BBa_K1124009
1
BBa_K1124009
anti-tyrR sRNA
2013-09-10T11:00:00Z
2015-05-08T01:09:17Z
PCR
anti-tyrR sRNA
false
false
_1436_
0
13134
9
Not in stock
false
PCR
false
Yuta Otsuka
annotation2339040
1
Target-binding sequence
range2339040
1
1
24
annotation2339038
1
MicC scaffold
range2339038
1
25
103
BBa_K1124117
1
BBa_K1124117
anti-csrA sRNA (+termintor) (sRNA generator)
2013-09-14T11:00:00Z
2015-05-08T01:09:17Z
Coming soon!
Coming soon!
false
false
_1436_
0
13160
9
It's complicated
false
Coming soon!
false
Takahiro Yamada
component2341861
1
BBa_K1124010
component2341868
1
BBa_B0015
annotation2341861
1
BBa_K1124010
range2341861
1
1
103
annotation2341868
1
BBa_B0015
range2341868
1
112
240
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K1124106
1
BBa_K1124106
plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (tyrosine synthesis device)
2013-09-14T11:00:00Z
2015-05-08T01:09:17Z
Coming soon!
Coming soon!
false
false
_1436_
0
13160
9
Not in stock
false
Coming soon!
false
Takahiro Yamada
component2352065
1
BBa_K1124117
component2352049
1
BBa_K1124116
component2352035
1
BBa_R0051
component2352051
1
BBa_R0051
annotation2352051
1
BBa_R0051
range2352051
1
306
354
annotation2352065
1
BBa_K1124117
range2352065
1
363
602
annotation2352035
1
BBa_R0051
range2352035
1
1
49
annotation2352049
1
BBa_K1124116
range2352049
1
58
297
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_R0051
1
cI lam
promoter (lambda cI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
<a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
Released HQ 2013
The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor.
true
Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
annotation7067
1
BBa_R0051
range7067
1
1
49
annotation2022
1
-10
range2022
1
38
43
annotation2024
1
OR1
range2024
1
25
41
annotation2023
1
-35
range2023
1
15
20
annotation2025
1
OR2
range2025
1
1
17
BBa_K1124116
1
BBa_K1124116
anti-tyrR sRNA (+termintor) (sRNA generator)
2013-09-14T11:00:00Z
2015-05-08T01:09:17Z
Coming soon!
Coming soon!
false
false
_1436_
0
13160
9
It's complicated
false
Coming soon!
false
Takahiro Yamada
component2341871
1
BBa_K1124009
component2341878
1
BBa_B0015
annotation2341871
1
BBa_K1124009
range2341871
1
1
103
annotation2341878
1
BBa_B0015
range2341878
1
112
240
BBa_K1124010
1
BBa_K1124010
anti-csrA sRNA
2013-09-10T11:00:00Z
2015-05-08T01:09:17Z
PCR
anti-csrA sRNA
false
false
_1436_
0
13134
9
Not in stock
false
PCR
false
Yuta Otsuka
annotation2339041
1
Target-binding sequence
range2339041
1
1
24
annotation2339039
1
MicC scaffold
range2339039
1
25
103
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1124106_sequence
1
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagttcacaaaagacttccagacgcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaactcgacgagtcagaatcagcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1124010_sequence
1
aactcgacgagtcagaatcagcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttt
BBa_K1124009_sequence
1
ttcacaaaagacttccagacgcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttt
BBa_K1124117_sequence
1
aactcgacgagtcagaatcagcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0051_sequence
1
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
BBa_K1124116_sequence
1
ttcacaaaagacttccagacgcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z