LOCUS BBa_K1124104 2913 bp DNA linear 22-Dec-2024 DEFINITION clock device (plux/tet) (-luxI, -tetR, -aiiA, --sRNA, +amilGFP)(AHL sensor) ACCESSION BBa_K1124104 VERSION BBa_K1124104.1 FEATURES Location/Qualifiers misc_feature 1..53 /label=R0062 promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 54..72 /label=tetR misc_feature 53..53 /label=+1 misc_feature 1..20 /label=luxR/HSL promoter 1..72 /label=BBa_K176000 RBS 81..92 /label=B0034 CDS 99..845 /label=mCherry-LVA misc_feature 99..101 /label=Start misc_feature 810..839 /label=ssrA degradation tag: LVA misc_feature 840..842 /label=Stop misc_feature 843..845 /label=Stop misc_feature 81..845 /label=BBa_K773003 stem_loop 865..908 /label=stem_loop misc_feature 854..933 /label=BBa_B0010 terminator 854..933 /label=BBa_B0010 stem_loop 949..968 /label=T7 TE misc_feature 975..975 /label=stop polyA_site 969..982 /label=polya misc_feature 942..982 /label=BBa_B0012 terminator 942..982 /label=BBa_B0012 terminator 854..982 /label=BBa_B0015 promoter 991..1025 /label=BBa_J23101 misc_feature 1038..1041 /label=conserved RBS 1034..1045 /label=BBa_B0034 misc_feature 1052..1053 /label=prefix misc_feature 1225..1225 /label=T misc_feature 1543..1543 /label=A CDS 1052..1801 /label=luxR misc_feature 1052..1807 /label=BBa_C0062 misc_feature 1808..1832 /label=Help:Barcodes CDS 1052..1807 /label=BBa_C0062 stem_loop 1852..1895 /label=stem_loop misc_feature 1841..1920 /label=BBa_B0010 terminator 1841..1920 /label=BBa_B0010 stem_loop 1936..1955 /label=T7 TE misc_feature 1962..1962 /label=stop polyA_site 1956..1969 /label=polya misc_feature 1929..1969 /label=BBa_B0012 terminator 1929..1969 /label=BBa_B0012 terminator 1841..1969 /label=BBa_B0015 promoter 1978..2012 /label=BBa_J23101 misc_feature 2025..2028 /label=conserved RBS 2021..2032 /label=BBa_B0034 misc_feature 2774..2776 /label=stop misc_feature 2771..2773 /label=stop misc_feature 2738..2770 /label=LVA degradation tag RBS 2039..2737 /label=amilGFP misc_feature 2039..2776 /label=BBa_K1124000 stem_loop 2796..2839 /label=stem_loop misc_feature 2785..2864 /label=BBa_B0010 terminator 2785..2864 /label=BBa_B0010 stem_loop 2880..2899 /label=T7 TE misc_feature 2906..2906 /label=stop polyA_site 2900..2913 /label=polya misc_feature 2873..2913 /label=BBa_B0012 terminator 2873..2913 /label=BBa_B0012 terminator 2785..2913 /label=BBa_B0015 misc_feature 2021..2913 /label=BBa_K1124110 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat 61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg 121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga 481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt 841 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 961 ggtgggcctt tctgcgttta tatactagag tttacagcta gctcagtcct aggtattatg 1021 ctagctacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata 1081 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc 1141 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc 1201 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta 1261 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc 1321 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat 1381 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa 1441 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt 1501 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg 1561 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg 1621 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag 1681 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg 1741 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa 1801 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga 1861 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1921 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttt 1981 acagctagct cagtcctagg tattatgcta gctactagag aaagaggaga aatactagat 2041 gtcttattca aagcatggca tcgtacaaga aatgaagacg aaataccata tggaaggcag 2101 tgtcaatggc catgaattta cgatcgaagg tgtaggaact gggtaccctt acgaagggaa 2161 acagatgtcc gaattagtga tcatcaagcc tgcgggaaaa ccccttccat tctcctttga 2221 catactgtca tcagtctttc aatatggaaa ccgttgcttc acaaagtacc cggcagacat 2281 gcctgactat ttcaagcaag cattcccaga tggaatgtca tatgaaaggt catttctatt 2341 tgaggatgga gcagttgcta cagccagctg gaacattcgt ctcgaaggaa attgcttcat 2401 ccacaaatcc atctttcatg gcgtaaactt tcccgctgat ggacccgtaa tgaaaaagaa 2461 gacaattgac tgggataagt ccttcgaaaa aatgactgtg tctaaagagg tgctaagagg 2521 tgacgtgact atgtttctta tgctcgaagg aggtggttct cacagatgcc aatttcactc 2581 cacttacaaa acagagaagc cggtcacact gcccccgaat catgtcgtag aacatcaaat 2641 tgtgaggacc gaccttggcc aaagtgcaaa aggctttaca gtcaagctgg aagcacatgc 2701 cgcggctcat gttaaccctt tgaaggttaa aaggcctgct gcaaacgacg aaaactacgc 2761 tttagtagct taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 2821 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 2881 gctcaccttc gggtgggcct ttctgcgttt ata //