##gff-version 3 ##sequence-region BBa_K1124107 1 2917 BBa_K1124107 . engineered_region 2316 2917 . + 0 ID=BBa_K1124106;Name=BBa_K1124106 BBa_K1124107 . promoter 2316 2364 . + 0 ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K1124106 BBa_K1124107 . promoter 2353 2358 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1124107 . promoter 2330 2335 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1124107 . engineered_region 2316 2364 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1124107 . non_covalent_binding_site 2340 2356 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1124107 . non_covalent_binding_site 2316 2332 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1124107 . engineered_region 2678 2917 . + 0 ID=BBa_K1124117;Name=BBa_K1124117;Parent=BBa_K1124106 BBa_K1124107 . terminator 2789 2917 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124117 BBa_K1124107 . terminator 2789 2868 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2789 2868 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124107 . stem_loop 2800 2843 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124107 . terminator 2877 2917 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2877 2917 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124107 . stop_codon 2910 2910 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124107 . stem_loop 2884 2903 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124107 . polyA_site 2904 2917 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124107 . mature_transcript_region 2678 2780 . + 0 ID=BBa_K1124010;Name=BBa_K1124010;Parent=BBa_K1124117 BBa_K1124107 . sequence_feature 2678 2701 . + 0 ID=annotation2339041;Name=Target-binding sequence;Parent=BBa_K1124010 BBa_K1124107 . sequence_feature 2702 2780 . + 0 ID=annotation2339039;Name=MicC scaffold;Parent=BBa_K1124010 BBa_K1124107 . promoter 2621 2669 . + 0 ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K1124106 BBa_K1124107 . promoter 2658 2663 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K1124107 . promoter 2635 2640 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K1124107 . engineered_region 2621 2669 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K1124107 . non_covalent_binding_site 2645 2661 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K1124107 . non_covalent_binding_site 2621 2637 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K1124107 . engineered_region 2373 2612 . + 0 ID=BBa_K1124116;Name=BBa_K1124116;Parent=BBa_K1124106 BBa_K1124107 . terminator 2484 2612 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124116 BBa_K1124107 . terminator 2484 2563 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2484 2563 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124107 . stem_loop 2495 2538 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124107 . terminator 2572 2612 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2572 2612 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124107 . stop_codon 2605 2605 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124107 . stem_loop 2579 2598 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124107 . polyA_site 2599 2612 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124107 . mature_transcript_region 2373 2475 . + 0 ID=BBa_K1124009;Name=BBa_K1124009;Parent=BBa_K1124116 BBa_K1124107 . sequence_feature 2373 2396 . + 0 ID=annotation2339040;Name=Target-binding sequence;Parent=BBa_K1124009 BBa_K1124107 . sequence_feature 2397 2475 . + 0 ID=annotation2339038;Name=MicC scaffold;Parent=BBa_K1124009 BBa_K1124107 . terminator 2179 2307 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1124107 . terminator 2179 2258 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2179 2258 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124107 . stem_loop 2190 2233 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124107 . terminator 2267 2307 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2267 2307 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124107 . stop_codon 2300 2300 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124107 . stem_loop 2274 2293 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124107 . polyA_site 2294 2307 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124107 . terminator 2484 2612 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1124107 . terminator 2484 2563 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2484 2563 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124107 . stem_loop 2495 2538 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124107 . terminator 2572 2612 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2572 2612 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124107 . stop_codon 2605 2605 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124107 . stem_loop 2579 2598 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124107 . polyA_site 2599 2612 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124107 . promoter 1 54 . + 0 ID=BBa_R0011;Name=BBa_R0011 BBa_K1124107 . promoter 43 48 . + 0 ID=annotation2002;Name=-10;Parent=BBa_R0011 BBa_K1124107 . promoter 20 25 . + 0 ID=annotation2000;Name=-35;Parent=BBa_R0011 BBa_K1124107 . operator 26 42 . + 0 ID=annotation2001;Name=lac O1;Parent=BBa_R0011 BBa_K1124107 . engineered_region 1 54 . + 0 ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011 BBa_K1124107 . operator 3 19 . + 0 ID=annotation1999;Name=lac O1;Parent=BBa_R0011 BBa_K1124107 . terminator 2789 2917 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K1124107 . terminator 2789 2868 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2789 2868 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K1124107 . stem_loop 2800 2843 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K1124107 . terminator 2877 2917 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K1124107 . engineered_region 2877 2917 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K1124107 . stop_codon 2910 2910 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K1124107 . stem_loop 2884 2903 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K1124107 . polyA_site 2904 2917 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K1124107 . engineered_region 64 2170 . + 0 ID=BBa_K1124011;Name=BBa_K1124011 BBa_K1124107 . point_mutation 149 149 . + 0 ID=annotation2341756;Name=PstI site deletion (silent mutation G to A);Parent=BBa_K1124011 BBa_K1124107 . ribosome_entry_site 64 77 . + 0 ID=annotation2341754;Name=(RBS (natural));Parent=BBa_K1124011 BBa_K1124107 . stop_codon 1638 1640 . + 0 ID=annotation2341758;Name=stop;Parent=BBa_K1124011 BBa_K1124107 . point_mutation 1487 1487 . + 0 ID=annotation2341757;Name=PstI site deletion (silent mutation G to T);Parent=BBa_K1124011 BBa_K1124107 . stop_codon 2168 2170 . + 0 ID=annotation2341761;Name=stop;Parent=BBa_K1124011 BBa_K1124107 . CDS 78 1637 . + 0 ID=annotation2341755;Name=hpaB;Parent=BBa_K1124011 BBa_K1124107 . ribosome_entry_site 1641 1657 . + 0 ID=annotation2341759;Name=(RBS (natural));Parent=BBa_K1124011 BBa_K1124107 . CDS 1658 2167 . + 0 ID=annotation2341760;Name=hpaC;Parent=BBa_K1124011 >BBa_K1124107 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagctgtagaggtcgacat gaaaccagaagatttccgcgccagtacccaacgtcctttcaccggggaagagtatctgaaaagcctgcaagatggtcgc gagatctatatctatggcgagcgagtgaaagacgtcaccactcatccggcatttcgtaatgcggcagcgtctgttgccc agctgtacgacgcactgcacaaaccggagatgcaggactctctgtgttggaacaccgacaccggcagcggcggctatac ccataaattcttccgcgtggcgaaaagtgccgacgacctgcgccagcaacgcgacgccatcgctgagtggtcacgcctg agctatggctggatgggccgtaccccagactacaaagccgctttcggttgcgcactgggcgcgaatccgggcttttacg gtcagttcgagcagaacgcccgtaactggtacacccgtattcaggaaactggcctctactttaaccacgcgattgttaa cccaccgatcgatcgtcatttgccgaccgataaagtgaaagacgtttacatcaagctggaaaaagagactgacgccggg attatcgtcagcggtgcgaaagtggttgccaccaactcggcgctgactcactacaacatgattggcttcggctcggcac aagtgatgggcgaaaacccggacttcgcactgatgttcgttgcgccaatggatgccgatggcgtgaaattaatctcccg cgcctcttatgagatggtcgcgggtgctaccggctcgccatacgactacccgctctccagccgcttcgatgagaacgat gcgattctggtgatggataacgtgctgattccatgggaaaacgtgctgatctaccgcgattttgatcgctgccgtcgct ggacgatggaaggcggttttgcccgtatgtatccgctgcaagcctgtgtgcgcctggcagtgaaattagacttcattac ggcactgctgaaaaaatcactcgaatgtaccggcaccctggagttccgtggtgtgcaggccgatctcggtgaagtggta gcgtggcgcaacaccttctgggcattgagtgactcgatgtgttcagaagcaacgccgtgggtcaacggggcttatttac cggatcatgccgcactgcaaacctatcgcgtactggcaccaatggcctacgcgaagatcaaaaacattatcgaacgcaa cgttaccagtggcctgatctatctcccttccagtgcccgtgacctgaataatccgcagatcgaccagtatctggcgaag tatgtgcgcggttcgaacggtatggatcacgtccagcgcatcaagatcctcaaactgatgtgggatgctattggcagcg aatttggtggtcgtcacgaactgtatgaaatcaactactccggtagccaggatgagattcgccttcagtgtctgcgcca ggcacaaaactccggcaatatggacaagatgatggcgatggttgatcgctgcctgtcggaatacgaccaggacggctgg actgtgccgcacctgcacaacaacgacgatatcaacatgctggataagctgctgaaataacgcagcaggaggttaagat gcaattagatgaacaacgcctgcgctttcgtgacgcgatggccagcctgtcggcagcggtaaatattatcaccaccgag ggcgacgccggacaatgcgggattacggcaacggccgtctgctcggtcacggatacaccaccgtcgctgatggtgtgca ttaacgccaacagtgcgatgaacccggtttttcagggcaacggcaagttgtgcgtcaacgtcctcaaccatgagcagga actgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctctcatgctggcaaaaaggt ccgctggcgcagccggtgctaaaaggttcgctggccagtcttgaaggtgagatccgcgatgtgcaggcaattggcacac atctggtgtatctggtggagattaaaaacatcatcctcagtgcagaaggtcatggacttatctactttaaacgccgttt ccatccggtgatgctggaaatggaagctgcgatttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaa agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg cctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactag agttcacaaaagacttccagacgcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaag cccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt ttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaactcgacg agtcagaatcagcattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtc gtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata