BBa_K1124108
type: ComponentDefinition
persistent identity: BBa K1124108
display id: BBa_K1124108
version: 1
was generated by: iGEM to SBOL conversion
description: pLambda-mCherry-pConst-c1 repressor generator (-LVA, -barcode, E234K) (for UV induction check)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1124118
component: BBa_J23101
component: BBa_R0051
component: BBa_B0015
component: BBa_K773003
sequence annotation: BBa_R0051
sequence annotation: BBa_K773003
sequence annotation: BBa_B0015
sequence annotation: BBa_J23101
sequence annotation: BBa_K1124118
sequence: https://synbiohub.org/public/igem/BBa_K1124108_sequence/1

BBa_K1124118
type: Component
persistent identity: component2350781
display id: component2350781
version: 1
top level: BBa_K1124108
definition: BBa_K1124118
access: public

BBa_J23101
type: Component
persistent identity: component2350766
display id: component2350766
version: 1
top level: BBa_K1124108
definition: BBa_J23101
access: public

BBa_R0051
type: Component
persistent identity: component2350748
display id: component2350748
version: 1
top level: BBa_K1124108
definition: cI lam
access: public

BBa_B0015
type: Component
persistent identity: component2350765
display id: component2350765
version: 1
top level: BBa_K1124108
definition: BBa_B0015
access: public

BBa_K773003
type: Component
persistent identity: component2350758
display id: component2350758
version: 1
top level: BBa_K1124108
definition: BBa_K773003
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2350748
display id: annotation2350748
version: 1
top level: BBa_K1124108
location: https://synbiohub.org/public/igem/BBa_K1124108/annotation2350748/range2350748/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1124108/annotation2350748/range2350748/1
type: Range
persistent identity: range2350748
display id: range2350748
version: 1
top level: BBa_K1124108
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_K773003
type: SequenceAnnotation
persistent identity: annotation2350758
display id: annotation2350758
version: 1
top level: BBa_K1124108
location: https://synbiohub.org/public/igem/BBa_K1124108/annotation2350758/range2350758/1
component: BBa_K773003

https://synbiohub.org/public/igem/BBa_K1124108/annotation2350758/range2350758/1
type: Range
persistent identity: range2350758
display id: range2350758
version: 1
top level: BBa_K1124108
direction: direction/reverse
start: 58
end: 822
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2350765
display id: annotation2350765
version: 1
top level: BBa_K1124108
location: https://synbiohub.org/public/igem/BBa_K1124108/annotation2350765/range2350765/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124108/annotation2350765/range2350765/1
type: Range
persistent identity: range2350765
display id: range2350765
version: 1
top level: BBa_K1124108
direction: direction/reverse
start: 831
end: 959
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2350766
display id: annotation2350766
version: 1
top level: BBa_K1124108
location: https://synbiohub.org/public/igem/BBa_K1124108/annotation2350766/range2350766/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1124108/annotation2350766/range2350766/1
type: Range
persistent identity: range2350766
display id: range2350766
version: 1
top level: BBa_K1124108
direction: direction/reverse
start: 968
end: 1002
orientation: inline

BBa_K1124118
type: SequenceAnnotation
persistent identity: annotation2350781
display id: annotation2350781
version: 1
top level: BBa_K1124108
location: https://synbiohub.org/public/igem/BBa_K1124108/annotation2350781/range2350781/1
component: BBa_K1124118

https://synbiohub.org/public/igem/BBa_K1124108/annotation2350781/range2350781/1
type: Range
persistent identity: range2350781
display id: range2350781
version: 1
top level: BBa_K1124108
direction: direction/reverse
start: 1011
end: 1883
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K773003
type: ComponentDefinition
persistent identity: BBa K773003
display id: BBa_K773003
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry-LVA
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We modified the AAV sequence to LVA with PCR.
star: false
creator: Katie Knister
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: mCherry-LVA
sequence annotation: Start
sequence annotation: ssrA degradation tag: LVA
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K773003_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2209508
display id: annotation2209508
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
type: Range
persistent identity: range2209508
display id: range2209508
version: 1
top level: BBa_K773003
direction: direction/forward
start: 1
end: 12
orientation: inline

mCherry-LVA
type: SequenceAnnotation
persistent identity: annotation2209509
display id: annotation2209509
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
type: Range
persistent identity: range2209509
display id: range2209509
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 765
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209510
display id: annotation2209510
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
type: Range
persistent identity: range2209510
display id: range2209510
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 21
orientation: inline

ssrA degradation tag: LVA
type: SequenceAnnotation
persistent identity: annotation2209511
display id: annotation2209511
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
type: Range
persistent identity: range2209511
display id: range2209511
version: 1
top level: BBa_K773003
direction: direction/forward
start: 730
end: 759
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209512
display id: annotation2209512
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
type: Range
persistent identity: range2209512
display id: range2209512
version: 1
top level: BBa_K773003
direction: direction/forward
start: 760
end: 762
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209513
display id: annotation2209513
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
type: Range
persistent identity: range2209513
display id: range2209513
version: 1
top level: BBa_K773003
direction: direction/forward
start: 763
end: 765
orientation: inline

BBa_K1124118
type: ComponentDefinition
persistent identity: BBa K1124118
display id: BBa_K1124118
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor generator (-LVA, -barcode, E234K)(+RBS, +terminator+)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
experience: experience/None
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K1124003
sequence annotation: BBa_B0034
sequence annotation: BBa_K1124003
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124118_sequence/1

BBa_B0034
type: Component
persistent identity: component2341846
display id: component2341846
version: 1
top level: BBa_K1124118
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2341858
display id: component2341858
version: 1
top level: BBa_K1124118
definition: BBa_B0015
access: public

BBa_K1124003
type: Component
persistent identity: component2341851
display id: component2341851
version: 1
top level: BBa_K1124118
definition: BBa_K1124003
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2341846
display id: annotation2341846
version: 1
top level: BBa_K1124118
location: https://synbiohub.org/public/igem/BBa_K1124118/annotation2341846/range2341846/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1124118/annotation2341846/range2341846/1
type: Range
persistent identity: range2341846
display id: range2341846
version: 1
top level: BBa_K1124118
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1124003
type: SequenceAnnotation
persistent identity: annotation2341851
display id: annotation2341851
version: 1
top level: BBa_K1124118
location: https://synbiohub.org/public/igem/BBa_K1124118/annotation2341851/range2341851/1
component: BBa_K1124003

https://synbiohub.org/public/igem/BBa_K1124118/annotation2341851/range2341851/1
type: Range
persistent identity: range2341851
display id: range2341851
version: 1
top level: BBa_K1124118
direction: direction/reverse
start: 19
end: 736
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2341858
display id: annotation2341858
version: 1
top level: BBa_K1124118
location: https://synbiohub.org/public/igem/BBa_K1124118/annotation2341858/range2341858/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124118/annotation2341858/range2341858/1
type: Range
persistent identity: range2341858
display id: range2341858
version: 1
top level: BBa_K1124118
direction: direction/reverse
start: 745
end: 873
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_K1124003
type: ComponentDefinition
persistent identity: BBa K1124003
display id: BBa_K1124003
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor (-LVA, -barcode, E234K)
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: PCR
owned by: james
owned by: myers
mutable description: cI repressor (-LVA, -barcode, E234K)
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: PCR
star: false
creator: Yuta Otsuka
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: lambda cI repressor (E234K)
sequence annotation: E234K (point mutation G to A)
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1124003_sequence/1

lambda cI repressor (E234K)
type: SequenceAnnotation
persistent identity: annotation2340392
display id: annotation2340392
version: 1
top level: BBa_K1124003
location: https://synbiohub.org/public/igem/BBa_K1124003/annotation2340392/range2340392/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1124003/annotation2340392/range2340392/1
type: Range
persistent identity: range2340392
display id: range2340392
version: 1
top level: BBa_K1124003
direction: direction/forward
start: 1
end: 711
orientation: inline

E234K (point mutation G to A)
type: SequenceAnnotation
persistent identity: annotation2340454
display id: annotation2340454
version: 1
top level: BBa_K1124003
location: https://synbiohub.org/public/igem/BBa_K1124003/annotation2340454/range2340454/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K1124003/annotation2340454/range2340454/1
type: Range
persistent identity: range2340454
display id: range2340454
version: 1
top level: BBa_K1124003
direction: direction/forward
start: 700
end: 700
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2340390
display id: annotation2340390
version: 1
top level: BBa_K1124003
location: https://synbiohub.org/public/igem/BBa_K1124003/annotation2340390/range2340390/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1124003/annotation2340390/range2340390/1
type: Range
persistent identity: range2340390
display id: range2340390
version: 1
top level: BBa_K1124003
direction: direction/forward
start: 712
end: 714
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2340391
display id: annotation2340391
version: 1
top level: BBa_K1124003
location: https://synbiohub.org/public/igem/BBa_K1124003/annotation2340391/range2340391/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1124003/annotation2340391/range2340391/1
type: Range
persistent identity: range2340391
display id: range2340391
version: 1
top level: BBa_K1124003
direction: direction/forward
start: 715
end: 717
orientation: inline

https://synbiohub.org/public/igem/BBa_K1124108_sequence/1
type: Sequence
persistent identity: BBa K1124108 sequence
display id: BBa_K1124108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaaaagacgtttggctaataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K773003_sequence/1
type: Sequence
persistent identity: BBa K773003 sequence
display id: BBa_K773003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124118_sequence/1
type: Sequence
persistent identity: BBa K1124118 sequence
display id: BBa_K1124118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaaaagacgtttggctaataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124003_sequence/1
type: Sequence
persistent identity: BBa K1124003 sequence
display id: BBa_K1124003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaaaagacgtttggctaataat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z