LOCUS       BBa_K1124209            1634 bp    DNA     linear       22-Nov-2024
DEFINITION  pLux-mCherry-luxI
ACCESSION   BBa_K1124209
VERSION     BBa_K1124209.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..75
                     /label=B0034
     CDS             82..828
                     /label=mCherry-LVA
     misc_feature    82..84
                     /label=Start
     misc_feature    793..822
                     /label=ssrA degradation tag: LVA
     misc_feature    823..825
                     /label=Stop
     misc_feature    826..828
                     /label=Stop
     misc_feature    64..828
                     /label=BBa_K773003
     misc_feature    841..844
                     /label=conserved
     RBS             837..848
                     /label=BBa_B0034
     misc_feature    1434..1465
                     /label=LVA
     CDS             855..1433
                     /label=luxI
     misc_feature    855..1472
                     /label=BBa_C0061
     misc_feature    1473..1497
                     /label=Help:Barcodes
     CDS             855..1472
                     /label=BBa_C0061
     misc_feature    837..1497
                     /label=BBa_C0261
     stem_loop       1517..1560
                     /label=stem_loop
     misc_feature    1506..1585
                     /label=BBa_B0010
     terminator      1506..1585
                     /label=BBa_B0010
     stem_loop       1601..1620
                     /label=T7 TE
     misc_feature    1627..1627
                     /label=stop
     polyA_site      1621..1634
                     /label=polya
     misc_feature    1594..1634
                     /label=BBa_B0012
     terminator      1594..1634
                     /label=BBa_B0012
     terminator      1506..1634
                     /label=BBa_B0015
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc
      121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      781 ctgtacaagg cagcaaacga cgaaaactac gctctagtag cttaataata ctagagaaag
      841 aggagaaata ctagatgact ataatgataa aaaaatcgga ttttttggca attccatcgg
      901 aggagtataa aggtattcta agtcttcgtt atcaagtgtt taagcaaaga cttgagtggg
      961 acttagttgt agaaaataac cttgaatcag atgagtatga taactcaaat gcagaatata
     1021 tttatgcttg tgatgatact gaaaatgtaa gtggatgctg gcgtttatta cctacaacag
     1081 gtgattatat gctgaaaagt gtttttcctg aattgcttgg tcaacagagt gctcccaaag
     1141 atcctaatat agtcgaatta agtcgttttg ctgtaggtaa aaatagctca aagataaata
     1201 actctgctag tgaaattaca atgaaactat ttgaagctat atataaacac gctgttagtc
     1261 aaggtattac agaatatgta acagtaacat caacagcaat agagcgattt ttaaagcgta
     1321 ttaaagttcc ttgtcatcgt attggagaca aagaaattca tgtattaggt gatactaaat
     1381 cggttgtatt gtctatgcct attaatgaac agtttaaaaa agcagtctta aatgctgcaa
     1441 acgacgaaaa ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac
     1501 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1561 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1621 tttctgcgtt tata
//