LOCUS       BBa_K1124210            1806 bp    DNA     linear       17-May-2024
DEFINITION  pLux-mCherry-aiiA
ACCESSION   BBa_K1124210
VERSION     BBa_K1124210.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             64..75
                     /label=B0034
     CDS             82..828
                     /label=mCherry-LVA
     misc_feature    82..84
                     /label=Start
     misc_feature    793..822
                     /label=ssrA degradation tag: LVA
     misc_feature    823..825
                     /label=Stop
     misc_feature    826..828
                     /label=Stop
     misc_feature    64..828
                     /label=BBa_K773003
     RBS             837..849
                     /label=RBS-3\Weak
     RBS             843..846
                     /label=RBS
     misc_feature    837..849
                     /label=BBa_B0032
     RBS             837..849
                     /label=BBa_B0032
     misc_feature    856..858
                     /label=start
     misc_feature    1639..1644
                     /label=2
     misc_feature    1606..1638
                     /label=LVA
     CDS             856..1605
                     /label=aiiA
     misc_feature    856..1644
                     /label=BBa_C0060
     misc_feature    1645..1669
                     /label=Help:Barcodes
     CDS             856..1644
                     /label=BBa_C0060
     stem_loop       1689..1732
                     /label=stem_loop
     misc_feature    1678..1757
                     /label=BBa_B0010
     terminator      1678..1757
                     /label=BBa_B0010
     stem_loop       1773..1792
                     /label=T7 TE
     misc_feature    1799..1799
                     /label=stop
     polyA_site      1793..1806
                     /label=polya
     misc_feature    1766..1806
                     /label=BBa_B0012
     terminator      1766..1806
                     /label=BBa_B0012
     terminator      1678..1806
                     /label=BBa_B0015
     misc_feature    837..1806
                     /label=BBa_K516022
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc
      121 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      181 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      241 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      301 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      361 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      421 caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      481 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      541 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      601 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      661 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      721 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      781 ctgtacaagg cagcaaacga cgaaaactac gctctagtag cttaataata ctagagtcac
      841 acaggaaagt actagatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg
      901 ttggatcatt cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt
      961 tggtgttatc ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa
     1021 agtgcagtta ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg
     1081 aaaatgactg aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa
     1141 gaccttcttt atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct
     1201 tttataaata caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa
     1261 gaatatttga aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat
     1321 gaagtcgtac caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg
     1381 ctattaattg agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg
     1441 aaagagaatt ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct
     1501 tcaattaaac gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat
     1561 gatatagagc aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa
     1621 aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca
     1681 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1741 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1801 tttata
//