##gff-version 3
##sequence-region BBa_K1124205 1 1692
BBa_K1124205	.	engineered_region	854	1692	.	+	0	ID=BBa_P0340;Name=BBa_P0340
BBa_K1124205	.	terminator	1652	1692	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0340
BBa_K1124205	.	stop_codon	1685	1685	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124205	.	polyA_site	1679	1692	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124205	.	stem_loop	1659	1678	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124205	.	engineered_region	1652	1692	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124205	.	terminator	1564	1643	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0340
BBa_K1124205	.	engineered_region	1564	1643	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124205	.	stem_loop	1575	1618	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124205	.	ribosome_entry_site	854	864	.	+	0	ID=BBa_B0033;Name=BBa_B0033;Parent=BBa_P0340
BBa_K1124205	.	ribosome_entry_site	854	864	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K1124205	.	engineered_region	854	864	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K1124205	.	ribosome_entry_site	860	863	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K1124205	.	CDS	871	1530	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0340
BBa_K1124205	.	engineered_tag	1531	1555	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1124205	.	CDS	874	1490	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1124205	.	tag	1491	1524	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1124205	.	promoter	1	72	.	+	0	ID=BBa_K176000;Name=BBa_K176000
BBa_K1124205	.	promoter	20	25	.	+	0	ID=annotation2002608;Name=-35;Parent=BBa_K176000
BBa_K1124205	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2002612;Name=luxR/HSL;Parent=BBa_K176000
BBa_K1124205	.	promoter	42	47	.	+	0	ID=annotation2002609;Name=-10;Parent=BBa_K176000
BBa_K1124205	.	non_covalent_binding_site	54	72	.	+	0	ID=annotation2002610;Name=tetR;Parent=BBa_K176000
BBa_K1124205	.	start_codon	53	53	.	+	0	ID=annotation2002611;Name=+1;Parent=BBa_K176000
BBa_K1124205	.	engineered_region	1	53	.	+	0	ID=annotation2002607;Name=R0062;Parent=BBa_K176000
BBa_K1124205	.	engineered_region	81	845	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124205	.	CDS	99	845	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124205	.	ribosome_entry_site	81	92	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124205	.	start_codon	99	101	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124205	.	stop_codon	840	842	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124205	.	stop_codon	843	845	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124205	.	tag	810	839	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
>BBa_K1124205
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatatccctatcagtgatagagatactaga
gaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaag
gtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcaccc
agaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacgg
ctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggag
cgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctaca
aggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctc
cgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactac
gacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttgg
acatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcat
ggacgagctgtacaaggcagcaaacgacgaaaactacgctctagtagcttaataatactagagtcacacaggactacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttata