##gff-version 3
##sequence-region BBa_K1124209 1 1634
BBa_K1124209	.	engineered_region	64	828	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124209	.	ribosome_entry_site	64	75	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124209	.	CDS	82	828	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124209	.	stop_codon	826	828	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124209	.	tag	793	822	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124209	.	stop_codon	823	825	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124209	.	start_codon	82	84	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124209	.	engineered_region	837	1497	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K1124209	.	ribosome_entry_site	837	848	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1124209	.	conserved_region	841	844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124209	.	CDS	855	1472	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1124209	.	CDS	855	1433	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1124209	.	engineered_tag	1473	1497	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1124209	.	tag	1434	1465	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1124209	.	engineered_region	855	1472	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1124209	.	terminator	1506	1634	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1124209	.	terminator	1594	1634	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124209	.	engineered_region	1594	1634	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124209	.	stem_loop	1601	1620	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124209	.	stop_codon	1627	1627	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124209	.	polyA_site	1621	1634	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124209	.	terminator	1506	1585	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124209	.	engineered_region	1506	1585	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124209	.	stem_loop	1517	1560	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124209	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1124209	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1124209	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1124209	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1124209	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1124209	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
>BBa_K1124209
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctc
cgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaag
gtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtga
agcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcga
ggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggc
accaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccg
aggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagac
cacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaac
gaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagg
cagcaaacgacgaaaactacgctctagtagcttaataatactagagaaagaggagaaatactagatgactataatgata
aaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaa
gacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgc
ttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgttttt
cctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaata
gctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaagg
tattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtatt
ggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaag
cagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata