LOCUS       BBa_K1124204            2533 bp    DNA     linear       11-May-2024
DEFINITION  pLux/tet-mCherry-tetR-aiiA
ACCESSION   BBa_K1124204
VERSION     BBa_K1124204.1  
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /label=R0062
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    54..72
                     /label=tetR
     misc_feature    53..53
                     /label=+1
     misc_feature    1..20
                     /label=luxR/HSL
     promoter        1..72
                     /label=BBa_K176000
     RBS             81..92
                     /label=B0034
     CDS             99..845
                     /label=mCherry-LVA
     misc_feature    99..101
                     /label=Start
     misc_feature    810..839
                     /label=ssrA degradation tag: LVA
     misc_feature    840..842
                     /label=Stop
     misc_feature    843..845
                     /label=Stop
     misc_feature    81..845
                     /label=BBa_K773003
     RBS             854..864
                     /label=RBS-4\Weaker
     RBS             860..863
                     /label=RBS
     misc_feature    854..864
                     /label=BBa_B0033
     RBS             854..864
                     /label=BBa_B0033
     CDS             874..1490
                     /label=tetR
     misc_feature    1491..1524
                     /label=SsrA
     misc_feature    1531..1555
                     /label=Help:Barcodes
     CDS             871..1530
                     /label=BBa_C0040
     misc_feature    854..1555
                     /label=BBa_S0107
     RBS             1564..1576
                     /label=RBS-3\Weak
     RBS             1570..1573
                     /label=RBS
     misc_feature    1564..1576
                     /label=BBa_B0032
     RBS             1564..1576
                     /label=BBa_B0032
     misc_feature    1583..1585
                     /label=start
     misc_feature    2366..2371
                     /label=2
     misc_feature    2333..2365
                     /label=LVA
     CDS             1583..2332
                     /label=aiiA
     misc_feature    1583..2371
                     /label=BBa_C0060
     misc_feature    2372..2396
                     /label=Help:Barcodes
     CDS             1583..2371
                     /label=BBa_C0060
     stem_loop       2416..2459
                     /label=stem_loop
     misc_feature    2405..2484
                     /label=BBa_B0010
     terminator      2405..2484
                     /label=BBa_B0010
     stem_loop       2500..2519
                     /label=T7 TE
     misc_feature    2526..2526
                     /label=stop
     polyA_site      2520..2533
                     /label=polya
     misc_feature    2493..2533
                     /label=BBa_B0012
     terminator      2493..2533
                     /label=BBa_B0012
     terminator      2405..2533
                     /label=BBa_B0015
     misc_feature    1564..2533
                     /label=BBa_K516022
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat
       61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
      121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
      481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt
      841 aataatacta gagtcacaca ggactactag atgtccagat tagataaaag taaagtgatt
      901 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
      961 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     1021 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     1081 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     1141 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     1201 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     1261 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     1321 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     1381 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     1441 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac
     1501 gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta
     1561 gagtcacaca ggaaagtact agatgacagt aaagaagctt tatttcgtcc cagcaggtcg
     1621 ttgtatgttg gatcattcgt ctgttaatag tacattaaca ccaggagaat tattagactt
     1681 accggtttgg tgttatcttt tggagactga agaaggacct attttagtag atacaggtat
     1741 gccagaaagt gcagttaata atgaaggtct ttttaacggt acatttgtcg aagggcaggt
     1801 tttaccgaaa atgactgaag aagatagaat cgtgaatatt ttaaaacggg ttggttatga
     1861 gccggaagac cttctttata ttattagttc tcacttgcat tttgatcatg caggaggaaa
     1921 tggcgctttt ataaatacac caatcattgt acagcgtgct gaatatgagg cggcgcagca
     1981 tagcgaagaa tatttgaaag aatgtatatt gccgaattta aactacaaaa tcattgaagg
     2041 tgattatgaa gtcgtaccag gagttcaatt attgcataca ccaggccata ctccagggca
     2101 tcaatcgcta ttaattgaga cagaaaaatc cggtcctgta ttattaacga ttgatgcatc
     2161 gtatacgaaa gagaattttg aaaatgaagt gccatttgcg ggatttgatt cagaattagc
     2221 tttatcttca attaaacgtt taaaagaagt ggtgatgaaa gagaagccga ttgttttctt
     2281 tggacatgat atagagcagg aaaggggatg taaagtgttc cctgaatata tagctgcaaa
     2341 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
     2401 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     2461 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2521 ttctgcgttt ata
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