent identity: range2338832
display id: range2338832
version: 1
top level: amilGFP (+
direction: direction/forward
start: 700
end: 732
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2338831
display id: annotation2338831
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338831/range2338831/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338831/range2338831/1
type: Range
persistent identity: range2338831
display id: range2338831
version: 1
top level: amilGFP (+
direction: direction/forward
start: 733
end: 735
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2338830
display id: annotation2338830
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338830/range2338830/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338830/range2338830/1
type: Range
persistent identity: range2338830
display id: range2338830
version: 1
top level: amilGFP (+
direction: direction/forward
start: 736
end: 738
orientation: inline

https://synbiohub.org/public/igem/BBa_K1124103_sequence/1
type: Sequence
persistent identity: BBa K1124103 sequence
display id: BBa_K1124103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagatcctcctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagcgatttttttatcattatagtcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagtcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K773003_sequence/1
type: Sequence
persistent identity: BBa K773003 sequence
display id: BBa_K773003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124114_sequence/1
type: Sequence
persistent identity: BBa K1124114 sequence
display id: BBa_K1124114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgatttttttatcattatagtcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124113_sequence/1
type: Sequence
persistent identity: BBa K1124113 sequence
display id: BBa_K1124113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctcctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S0107_sequence/1
type: Sequence
persistent identity: BBa S0107 sequence
display id: BBa_S0107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176000_sequence/1
type: Sequence
persistent identity: BBa K176000 sequence
display id: BBa_K176000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124110_sequence/1
type: Sequence
persistent identity: BBa K1124110 sequence
display id: BBa_K1124110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K516022_sequence/1
type: Sequence
persistent identity: BBa K516022 sequence
display id: BBa_K516022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124006_sequence/1
type: Sequence
persistent identity: BBa K1124006 sequence
display id: BBa_K1124006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctcctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124007_sequence/1
type: Sequence
persistent identity: BBa K1124007 sequence
display id: BBa_K1124007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgatttttttatcattatagtcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0060_sequence/1
type: Sequence
persistent identity: BBa C0060 sequence
display id: BBa_C0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124000_sequence/1
type: Sequence
persistent identity: BBa K1124000 sequence
display id: BBa_K1124000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z