##gff-version 3
##sequence-region BBa_K1124108 1 1883
BBa_K1124108	.	promoter	968	1002	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1124108	.	engineered_region	58	822	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124108	.	ribosome_entry_site	58	69	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124108	.	start_codon	76	78	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124108	.	stop_codon	820	822	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124108	.	tag	787	816	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124108	.	stop_codon	817	819	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124108	.	CDS	76	822	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124108	.	terminator	831	959	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124108	.	terminator	919	959	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124108	.	stem_loop	926	945	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124108	.	engineered_region	919	959	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124108	.	stop_codon	952	952	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124108	.	polyA_site	946	959	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124108	.	terminator	831	910	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124108	.	engineered_region	831	910	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124108	.	stem_loop	842	885	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124108	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1124108	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1124108	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1124108	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1124108	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1124108	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1124108	.	engineered_region	1011	1883	.	+	0	ID=BBa_K1124118;Name=BBa_K1124118
BBa_K1124108	.	terminator	1755	1883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124118
BBa_K1124108	.	terminator	1843	1883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124108	.	stem_loop	1850	1869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124108	.	engineered_region	1843	1883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124108	.	stop_codon	1876	1876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124108	.	polyA_site	1870	1883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124108	.	terminator	1755	1834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124108	.	engineered_region	1755	1834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124108	.	stem_loop	1766	1809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124108	.	CDS	1029	1746	.	+	0	ID=BBa_K1124003;Name=BBa_K1124003;Parent=BBa_K1124118
BBa_K1124108	.	stop_codon	1740	1742	.	+	0	ID=annotation2340390;Name=stop;Parent=BBa_K1124003
BBa_K1124108	.	point_mutation	1728	1728	.	+	0	ID=annotation2340454;Name=E234K (point mutation G to A);Parent=BBa_K1124003
BBa_K1124108	.	stop_codon	1743	1745	.	+	0	ID=annotation2340391;Name=stop;Parent=BBa_K1124003
BBa_K1124108	.	CDS	1029	1739	.	+	0	ID=annotation2340392;Name=lambda cI repressor (E234K);Parent=BBa_K1124003
BBa_K1124108	.	ribosome_entry_site	1011	1022	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124118
BBa_K1124108	.	conserved_region	1015	1018	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1124108
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaa
cggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgacc
aagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcacc
ccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacgg
cggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaac
ttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacg
gcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccaccta
caaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggac
tacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaa
acgacgaaaactacgctctagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaaaagacgtttgg
ctaataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata