##gff-version 3
##sequence-region BBa_K1124200 1 3202
BBa_K1124200	.	promoter	1	72	.	+	0	ID=BBa_K176000;Name=BBa_K176000
BBa_K1124200	.	promoter	42	47	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124200	.	start_codon	53	53	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124200	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124200	.	non_covalent_binding_site	54	72	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124200	.	promoter	20	25	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124200	.	engineered_region	1	53	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124200	.	engineered_region	2233	3202	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124200	.	CDS	2252	3040	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124200	.	tag	3002	3034	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124200	.	start_codon	2252	2254	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124200	.	engineered_region	2252	3040	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124200	.	CDS	2252	3001	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124200	.	engineered_tag	3041	3065	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124200	.	stop_codon	3035	3040	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124200	.	ribosome_entry_site	2233	2245	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124200	.	ribosome_entry_site	2233	2245	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124200	.	ribosome_entry_site	2239	2242	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124200	.	engineered_region	2233	2245	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124200	.	terminator	3074	3202	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124200	.	terminator	3074	3153	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124200	.	stem_loop	3085	3128	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124200	.	engineered_region	3074	3153	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124200	.	terminator	3162	3202	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124200	.	stem_loop	3169	3188	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124200	.	stop_codon	3195	3195	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124200	.	engineered_region	3162	3202	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124200	.	polyA_site	3189	3202	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124200	.	engineered_region	1523	2224	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_K1124200	.	ribosome_entry_site	1523	1533	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124200	.	engineered_region	1523	1533	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124200	.	ribosome_entry_site	1523	1533	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124200	.	ribosome_entry_site	1529	1532	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124200	.	CDS	1540	2199	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124200	.	engineered_tag	2200	2224	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124200	.	CDS	1543	2159	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124200	.	tag	2160	2193	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1124200	.	engineered_region	854	1514	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K1124200	.	CDS	872	1489	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1124200	.	engineered_region	872	1489	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1124200	.	tag	1451	1482	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1124200	.	CDS	872	1450	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1124200	.	engineered_tag	1490	1514	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1124200	.	ribosome_entry_site	854	865	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1124200	.	conserved_region	858	861	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124200	.	engineered_region	81	845	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124200	.	stop_codon	840	842	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124200	.	CDS	99	845	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124200	.	ribosome_entry_site	81	92	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124200	.	stop_codon	843	845	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124200	.	tag	810	839	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124200	.	start_codon	99	101	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
>BBa_K1124200
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactaga
gaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaag
gtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccc
agaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacgg
ctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggag
cgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctaca
aggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctc
cgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactac
gacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgg
acatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcat
ggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagtcacacaggactactagatgtccagattagataaaagtaaagtgattaacagcgcatt
agagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctaca
ttgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcact
tttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtca
tcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagccttt
ttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtat
tggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgaca
agctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaa
aaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgct
agtgtagatcactactagagtcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgtt
gtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatctttt
ggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggt
acatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatg
agccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatac
accaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaat
ttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccag
ggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaa
ttttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtg
atgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatag
ctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttata