LOCUS       BBa_K1124208            2475 bp    DNA     linear       22-Dec-2024
DEFINITION  pLux-mCherry-luxI-aiiA
ACCESSION   BBa_K1124208
VERSION     BBa_K1124208.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..75
                     /label=B0034
     CDS             82..828
                     /label=mCherry-LVA
     misc_feature    82..84
                     /label=Start
     misc_feature    793..822
                     /label=ssrA degradation tag: LVA
     misc_feature    823..825
                     /label=Stop
     misc_feature    826..828
                     /label=Stop
     misc_feature    64..828
                     /label=BBa_K773003
     misc_feature    841..844
                     /label=conserved
     RBS             837..848
                     /label=BBa_B0034
     misc_feature    1434..1465
                     /label=LVA
     CDS             855..1433
                     /label=luxI
     misc_feature    855..1472
                     /label=BBa_C0061
     misc_feature    1473..1497
                     /label=Help:Barcodes
     CDS             855..1472
                     /label=BBa_C0061
     misc_feature    837..1497
                     /label=BBa_C0261
     RBS             1506..1518
                     /label=RBS-3\Weak
     RBS             1512..1515
                     /label=RBS
     misc_feature    1506..1518
                     /label=BBa_B0032
     RBS             1506..1518
                     /label=BBa_B0032
     misc_feature    1525..1527
                     /label=start
     misc_feature    2308..2313
                     /label=2
     misc_feature    2275..2307
                     /label=LVA
     CDS             1525..2274
                     /label=aiiA
     misc_feature    1525..2313
                     /label=BBa_C0060
     misc_feature    2314..2338
                     /label=Help:Barcodes
     CDS             1525..2313
                     /label=BBa_C0060
     stem_loop       2358..2401
                     /label=stem_loop
     misc_feature    2347..2426
                     /label=BBa_B0010
     terminator      2347..2426
                     /label=BBa_B0010
     stem_loop       2442..2461
                     /label=T7 TE
     misc_feature    2468..2468
                     /label=stop
     polyA_site      2462..2475
                     /label=polya
     misc_feature    2435..2475
                     /label=BBa_B0012
     terminator      2435..2475
                     /label=BBa_B0012
     terminator      2347..2475
                     /label=BBa_B0015
     misc_feature    1506..2475
                     /label=BBa_K516022
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc
      121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      781 ctgtacaagg cagcaaacga cgaaaactac gctctagtag cttaataata ctagagaaag
      841 aggagaaata ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg
      901 aggagtataa aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg
      961 acttagttgt agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata
     1021 tttatgcttg tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag
     1081 gtgattatat gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag
     1141 atcctaatat agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata
     1201 actctgctag tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc
     1261 aaggtattac agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta
     1321 ttaaagttcc ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat
     1381 cggttgtatt gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa
     1441 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac
     1501 tagagtcaca caggaaagta ctagatgaca gtaaagaagc tttatttcgt cccagcaggt
     1561 cgttgtatgt tggatcattc gtctgttaat agtacattaa caccaggaga attattagac
     1621 ttaccggttt ggtgttatct tttggagact gaagaaggac ctattttagt agatacaggt
     1681 atgccagaaa gtgcagttaa taatgaaggt ctttttaacg gtacatttgt cgaagggcag
     1741 gttttaccga aaatgactga agaagataga atcgtgaata ttttaaaacg ggttggttat
     1801 gagccggaag accttcttta tattattagt tctcacttgc attttgatca tgcaggagga
     1861 aatggcgctt ttataaatac accaatcatt gtacagcgtg ctgaatatga ggcggcgcag
     1921 catagcgaag aatatttgaa agaatgtata ttgccgaatt taaactacaa aatcattgaa
     1981 ggtgattatg aagtcgtacc aggagttcaa ttattgcata caccaggcca tactccaggg
     2041 catcaatcgc tattaattga gacagaaaaa tccggtcctg tattattaac gattgatgca
     2101 tcgtatacga aagagaattt tgaaaatgaa gtgccatttg cgggatttga ttcagaatta
     2161 gctttatctt caattaaacg tttaaaagaa gtggtgatga aagagaagcc gattgttttc
     2221 tttggacatg atatagagca ggaaagggga tgtaaagtgt tccctgaata tatagctgca
     2281 aacgacgaaa actacgcttt agtagcttaa taacgctgat agtgctagtg tagatcgcta
     2341 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2401 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2461 ctttctgcgt ttata
//