##gff-version 3
##sequence-region BBa_K1124204 1 2533
BBa_K1124204	.	engineered_region	854	1555	.	+	0	ID=BBa_S0107;Name=BBa_S0107
BBa_K1124204	.	ribosome_entry_site	854	864	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_S0107
BBa_K1124204	.	ribosome_entry_site	860	863	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124204	.	ribosome_entry_site	854	864	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124204	.	engineered_region	854	864	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124204	.	CDS	871	1530	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_S0107
BBa_K1124204	.	engineered_tag	1531	1555	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124204	.	tag	1491	1524	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1124204	.	CDS	874	1490	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124204	.	engineered_region	81	845	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124204	.	stop_codon	843	845	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124204	.	start_codon	99	101	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124204	.	stop_codon	840	842	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124204	.	CDS	99	845	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124204	.	tag	810	839	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124204	.	ribosome_entry_site	81	92	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124204	.	promoter	1	72	.	+	0	ID=BBa_K176000;Name=BBa_K176000
BBa_K1124204	.	non_covalent_binding_site	54	72	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124204	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124204	.	promoter	20	25	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124204	.	promoter	42	47	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124204	.	start_codon	53	53	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124204	.	engineered_region	1	53	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124204	.	engineered_region	1564	2533	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124204	.	ribosome_entry_site	1564	1576	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124204	.	ribosome_entry_site	1570	1573	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124204	.	engineered_region	1564	1576	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124204	.	ribosome_entry_site	1564	1576	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124204	.	terminator	2405	2533	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124204	.	terminator	2405	2484	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124204	.	stem_loop	2416	2459	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124204	.	engineered_region	2405	2484	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124204	.	terminator	2493	2533	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124204	.	engineered_region	2493	2533	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124204	.	stop_codon	2526	2526	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124204	.	stem_loop	2500	2519	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124204	.	polyA_site	2520	2533	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124204	.	CDS	1583	2371	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124204	.	stop_codon	2366	2371	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124204	.	start_codon	1583	1585	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124204	.	engineered_region	1583	2371	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124204	.	engineered_tag	2372	2396	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124204	.	tag	2333	2365	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124204	.	CDS	1583	2332	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
>BBa_K1124204
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactaga
gaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaag
gtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccc
agaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacgg
ctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggag
cgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctaca
aggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctc
cgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactac
gacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgg
acatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcat
ggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagtcacacaggactacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtcacacaggaaagtact
agatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaac
accaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggt
atgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactg
aagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcactt
gcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcg
cagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcg
taccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccgg
tcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattca
gaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgata
tagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaata
acgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttata