BBa_K1124109
type: ComponentDefinition
persistent identity: BBa K1124109
display id: BBa_K1124109
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-mCherry-GFP-pLambda-sRNA(anti-mCherry) (for sRNA mechanism check)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0011
component: BBa_K1124113
component: BBa_K082003
component: BBa_B0034
component: BBa_R0051
component: BBa_B0015
component: BBa_K773003
sequence annotation: BBa_R0011
sequence annotation: BBa_K773003
sequence annotation: BBa_B0034
sequence annotation: BBa_K082003
sequence annotation: BBa_B0015
sequence annotation: BBa_R0051
sequence annotation: BBa_K1124113
sequence: https://synbiohub.org/public/igem/BBa_K1124109_sequence/1

BBa_R0011
type: Component
persistent identity: component2350782
display id: component2350782
version: 1
top level: BBa_K1124109
definition: lacI+pL
access: public

BBa_K1124113
type: Component
persistent identity: component2350821
display id: component2350821
version: 1
top level: BBa_K1124109
definition: BBa_K1124113
access: public

BBa_K082003
type: Component
persistent identity: component2350798
display id: component2350798
version: 1
top level: BBa_K1124109
definition: BBa_K082003
access: public

BBa_B0034
type: Component
persistent identity: component2350795
display id: component2350795
version: 1
top level: BBa_K1124109
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component2350807
display id: component2350807
version: 1
top level: BBa_K1124109
definition: cI lam
access: public

BBa_B0015
type: Component
persistent identity: component2350805
display id: component2350805
version: 1
top level: BBa_K1124109
definition: BBa_B0015
access: public

BBa_K773003
type: Component
persistent identity: component2350793
display id: component2350793
version: 1
top level: BBa_K1124109
definition: BBa_K773003
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2350782
display id: annotation2350782
version: 1
top level: BBa_K1124109
location: https://synbiohub.org/public/igem/BBa_K1124109/annotation2350782/range2350782/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K1124109/annotation2350782/range2350782/1
type: Range
persistent identity: range2350782
display id: range2350782
version: 1
top level: BBa_K1124109
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K773003
type: SequenceAnnotation
persistent identity: annotation2350793
display id: annotation2350793
version: 1
top level: BBa_K1124109
location: https://synbiohub.org/public/igem/BBa_K1124109/annotation2350793/range2350793/1
component: BBa_K773003

https://synbiohub.org/public/igem/BBa_K1124109/annotation2350793/range2350793/1
type: Range
persistent identity: range2350793
display id: range2350793
version: 1
top level: BBa_K1124109
direction: direction/reverse
start: 64
end: 828
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2350795
display id: annotation2350795
version: 1
top level: BBa_K1124109
location: https://synbiohub.org/public/igem/BBa_K1124109/annotation2350795/range2350795/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1124109/annotation2350795/range2350795/1
type: Range
persistent identity: range2350795
display id: range2350795
version: 1
top level: BBa_K1124109
direction: direction/reverse
start: 837
end: 848
orientation: inline

BBa_K082003
type: SequenceAnnotation
persistent identity: annotation2350798
display id: annotation2350798
version: 1
top level: BBa_K1124109
location: https://synbiohub.org/public/igem/BBa_K1124109/annotation2350798/range2350798/1
component: BBa_K082003

https://synbiohub.org/public/igem/BBa_K1124109/annotation2350798/range2350798/1
type: Range
persistent identity: range2350798
display id: range2350798
version: 1
top level: BBa_K1124109
direction: direction/reverse
start: 855
end: 1610
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2350805
display id: annotation2350805
version: 1
top level: BBa_K1124109
location: https://synbiohub.org/public/igem/BBa_K1124109/annotation2350805/range2350805/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124109/annotation2350805/range2350805/1
type: Range
persistent identity: range2350805
display id: range2350805
version: 1
top level: BBa_K1124109
direction: direction/reverse
start: 1619
end: 1747
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2350807
display id: annotation2350807
version: 1
top level: BBa_K1124109
location: https://synbiohub.org/public/igem/BBa_K1124109/annotation2350807/range2350807/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K1124109/annotation2350807/range2350807/1
type: Range
persistent identity: range2350807
display id: range2350807
version: 1
top level: BBa_K1124109
direction: direction/forward
start: 1756
end: 1804
orientation: inline

BBa_K1124113
type: SequenceAnnotation
persistent identity: annotation2350821
display id: annotation2350821
version: 1
top level: BBa_K1124109
location: https://synbiohub.org/public/igem/BBa_K1124109/annotation2350821/range2350821/1
component: BBa_K1124113

https://synbiohub.org/public/igem/BBa_K1124109/annotation2350821/range2350821/1
type: Range
persistent identity: range2350821
display id: range2350821
version: 1
top level: BBa_K1124109
direction: direction/reverse
start: 1813
end: 2052
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K773003
type: ComponentDefinition
persistent identity: BBa K773003
display id: BBa_K773003
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry-LVA
created: 2012-09-30T11:00:00Z
modified: 2015-05-08T01:13:15Z
mutable provenance: This part was modified from a plasmid provided by the Murray lab at Caltech.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part contains the fluorescent protein mCherry attached to a degradation tag. It will decrease the amount of fluorescent protein over time.
discontinued: false
dominant: false
group u list: _1025_
m user id: 0
owner id: 11646
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We modified the AAV sequence to LVA with PCR.
star: false
creator: Katie Knister
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: mCherry-LVA
sequence annotation: Start
sequence annotation: ssrA degradation tag: LVA
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K773003_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation2209508
display id: annotation2209508
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K773003/annotation2209508/range2209508/1
type: Range
persistent identity: range2209508
display id: range2209508
version: 1
top level: BBa_K773003
direction: direction/forward
start: 1
end: 12
orientation: inline

mCherry-LVA
type: SequenceAnnotation
persistent identity: annotation2209509
display id: annotation2209509
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K773003/annotation2209509/range2209509/1
type: Range
persistent identity: range2209509
display id: range2209509
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 765
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation2209510
display id: annotation2209510
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K773003/annotation2209510/range2209510/1
type: Range
persistent identity: range2209510
display id: range2209510
version: 1
top level: BBa_K773003
direction: direction/forward
start: 19
end: 21
orientation: inline

ssrA degradation tag: LVA
type: SequenceAnnotation
persistent identity: annotation2209511
display id: annotation2209511
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K773003/annotation2209511/range2209511/1
type: Range
persistent identity: range2209511
display id: range2209511
version: 1
top level: BBa_K773003
direction: direction/forward
start: 730
end: 759
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209512
display id: annotation2209512
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K773003/annotation2209512/range2209512/1
type: Range
persistent identity: range2209512
display id: range2209512
version: 1
top level: BBa_K773003
direction: direction/forward
start: 760
end: 762
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2209513
display id: annotation2209513
version: 1
top level: BBa_K773003
location: https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K773003/annotation2209513/range2209513/1
type: Range
persistent identity: range2209513
display id: range2209513
version: 1
top level: BBa_K773003
direction: direction/forward
start: 763
end: 765
orientation: inline

BBa_K1124113
type: ComponentDefinition
persistent identity: BBa K1124113
display id: BBa_K1124113
version: 1
was generated by: iGEM to SBOL conversion
description: anti-mCherry sRNA (+termintor) (sRNA generator)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K1124006
sequence annotation: BBa_K1124006
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124113_sequence/1

BBa_B0015
type: Component
persistent identity: component2341908
display id: component2341908
version: 1
top level: BBa_K1124113
definition: BBa_B0015
access: public

BBa_K1124006
type: Component
persistent identity: component2341901
display id: component2341901
version: 1
top level: BBa_K1124113
definition: BBa_K1124006
access: public

BBa_K1124006
type: SequenceAnnotation
persistent identity: annotation2341901
display id: annotation2341901
version: 1
top level: BBa_K1124113
location: https://synbiohub.org/public/igem/BBa_K1124113/annotation2341901/range2341901/1
component: BBa_K1124006

https://synbiohub.org/public/igem/BBa_K1124113/annotation2341901/range2341901/1
type: Range
persistent identity: range2341901
display id: range2341901
version: 1
top level: BBa_K1124113
direction: direction/reverse
start: 1
end: 103
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2341908
display id: annotation2341908
version: 1
top level: BBa_K1124113
location: https://synbiohub.org/public/igem/BBa_K1124113/annotation2341908/range2341908/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124113/annotation2341908/range2341908/1
type: Range
persistent identity: range2341908
display id: range2341908
version: 1
top level: BBa_K1124113
direction: direction/reverse
start: 112
end: 240
orientation: inline

BBa_K082003
type: ComponentDefinition
persistent identity: BBa K082003
display id: BBa_K082003
version: 1
was generated by: iGEM to SBOL conversion
description: GFP(+LVA)
created: 2008-10-27T12:00:00Z
modified: 2016-01-25T04:43:13Z
mutable provenance: none
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: noen
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 4206
owner id: 1560
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: none
star: false
creator: Yanting Xue , He Shen
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP
sequence annotation: LVA
sequence: https://synbiohub.org/public/igem/BBa_K082003_sequence/1

GFP
type: SequenceAnnotation
persistent identity: annotation1992959
display id: annotation1992959
version: 1
top level: BBa_K082003
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K082003/annotation1992959/range1992959/1
type: Range
persistent identity: range1992959
display id: range1992959
version: 1
top level: BBa_K082003
direction: direction/forward
start: 1
end: 720
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1993327
display id: annotation1993327
version: 1
top level: BBa_K082003
location: https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K082003/annotation1993327/range1993327/1
type: Range
persistent identity: range1993327
display id: range1993327
version: 1
top level: BBa_K082003
direction: direction/forward
start: 721
end: 753
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K1124006
type: ComponentDefinition
persistent identity: BBa K1124006
display id: BBa_K1124006
version: 1
was generated by: iGEM to SBOL conversion
description: anti-mCherry sRNA
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: PCR
owned by: james
owned by: myers
mutable description: anti-mCherry sRNA
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13134
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: PCR
star: false
creator: Yuta Otsuka
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: Target-binding sequence
sequence annotation: MicC scaffold
sequence: https://synbiohub.org/public/igem/BBa_K1124006_sequence/1

Target-binding sequence
type: SequenceAnnotation
persistent identity: annotation2339033
display id: annotation2339033
version: 1
top level: BBa_K1124006
location: https://synbiohub.org/public/igem/BBa_K1124006/annotation2339033/range2339033/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1124006/annotation2339033/range2339033/1
type: Range
persistent identity: range2339033
display id: range2339033
version: 1
top level: BBa_K1124006
direction: direction/forward
start: 1
end: 24
orientation: inline

MicC scaffold
type: SequenceAnnotation
persistent identity: annotation2339032
display id: annotation2339032
version: 1
top level: BBa_K1124006
location: https://synbiohub.org/public/igem/BBa_K1124006/annotation2339032/range2339032/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1124006/annotation2339032/range2339032/1
type: Range
persistent identity: range2339032
display id: range2339032
version: 1
top level: BBa_K1124006
direction: direction/forward
start: 25
end: 103
orientation: inline

https://synbiohub.org/public/igem/BBa_K1124109_sequence/1
type: Sequence
persistent identity: BBa K1124109 sequence
display id: BBa_K1124109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagatcctcctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K773003_sequence/1
type: Sequence
persistent identity: BBa K773003 sequence
display id: BBa_K773003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124113_sequence/1
type: Sequence
persistent identity: BBa K1124113 sequence
display id: BBa_K1124113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctcctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K082003_sequence/1
type: Sequence
persistent identity: BBa K082003 sequence
display id: BBa_K082003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124006_sequence/1
type: Sequence
persistent identity: BBa K1124006 sequence
display id: BBa_K1124006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcctcctcgcccttgctcaccattttctgttgggccattgcattgccactgattttccaacatataaaaagacaagcccgaacagtcgtccgggcttttttt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z