LOCUS       BBa_K1124109            2052 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-mCherry-GFP-pLambda-sRNA(anti-mCherry) (for   sRNA mechanism 
            check)
ACCESSION   BBa_K1124109
VERSION     BBa_K1124109.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..75
                     /label=B0034
     CDS             82..828
                     /label=mCherry-LVA
     misc_feature    82..84
                     /label=Start
     misc_feature    793..822
                     /label=ssrA degradation tag: LVA
     misc_feature    823..825
                     /label=Stop
     misc_feature    826..828
                     /label=Stop
     misc_feature    64..828
                     /label=BBa_K773003
     misc_feature    841..844
                     /label=conserved
     RBS             837..848
                     /label=BBa_B0034
     misc_feature    855..1574
                     /label=GFP
     misc_feature    1575..1607
                     /label=LVA
     CDS             855..1610
                     /label=BBa_K082003
     stem_loop       1630..1673
                     /label=stem_loop
     misc_feature    1619..1698
                     /label=BBa_B0010
     terminator      1619..1698
                     /label=BBa_B0010
     stem_loop       1714..1733
                     /label=T7 TE
     misc_feature    1740..1740
                     /label=stop
     polyA_site      1734..1747
                     /label=polya
     misc_feature    1707..1747
                     /label=BBa_B0012
     terminator      1707..1747
                     /label=BBa_B0012
     terminator      1619..1747
                     /label=BBa_B0015
     promoter        1793..1798
                     /label=-10
     promoter        1770..1775
                     /label=-35
     misc_feature    1780..1796
                     /label=OR1
     misc_feature    1756..1772
                     /label=OR2
     misc_feature    1756..1804
                     /label=BBa_R0051
     promoter        1756..1804
                     /label=BBa_R0051
     misc_feature    1837..1915
                     /label=MicC scaffold
     misc_feature    1813..1836
                     /label=Target-binding sequence
     misc_feature    1813..1915
                     /label=BBa_K1124006
     stem_loop       1935..1978
                     /label=stem_loop
     misc_feature    1924..2003
                     /label=BBa_B0010
     terminator      1924..2003
                     /label=BBa_B0010
     stem_loop       2019..2038
                     /label=T7 TE
     misc_feature    2045..2045
                     /label=stop
     polyA_site      2039..2052
                     /label=polya
     misc_feature    2012..2052
                     /label=BBa_B0012
     terminator      2012..2052
                     /label=BBa_B0012
     terminator      1924..2052
                     /label=BBa_B0015
     misc_feature    1813..2052
                     /label=BBa_K1124113
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc
      121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      781 ctgtacaagg cagcaaacga cgaaaactac gctctagtag cttaataata ctagagaaag
      841 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      901 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      961 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     1021 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
     1081 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     1141 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     1201 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     1261 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     1321 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
     1381 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     1441 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     1501 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     1561 tatacaaaag gcctgctgca aacgacgaaa actacgcttt agtagcttaa tactagagcc
     1621 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1681 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1741 gtttatatac tagagtaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg
     1801 ttgctactag agatcctcct cgcccttgct caccattttc tgttgggcca ttgcattgcc
     1861 actgattttc caacatataa aaagacaagc ccgaacagtc gtccgggctt ttttttacta
     1921 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1981 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2041 tctgcgttta ta
//