LOCUS       BBa_K1124206            1823 bp    DNA     linear       22-Dec-2024
DEFINITION  pLux/tet-mCherry-aiiA
ACCESSION   BBa_K1124206
VERSION     BBa_K1124206.1  
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /label=R0062
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    54..72
                     /label=tetR
     misc_feature    53..53
                     /label=+1
     misc_feature    1..20
                     /label=luxR/HSL
     promoter        1..72
                     /label=BBa_K176000
     RBS             81..92
                     /label=B0034
     CDS             99..845
                     /label=mCherry-LVA
     misc_feature    99..101
                     /label=Start
     misc_feature    810..839
                     /label=ssrA degradation tag: LVA
     misc_feature    840..842
                     /label=Stop
     misc_feature    843..845
                     /label=Stop
     misc_feature    81..845
                     /label=BBa_K773003
     RBS             854..866
                     /label=RBS-3\Weak
     RBS             860..863
                     /label=RBS
     misc_feature    854..866
                     /label=BBa_B0032
     RBS             854..866
                     /label=BBa_B0032
     misc_feature    873..875
                     /label=start
     misc_feature    1656..1661
                     /label=2
     misc_feature    1623..1655
                     /label=LVA
     CDS             873..1622
                     /label=aiiA
     misc_feature    873..1661
                     /label=BBa_C0060
     misc_feature    1662..1686
                     /label=Help:Barcodes
     CDS             873..1661
                     /label=BBa_C0060
     stem_loop       1706..1749
                     /label=stem_loop
     misc_feature    1695..1774
                     /label=BBa_B0010
     terminator      1695..1774
                     /label=BBa_B0010
     stem_loop       1790..1809
                     /label=T7 TE
     misc_feature    1816..1816
                     /label=stop
     polyA_site      1810..1823
                     /label=polya
     misc_feature    1783..1823
                     /label=BBa_B0012
     terminator      1783..1823
                     /label=BBa_B0012
     terminator      1695..1823
                     /label=BBa_B0015
     misc_feature    854..1823
                     /label=BBa_K516022
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat
       61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
      121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
      481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt
      841 aataatacta gagtcacaca ggaaagtact agatgacagt aaagaagctt tatttcgtcc
      901 cagcaggtcg ttgtatgttg gatcattcgt ctgttaatag tacattaaca ccaggagaat
      961 tattagactt accggtttgg tgttatcttt tggagactga agaaggacct attttagtag
     1021 atacaggtat gccagaaagt gcagttaata atgaaggtct ttttaacggt acatttgtcg
     1081 aagggcaggt tttaccgaaa atgactgaag aagatagaat cgtgaatatt ttaaaacggg
     1141 ttggttatga gccggaagac cttctttata ttattagttc tcacttgcat tttgatcatg
     1201 caggaggaaa tggcgctttt ataaatacac caatcattgt acagcgtgct gaatatgagg
     1261 cggcgcagca tagcgaagaa tatttgaaag aatgtatatt gccgaattta aactacaaaa
     1321 tcattgaagg tgattatgaa gtcgtaccag gagttcaatt attgcataca ccaggccata
     1381 ctccagggca tcaatcgcta ttaattgaga cagaaaaatc cggtcctgta ttattaacga
     1441 ttgatgcatc gtatacgaaa gagaattttg aaaatgaagt gccatttgcg ggatttgatt
     1501 cagaattagc tttatcttca attaaacgtt taaaagaagt ggtgatgaaa gagaagccga
     1561 ttgttttctt tggacatgat atagagcagg aaaggggatg taaagtgttc cctgaatata
     1621 tagctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta
     1681 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1741 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1801 gggtgggcct ttctgcgttt ata
//