LOCUS       BBa_K1124110             893 bp    DNA     linear       22-Dec-2024
DEFINITION  amilGFP(+LVA) generator(+RBS, +terminator)
ACCESSION   BBa_K1124110
VERSION     BBa_K1124110.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    754..756
                     /label=stop
     misc_feature    751..753
                     /label=stop
     misc_feature    718..750
                     /label=LVA degradation tag
     RBS             19..717
                     /label=amilGFP
     misc_feature    19..756
                     /label=BBa_K1124000
     stem_loop       776..819
                     /label=stem_loop
     misc_feature    765..844
                     /label=BBa_B0010
     terminator      765..844
                     /label=BBa_B0010
     stem_loop       860..879
                     /label=T7 TE
     misc_feature    886..886
                     /label=stop
     polyA_site      880..893
                     /label=polya
     misc_feature    853..893
                     /label=BBa_B0012
     terminator      853..893
                     /label=BBa_B0012
     terminator      765..893
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gtcttattca aagcatggca tcgtacaaga aatgaagacg
       61 aaataccata tggaaggcag tgtcaatggc catgaattta cgatcgaagg tgtaggaact
      121 gggtaccctt acgaagggaa acagatgtcc gaattagtga tcatcaagcc tgcgggaaaa
      181 ccccttccat tctcctttga catactgtca tcagtctttc aatatggaaa ccgttgcttc
      241 acaaagtacc cggcagacat gcctgactat ttcaagcaag cattcccaga tggaatgtca
      301 tatgaaaggt catttctatt tgaggatgga gcagttgcta cagccagctg gaacattcgt
      361 ctcgaaggaa attgcttcat ccacaaatcc atctttcatg gcgtaaactt tcccgctgat
      421 ggacccgtaa tgaaaaagaa gacaattgac tgggataagt ccttcgaaaa aatgactgtg
      481 tctaaagagg tgctaagagg tgacgtgact atgtttctta tgctcgaagg aggtggttct
      541 cacagatgcc aatttcactc cacttacaaa acagagaagc cggtcacact gcccccgaat
      601 catgtcgtag aacatcaaat tgtgaggacc gaccttggcc aaagtgcaaa aggctttaca
      661 gtcaagctgg aagcacatgc cgcggctcat gttaaccctt tgaaggttaa aaggcctgct
      721 gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc atcaaataaa
      781 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      841 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//