LOCUS       BBa_K1124205            1692 bp    DNA     linear       22-Dec-2024
DEFINITION  pLux/tet-mCherry-tetR
ACCESSION   BBa_K1124205
VERSION     BBa_K1124205.1  
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /label=R0062
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    54..72
                     /label=tetR
     misc_feature    53..53
                     /label=+1
     misc_feature    1..20
                     /label=luxR/HSL
     promoter        1..72
                     /label=BBa_K176000
     RBS             81..92
                     /label=B0034
     CDS             99..845
                     /label=mCherry-LVA
     misc_feature    99..101
                     /label=Start
     misc_feature    810..839
                     /label=ssrA degradation tag: LVA
     misc_feature    840..842
                     /label=Stop
     misc_feature    843..845
                     /label=Stop
     misc_feature    81..845
                     /label=BBa_K773003
     RBS             854..864
                     /label=RBS-4\Weaker
     RBS             860..863
                     /label=RBS
     misc_feature    854..864
                     /label=BBa_B0033
     RBS             854..864
                     /label=BBa_B0033
     CDS             874..1490
                     /label=tetR
     misc_feature    1491..1524
                     /label=SsrA
     misc_feature    1531..1555
                     /label=Help:Barcodes
     CDS             871..1530
                     /label=BBa_C0040
     stem_loop       1575..1618
                     /label=stem_loop
     misc_feature    1564..1643
                     /label=BBa_B0010
     terminator      1564..1643
                     /label=BBa_B0010
     stem_loop       1659..1678
                     /label=T7 TE
     misc_feature    1685..1685
                     /label=stop
     polyA_site      1679..1692
                     /label=polya
     misc_feature    1652..1692
                     /label=BBa_B0012
     terminator      1652..1692
                     /label=BBa_B0012
     misc_feature    854..1692
                     /label=BBa_P0340
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat
       61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
      121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
      481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt
      841 aataatacta gagtcacaca ggactactag atgtccagat tagataaaag taaagtgatt
      901 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
      961 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     1021 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     1081 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     1141 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     1201 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     1261 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     1321 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     1381 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     1441 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac
     1501 gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta
     1561 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1621 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1681 tctgcgttta ta
//