LOCUS BBa_K1124107 2917 bp DNA linear 27-Dec-2024 DEFINITION pLac-hpaBC-plambda-sRNA(anti-tyrR)-plambda-sRNA (anti-csrA) (L-DOPA synthesis device) ACCESSION BBa_K1124107 VERSION BBa_K1124107.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..77 /label=(RBS (natural)) CDS 78..1637 /label=hpaB misc_feature 149..149 /label=PstI site deletion (silent mutation G to A) misc_feature 1487..1487 /label=PstI site deletion (silent mutation G to T) misc_feature 1638..1640 /label=stop RBS 1641..1657 /label=(RBS (natural)) CDS 1658..2167 /label=hpaC misc_feature 2168..2170 /label=stop misc_feature 64..2170 /label=BBa_K1124011 stem_loop 2190..2233 /label=stem_loop misc_feature 2179..2258 /label=BBa_B0010 terminator 2179..2258 /label=BBa_B0010 stem_loop 2274..2293 /label=T7 TE misc_feature 2300..2300 /label=stop polyA_site 2294..2307 /label=polya misc_feature 2267..2307 /label=BBa_B0012 terminator 2267..2307 /label=BBa_B0012 terminator 2179..2307 /label=BBa_B0015 stem_loop 2495..2538 /label=stem_loop misc_feature 2484..2563 /label=BBa_B0010 terminator 2484..2563 /label=BBa_B0010 stem_loop 2579..2598 /label=T7 TE misc_feature 2605..2605 /label=stop polyA_site 2599..2612 /label=polya misc_feature 2572..2612 /label=BBa_B0012 terminator 2572..2612 /label=BBa_B0012 terminator 2484..2612 /label=BBa_B0015 stem_loop 2800..2843 /label=stem_loop misc_feature 2789..2868 /label=BBa_B0010 terminator 2789..2868 /label=BBa_B0010 stem_loop 2884..2903 /label=T7 TE misc_feature 2910..2910 /label=stop polyA_site 2904..2917 /label=polya misc_feature 2877..2917 /label=BBa_B0012 terminator 2877..2917 /label=BBa_B0012 terminator 2789..2917 /label=BBa_B0015 promoter 2353..2358 /label=-10 promoter 2330..2335 /label=-35 misc_feature 2340..2356 /label=OR1 misc_feature 2316..2332 /label=OR2 misc_feature 2316..2364 /label=BBa_R0051 promoter 2316..2364 /label=BBa_R0051 misc_feature 2397..2475 /label=MicC scaffold misc_feature 2373..2396 /label=Target-binding sequence misc_feature 2373..2475 /label=BBa_K1124009 stem_loop 2495..2538 /label=stem_loop misc_feature 2484..2563 /label=BBa_B0010 terminator 2484..2563 /label=BBa_B0010 stem_loop 2579..2598 /label=T7 TE misc_feature 2605..2605 /label=stop polyA_site 2599..2612 /label=polya misc_feature 2572..2612 /label=BBa_B0012 terminator 2572..2612 /label=BBa_B0012 terminator 2484..2612 /label=BBa_B0015 misc_feature 2373..2612 /label=BBa_K1124116 promoter 2658..2663 /label=-10 promoter 2635..2640 /label=-35 misc_feature 2645..2661 /label=OR1 misc_feature 2621..2637 /label=OR2 misc_feature 2621..2669 /label=BBa_R0051 promoter 2621..2669 /label=BBa_R0051 misc_feature 2702..2780 /label=MicC scaffold misc_feature 2678..2701 /label=Target-binding sequence misc_feature 2678..2780 /label=BBa_K1124010 stem_loop 2800..2843 /label=stem_loop misc_feature 2789..2868 /label=BBa_B0010 terminator 2789..2868 /label=BBa_B0010 stem_loop 2884..2903 /label=T7 TE misc_feature 2910..2910 /label=stop polyA_site 2904..2917 /label=polya misc_feature 2877..2917 /label=BBa_B0012 terminator 2877..2917 /label=BBa_B0012 terminator 2789..2917 /label=BBa_B0015 misc_feature 2678..2917 /label=BBa_K1124117 misc_feature 2316..2917 /label=BBa_K1124106 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagctgtaga ggtcgacatg aaaccagaag atttccgcgc cagtacccaa cgtcctttca 121 ccggggaaga gtatctgaaa agcctgcaag atggtcgcga gatctatatc tatggcgagc 181 gagtgaaaga cgtcaccact catccggcat ttcgtaatgc ggcagcgtct gttgcccagc 241 tgtacgacgc actgcacaaa ccggagatgc aggactctct gtgttggaac accgacaccg 301 gcagcggcgg ctatacccat aaattcttcc gcgtggcgaa aagtgccgac gacctgcgcc 361 agcaacgcga cgccatcgct gagtggtcac gcctgagcta tggctggatg ggccgtaccc 421 cagactacaa agccgctttc ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt 481 tcgagcagaa cgcccgtaac tggtacaccc gtattcagga aactggcctc tactttaacc 541 acgcgattgt taacccaccg atcgatcgtc atttgccgac cgataaagtg aaagacgttt 601 acatcaagct ggaaaaagag actgacgccg ggattatcgt cagcggtgcg aaagtggttg 661 ccaccaactc ggcgctgact cactacaaca tgattggctt cggctcggca caagtgatgg 721 gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat ggatgccgat ggcgtgaaat 781 taatctcccg cgcctcttat gagatggtcg cgggtgctac cggctcgcca tacgactacc 841 cgctctccag ccgcttcgat gagaacgatg cgattctggt gatggataac gtgctgattc 901 catgggaaaa cgtgctgatc taccgcgatt ttgatcgctg ccgtcgctgg acgatggaag 961 gcggttttgc ccgtatgtat ccgctgcaag cctgtgtgcg cctggcagtg aaattagact 1021 tcattacggc actgctgaaa aaatcactcg aatgtaccgg caccctggag ttccgtggtg 1081 tgcaggccga tctcggtgaa gtggtagcgt ggcgcaacac cttctgggca ttgagtgact 1141 cgatgtgttc agaagcaacg ccgtgggtca acggggctta tttaccggat catgccgcac 1201 tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa gatcaaaaac attatcgaac 1261 gcaacgttac cagtggcctg atctatctcc cttccagtgc ccgtgacctg aataatccgc 1321 agatcgacca gtatctggcg aagtatgtgc gcggttcgaa cggtatggat cacgtccagc 1381 gcatcaagat cctcaaactg atgtgggatg ctattggcag cgaatttggt ggtcgtcacg 1441 aactgtatga aatcaactac tccggtagcc aggatgagat tcgccttcag tgtctgcgcc 1501 aggcacaaaa ctccggcaat atggacaaga tgatggcgat ggttgatcgc tgcctgtcgg 1561 aatacgacca ggacggctgg actgtgccgc acctgcacaa caacgacgat atcaacatgc 1621 tggataagct gctgaaataa cgcagcagga ggttaagatg caattagatg aacaacgcct 1681 gcgctttcgt gacgcgatgg ccagcctgtc ggcagcggta aatattatca ccaccgaggg 1741 cgacgccgga caatgcggga ttacggcaac ggccgtctgc tcggtcacgg atacaccacc 1801 gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac ccggtttttc agggcaacgg 1861 caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg atggcacgcc acttcgcggg 1921 catgacaggc atggcgatgg aagagcgttt tagcctctca tgctggcaaa aaggtccgct 1981 ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa ggtgagatcc gcgatgtgca 2041 ggcaattggc acacatctgg tgtatctggt ggagattaaa aacatcatcc tcagtgcaga 2101 aggtcatgga cttatctact ttaaacgccg tttccatccg gtgatgctgg aaatggaagc 2161 tgcgatttaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 2221 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 2281 cttcgggtgg gcctttctgc gtttatatac tagagtaaca ccgtgcgtgt tgactatttt 2341 acctctggcg gtgataatgg ttgctactag agttcacaaa agacttccag acgcattttc 2401 tgttgggcca ttgcattgcc actgattttc caacatataa aaagacaagc ccgaacagtc 2461 gtccgggctt ttttttacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 2521 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 2581 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact 2641 attttacctc tggcggtgat aatggttgct actagagaac tcgacgagtc agaatcagca 2701 ttttctgttg ggccattgca ttgccactga ttttccaaca tataaaaaga caagcccgaa 2761 cagtcgtccg ggcttttttt tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2821 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2881 actggctcac cttcgggtgg gcctttctgc gtttata //