##gff-version 3
##sequence-region BBa_K1124208 1 2475
BBa_K1124208	.	engineered_region	837	1497	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K1124208	.	CDS	855	1472	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1124208	.	engineered_region	855	1472	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1124208	.	engineered_tag	1473	1497	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1124208	.	CDS	855	1433	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1124208	.	tag	1434	1465	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1124208	.	ribosome_entry_site	837	848	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1124208	.	conserved_region	841	844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124208	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1124208	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1124208	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1124208	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1124208	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1124208	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1124208	.	engineered_region	1506	2475	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124208	.	ribosome_entry_site	1506	1518	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124208	.	engineered_region	1506	1518	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124208	.	ribosome_entry_site	1512	1515	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124208	.	ribosome_entry_site	1506	1518	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124208	.	CDS	1525	2313	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124208	.	tag	2275	2307	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124208	.	engineered_tag	2314	2338	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124208	.	CDS	1525	2274	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124208	.	engineered_region	1525	2313	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124208	.	start_codon	1525	1527	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124208	.	stop_codon	2308	2313	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124208	.	terminator	2347	2475	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124208	.	terminator	2435	2475	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124208	.	stem_loop	2442	2461	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124208	.	polyA_site	2462	2475	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124208	.	stop_codon	2468	2468	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124208	.	engineered_region	2435	2475	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124208	.	terminator	2347	2426	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124208	.	stem_loop	2358	2401	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124208	.	engineered_region	2347	2426	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124208	.	engineered_region	64	828	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124208	.	stop_codon	826	828	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124208	.	CDS	82	828	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124208	.	tag	793	822	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124208	.	start_codon	82	84	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124208	.	stop_codon	823	825	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124208	.	ribosome_entry_site	64	75	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
>BBa_K1124208
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctc
cgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaag
gtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtga
agcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcga
ggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggc
accaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccg
aggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagac
cacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaac
gaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagg
cagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagaggagaaatactagatgactataatgata
aaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaa
gacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgc
ttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgttttt
cctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaata
gctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaagg
tattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtatt
ggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaag
cagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctact
agagtcacacaggaaagtactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattc
gtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaagga
cctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggc
aggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttct
ttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacag
cgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatca
ttgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctatt
aattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtg
ccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccga
ttgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaa
ctacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttata