##gff-version 3
##sequence-region BBa_K1124109 1 2052
BBa_K1124109	.	engineered_region	64	828	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124109	.	CDS	82	828	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124109	.	ribosome_entry_site	64	75	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124109	.	start_codon	82	84	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124109	.	stop_codon	823	825	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124109	.	stop_codon	826	828	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124109	.	tag	793	822	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124109	.	ribosome_entry_site	837	848	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1124109	.	conserved_region	841	844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124109	.	promoter	1756	1804	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K1124109	.	engineered_region	1756	1804	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1124109	.	promoter	1793	1798	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1124109	.	non_covalent_binding_site	1780	1796	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1124109	.	promoter	1770	1775	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1124109	.	non_covalent_binding_site	1756	1772	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1124109	.	terminator	1619	1747	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124109	.	terminator	1619	1698	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124109	.	engineered_region	1619	1698	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124109	.	stem_loop	1630	1673	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124109	.	terminator	1707	1747	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124109	.	stop_codon	1740	1740	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124109	.	polyA_site	1734	1747	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124109	.	stem_loop	1714	1733	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124109	.	engineered_region	1707	1747	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124109	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1124109	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1124109	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1124109	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1124109	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1124109	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1124109	.	CDS	855	1610	.	+	0	ID=BBa_K082003;Name=BBa_K082003
BBa_K1124109	.	engineered_region	855	1574	.	+	0	ID=annotation1992959;Name=GFP;Parent=BBa_K082003
BBa_K1124109	.	tag	1575	1607	.	+	0	ID=annotation1993327;Name=LVA;Parent=BBa_K082003
BBa_K1124109	.	engineered_region	1813	2052	.	+	0	ID=BBa_K1124113;Name=BBa_K1124113
BBa_K1124109	.	mature_transcript_region	1813	1915	.	+	0	ID=BBa_K1124006;Name=BBa_K1124006;Parent=BBa_K1124113
BBa_K1124109	.	sequence_feature	1813	1836	.	+	0	ID=annotation2339033;Name=Target-binding sequence;Parent=BBa_K1124006
BBa_K1124109	.	sequence_feature	1837	1915	.	+	0	ID=annotation2339032;Name=MicC scaffold;Parent=BBa_K1124006
BBa_K1124109	.	terminator	1924	2052	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124113
BBa_K1124109	.	terminator	1924	2003	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124109	.	engineered_region	1924	2003	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124109	.	stem_loop	1935	1978	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124109	.	terminator	2012	2052	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124109	.	stop_codon	2045	2045	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124109	.	polyA_site	2039	2052	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124109	.	stem_loop	2019	2038	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124109	.	engineered_region	2012	2052	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1124109
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctc
cgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaag
gtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtga
agcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcga
ggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggc
accaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccg
aggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagac
cacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaac
gaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagg
cagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagaggagaaatactagatgcgtaaaggagaa
gaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggag
agggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggcc
aacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttc
aagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctg
aagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattct
tggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtt
aacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcg
atggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagaga
ccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgca
aacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagatcct
cctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaac
agtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata