##gff-version 3
##sequence-region BBa_K1124210 1 1806
BBa_K1124210	.	engineered_region	64	828	.	+	0	ID=BBa_K773003;Name=BBa_K773003
BBa_K1124210	.	CDS	82	828	.	+	0	ID=annotation2209509;Name=mCherry-LVA;Parent=BBa_K773003
BBa_K1124210	.	ribosome_entry_site	64	75	.	+	0	ID=annotation2209508;Name=B0034;Parent=BBa_K773003
BBa_K1124210	.	start_codon	82	84	.	+	0	ID=annotation2209510;Name=Start;Parent=BBa_K773003
BBa_K1124210	.	stop_codon	823	825	.	+	0	ID=annotation2209512;Name=Stop;Parent=BBa_K773003
BBa_K1124210	.	stop_codon	826	828	.	+	0	ID=annotation2209513;Name=Stop;Parent=BBa_K773003
BBa_K1124210	.	tag	793	822	.	+	0	ID=annotation2209511;Name=ssrA degradation tag: LVA;Parent=BBa_K773003
BBa_K1124210	.	engineered_region	837	1806	.	+	0	ID=BBa_K516022;Name=BBa_K516022
BBa_K1124210	.	CDS	856	1644	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_K516022
BBa_K1124210	.	tag	1606	1638	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K1124210	.	stop_codon	1639	1644	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K1124210	.	CDS	856	1605	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K1124210	.	engineered_region	856	1644	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K1124210	.	start_codon	856	858	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K1124210	.	engineered_tag	1645	1669	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K1124210	.	terminator	1678	1806	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516022
BBa_K1124210	.	terminator	1678	1757	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124210	.	engineered_region	1678	1757	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124210	.	stem_loop	1689	1732	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124210	.	terminator	1766	1806	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124210	.	stop_codon	1799	1799	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124210	.	polyA_site	1793	1806	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124210	.	stem_loop	1773	1792	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124210	.	engineered_region	1766	1806	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124210	.	ribosome_entry_site	837	849	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K516022
BBa_K1124210	.	ribosome_entry_site	837	849	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1124210	.	engineered_region	837	849	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1124210	.	ribosome_entry_site	843	846	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1124210	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1124210	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1124210	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1124210	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1124210	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1124210	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_K1124210
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctc
cgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaag
gtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtga
agcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcga
ggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggc
accaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccg
aggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagac
cacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaac
gaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagg
cagcaaacgacgaaaactacgctctagtagcttaataatactagagtcacacaggaaagtactagatgacagtaaagaa
gctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattatta
gacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcag
ttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgt
gaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgca
ggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaat
atttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaatt
attgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacg
attgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatctt
caattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaagggg
atgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctag
tgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata