##gff-version 3
##sequence-region BBa_K1124104 1 2913
BBa_K1124104	.	promoter	1978	2012	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1124104	.	promoter	991	1025	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1124104	.	CDS	1052	1807	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1124104	.	engineered_region	1052	1053	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1124104	.	engineered_region	1052	1807	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1124104	.	engineered_tag	1808	1832	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1124104	.	point_mutation	1543	1543	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1124104	.	point_mutation	1225	1225	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1124104	.	CDS	1052	1801	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1124104	.	ribosome_entry_site	1034	1045	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1124104	.	conserved_region	1038	1041	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124104	.	terminator	1841	1969	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124104	.	terminator	1929	1969	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124104	.	engineered_region	1929	1969	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124104	.	stem_loop	1936	1955	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124104	.	polyA_site	1956	1969	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124104	.	stop_codon	1962	1962	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124104	.	terminator	1841	1920	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124104	.	engineered_region	1841	1920	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124104	.	stem_loop	1852	1895	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124104	.	terminator	854	982	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124104	.	terminator	942	982	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124104	.	engineered_region	942	982	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124104	.	stem_loop	949	968	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124104	.	polyA_site	969	982	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124104	.	stop_codon	975	975	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124104	.	terminator	854	933	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124104	.	engineered_region	854	933	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124104	.	stem_loop	865	908	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124104	.	engineered_region	2021	2913	.	+	0	ID=BBa_K1124110;Name=BBa_K1124110
BBa_K1124104	.	engineered_region	2039	2776	.	+	0	ID=BBa_K1124000;Name=BBa_K1124000;Parent=BBa_K1124110
BBa_K1124104	.	stop_codon	2774	2776	.	+	0	ID=annotation2338830;Name=stop;Parent=BBa_K1124000
BBa_K1124104	.	stop_codon	2771	2773	.	+	0	ID=annotation2338831;Name=stop;Parent=BBa_K1124000
BBa_K1124104	.	ribosome_entry_site	2039	2737	.	+	0	ID=annotation2338833;Name=amilGFP;Parent=BBa_K1124000
BBa_K1124104	.	tag	2738	2770	.	+	0	ID=annotation2338832;Name=LVA degradation tag;Parent=BBa_K1124000
BBa_K1124104	.	terminator	2785	2913	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124110
BBa_K1124104	.	terminator	2873	2913	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124104	.	engineered_region	2873	2913	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124104	.	stem_loop	2880	2899	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124104	.	polyA_site	2900	2913	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124104	.	stop_codon	2906	2906	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124104	.	terminator	2785	2864	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124104	.	engineered_region	2785	2864	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124104	.	stem_loop	2796	2839	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124104	.	ribosome_entry_site	2021	2032	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124110
BBa_K1124104	.	conserved_region	2025	2028	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124104	.	promoter	1	72	.	+	0	ID=BBa_K176000;Name=BBa_K176000
BBa_K1124104	.	promoter	20	25	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124104	.	non_covalent_binding_site	54	72	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124104	.	start_codon	53	53	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124104	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124104	.	promoter	42	47	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124104	.	engineered_region	1	53	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124104	.	engineered_region	81	845	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124104	.	stop_codon	843	845	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124104	.	CDS	99	845	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124104	.	tag	810	839	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124104	.	stop_codon	840	842	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124104	.	start_codon	99	101	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124104	.	ribosome_entry_site	81	92	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
>BBa_K1124104
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactaga
gaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaag
gtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccc
agaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacgg
ctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggag
cgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctaca
aggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctc
cgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactac
gacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgg
acatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcat
ggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagcta
ctagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagctt
gtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgat
catttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgat
gacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttg
aaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagttt
ccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagttta
tttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaata
ataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatat
ttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaac
cgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagt
gctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactag
agtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtcttattcaaagc
atggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgt
aggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattc
tcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatt
tcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctg
gaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgta
atgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtga
ctatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcac
actgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaag
ctggaagcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacgacgaaaactacgctttag
tagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata