##gff-version 3
##sequence-region BBa_K1124213 1 1923
BBa_K1124213	.	engineered_region	1031	1923	.	+	0	ID=BBa_K1124110;Name=BBa_K1124110
BBa_K1124213	.	ribosome_entry_site	1031	1042	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124110
BBa_K1124213	.	conserved_region	1035	1038	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124213	.	engineered_region	1049	1786	.	+	0	ID=BBa_K1124000;Name=BBa_K1124000;Parent=BBa_K1124110
BBa_K1124213	.	ribosome_entry_site	1049	1747	.	+	0	ID=annotation2338833;Name=amilGFP;Parent=BBa_K1124000
BBa_K1124213	.	stop_codon	1781	1783	.	+	0	ID=annotation2338831;Name=stop;Parent=BBa_K1124000
BBa_K1124213	.	tag	1748	1780	.	+	0	ID=annotation2338832;Name=LVA degradation tag;Parent=BBa_K1124000
BBa_K1124213	.	stop_codon	1784	1786	.	+	0	ID=annotation2338830;Name=stop;Parent=BBa_K1124000
BBa_K1124213	.	terminator	1795	1923	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124110
BBa_K1124213	.	terminator	1795	1874	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124213	.	stem_loop	1806	1849	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124213	.	engineered_region	1795	1874	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124213	.	terminator	1883	1923	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124213	.	stem_loop	1890	1909	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124213	.	stop_codon	1916	1916	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124213	.	engineered_region	1883	1923	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124213	.	polyA_site	1910	1923	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124213	.	promoter	988	1022	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K1124213	.	engineered_region	1	979	.	+	0	ID=BBa_K1124212;Name=BBa_K1124212
BBa_K1124213	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K1124212
BBa_K1124213	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1124213	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1124213	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1124213	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1124213	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1124213	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1124213	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1124212
BBa_K1124213	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1124213	.	terminator	851	979	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1124212
BBa_K1124213	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1124213	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1124213	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1124213	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1124213	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1124213	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1124213	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1124213	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1124213	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101;Parent=BBa_K1124212
>BBa_K1124213
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctacta
gagaaagaggagaaatactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaag
gcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaatt
agtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgt
tgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcat
ttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccat
ctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaa
atgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgcc
aatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggac
cgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggtt
aaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata