LOCUS       BBa_K1124213            1923 bp    DNA     linear       26-Nov-2024
DEFINITION  Pconst (strong)-luxR-Pconst (strong)-amilGFP-LVA
ACCESSION   BBa_K1124213
VERSION     BBa_K1124213.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     terminator      851..979
                     /label=BBa_B0015
     misc_feature    1..979
                     /label=BBa_K1124212
     promoter        988..1022
                     /label=BBa_J23101
     misc_feature    1035..1038
                     /label=conserved
     RBS             1031..1042
                     /label=BBa_B0034
     misc_feature    1784..1786
                     /label=stop
     misc_feature    1781..1783
                     /label=stop
     misc_feature    1748..1780
                     /label=LVA degradation tag
     RBS             1049..1747
                     /label=amilGFP
     misc_feature    1049..1786
                     /label=BBa_K1124000
     stem_loop       1806..1849
                     /label=stem_loop
     misc_feature    1795..1874
                     /label=BBa_B0010
     terminator      1795..1874
                     /label=BBa_B0010
     stem_loop       1890..1909
                     /label=T7 TE
     misc_feature    1916..1916
                     /label=stop
     polyA_site      1910..1923
                     /label=polya
     misc_feature    1883..1923
                     /label=BBa_B0012
     terminator      1883..1923
                     /label=BBa_B0012
     terminator      1795..1923
                     /label=BBa_B0015
     misc_feature    1031..1923
                     /label=BBa_K1124110
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagttt acagctagct cagtcctagg tattatgcta
     1021 gctactagag aaagaggaga aatactagat gtcttattca aagcatggca tcgtacaaga
     1081 aatgaagacg aaataccata tggaaggcag tgtcaatggc catgaattta cgatcgaagg
     1141 tgtaggaact gggtaccctt acgaagggaa acagatgtcc gaattagtga tcatcaagcc
     1201 tgcgggaaaa ccccttccat tctcctttga catactgtca tcagtctttc aatatggaaa
     1261 ccgttgcttc acaaagtacc cggcagacat gcctgactat ttcaagcaag cattcccaga
     1321 tggaatgtca tatgaaaggt catttctatt tgaggatgga gcagttgcta cagccagctg
     1381 gaacattcgt ctcgaaggaa attgcttcat ccacaaatcc atctttcatg gcgtaaactt
     1441 tcccgctgat ggacccgtaa tgaaaaagaa gacaattgac tgggataagt ccttcgaaaa
     1501 aatgactgtg tctaaagagg tgctaagagg tgacgtgact atgtttctta tgctcgaagg
     1561 aggtggttct cacagatgcc aatttcactc cacttacaaa acagagaagc cggtcacact
     1621 gcccccgaat catgtcgtag aacatcaaat tgtgaggacc gaccttggcc aaagtgcaaa
     1681 aggctttaca gtcaagctgg aagcacatgc cgcggctcat gttaaccctt tgaaggttaa
     1741 aaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc
     1801 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1861 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1921 ata
//