BBa_K1124213
type: ComponentDefinition
persistent identity: BBa K1124213
display id: BBa_K1124213
version: 1
was generated by: iGEM to SBOL conversion
description: Pconst (strong)-luxR-Pconst (strong)-amilGFP-LVA
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1124110
component: BBa_J23101
component: BBa_K1124212
sequence annotation: BBa_K1124212
sequence annotation: BBa_J23101
sequence annotation: BBa_K1124110
sequence: https://synbiohub.org/public/igem/BBa_K1124213_sequence/1

BBa_K1124110
type: Component
persistent identity: component2344239
display id: component2344239
version: 1
top level: BBa_K1124213
definition: BBa_K1124110
access: public

BBa_J23101
type: Component
persistent identity: component2344224
display id: component2344224
version: 1
top level: BBa_K1124213
definition: BBa_J23101
access: public

BBa_K1124212
type: Component
persistent identity: component2344223
display id: component2344223
version: 1
top level: BBa_K1124213
definition: BBa_K1124212
access: public

BBa_K1124212
type: SequenceAnnotation
persistent identity: annotation2344223
display id: annotation2344223
version: 1
top level: BBa_K1124213
location: https://synbiohub.org/public/igem/BBa_K1124213/annotation2344223/range2344223/1
component: BBa_K1124212

https://synbiohub.org/public/igem/BBa_K1124213/annotation2344223/range2344223/1
type: Range
persistent identity: range2344223
display id: range2344223
version: 1
top level: BBa_K1124213
direction: direction/reverse
start: 1
end: 979
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2344224
display id: annotation2344224
version: 1
top level: BBa_K1124213
location: https://synbiohub.org/public/igem/BBa_K1124213/annotation2344224/range2344224/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1124213/annotation2344224/range2344224/1
type: Range
persistent identity: range2344224
display id: range2344224
version: 1
top level: BBa_K1124213
direction: direction/reverse
start: 988
end: 1022
orientation: inline

BBa_K1124110
type: SequenceAnnotation
persistent identity: annotation2344239
display id: annotation2344239
version: 1
top level: BBa_K1124213
location: https://synbiohub.org/public/igem/BBa_K1124213/annotation2344239/range2344239/1
component: BBa_K1124110

https://synbiohub.org/public/igem/BBa_K1124213/annotation2344239/range2344239/1
type: Range
persistent identity: range2344239
display id: range2344239
version: 1
top level: BBa_K1124213
direction: direction/reverse
start: 1031
end: 1923
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K1124110
type: ComponentDefinition
persistent identity: BBa K1124110
display id: BBa_K1124110
version: 1
was generated by: iGEM to SBOL conversion
description: amilGFP(+LVA) generator(+RBS, +terminator)
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0015
component: BBa_K1124000
sequence annotation: BBa_B0034
sequence annotation: BBa_K1124000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124110_sequence/1

BBa_B0034
type: Component
persistent identity: component2341931
display id: component2341931
version: 1
top level: BBa_K1124110
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2341943
display id: component2341943
version: 1
top level: BBa_K1124110
definition: BBa_B0015
access: public

BBa_K1124000
type: Component
persistent identity: component2341936
display id: component2341936
version: 1
top level: BBa_K1124110
definition: amilGFP (+
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2341931
display id: annotation2341931
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341931/range2341931/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341931/range2341931/1
type: Range
persistent identity: range2341931
display id: range2341931
version: 1
top level: BBa_K1124110
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K1124000
type: SequenceAnnotation
persistent identity: annotation2341936
display id: annotation2341936
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341936/range2341936/1
component: BBa_K1124000

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341936/range2341936/1
type: Range
persistent identity: range2341936
display id: range2341936
version: 1
top level: BBa_K1124110
direction: direction/reverse
start: 19
end: 756
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2341943
display id: annotation2341943
version: 1
top level: BBa_K1124110
location: https://synbiohub.org/public/igem/BBa_K1124110/annotation2341943/range2341943/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124110/annotation2341943/range2341943/1
type: Range
persistent identity: range2341943
display id: range2341943
version: 1
top level: BBa_K1124110
direction: direction/reverse
start: 765
end: 893
orientation: inline

BBa_K1124212
type: ComponentDefinition
persistent identity: BBa K1124212
display id: BBa_K1124212
version: 1
was generated by: iGEM to SBOL conversion
description: Pconst (strong)-luxR
created: 2013-09-14T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: Coming soon!
owned by: james
owned by: myers
mutable description: Coming soon!
discontinued: false
dominant: false
group u list: _1436_
m user id: 0
owner id: 13160
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Coming soon!
star: false
creator: Takahiro Yamada
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_C0062
component: BBa_B0034
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1124212_sequence/1

BBa_B0015
type: Component
persistent identity: component2343774
display id: component2343774
version: 1
top level: BBa_K1124212
definition: BBa_B0015
access: public

BBa_C0062
type: Component
persistent identity: component2343764
display id: component2343764
version: 1
top level: BBa_K1124212
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component2343761
display id: component2343761
version: 1
top level: BBa_K1124212
definition: BBa_B0034
access: public

BBa_J23101
type: Component
persistent identity: component2343759
display id: component2343759
version: 1
top level: BBa_K1124212
definition: BBa_J23101
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2343759
display id: annotation2343759
version: 1
top level: BBa_K1124212
location: https://synbiohub.org/public/igem/BBa_K1124212/annotation2343759/range2343759/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K1124212/annotation2343759/range2343759/1
type: Range
persistent identity: range2343759
display id: range2343759
version: 1
top level: BBa_K1124212
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2343761
display id: annotation2343761
version: 1
top level: BBa_K1124212
location: https://synbiohub.org/public/igem/BBa_K1124212/annotation2343761/range2343761/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1124212/annotation2343761/range2343761/1
type: Range
persistent identity: range2343761
display id: range2343761
version: 1
top level: BBa_K1124212
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2343764
display id: annotation2343764
version: 1
top level: BBa_K1124212
location: https://synbiohub.org/public/igem/BBa_K1124212/annotation2343764/range2343764/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K1124212/annotation2343764/range2343764/1
type: Range
persistent identity: range2343764
display id: range2343764
version: 1
top level: BBa_K1124212
direction: direction/forward
start: 62
end: 817
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2343774
display id: annotation2343774
version: 1
top level: BBa_K1124212
location: https://synbiohub.org/public/igem/BBa_K1124212/annotation2343774/range2343774/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1124212/annotation2343774/range2343774/1
type: Range
persistent identity: range2343774
display id: range2343774
version: 1
top level: BBa_K1124212
direction: direction/reverse
start: 851
end: 979
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

amilGFP (+
type: ComponentDefinition
persistent identity: BBa K1124000
display id: BBa_K1124000
version: 1
was generated by: iGEM to SBOL conversion
description: amilGFP (+LVA), yellow reporter protein with degradation tag
created: 2013-09-10T11:00:00Z
modified: 2015-05-08T01:09:17Z
mutable provenance: PCR (template: BBa_K592010, primers shown on our wiki(UT-Tokyo 2013))
owned by: james
owned by: myers
mutable description: amilGFP (+LVA) yellow reporter protein with degradation tag. The strong yellow color is visible to the naked eye under natural room light both in a LB liquid medium and on a LB agar plate. You can easily visualize gene expression without special equipment. Rapid degradation of the protein allows real-time imaging . It is also useful as a fluorescent reporter. For detailed usage, see UT-Tokyo 2013 wiki. This is the LVA tagged version of BBa_K592010. LVA tag was added to increase decay rate by PCR using the primers shown on our wiki (UT-Tokyo 2013). amilGFP is a coral fluorescent protein from Acropora millepora. It is a member of the GFP family. Excitation maximum : 503 nm Emission maximum : 512 nm Quantum yield : 0.67 Molar Extinction : 75200 LVA tag is one of the ssrA degradation tag, which decreases the protein half-life when added to the C-terminus. References Andersen, J. B., Sternberg, C., Poulsen, L. K., Bj??rn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246. Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., & Matz, M. V. (2002). Diversity and evolution of the green fluorescent protein family. Proceedings of the National Academy of Sciences, 99(7), 4256-4261.
discontinued: false
dominant: false
experience: experience/None
group u list: _1436_
m user id: 0
owner id: 13134
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: LVA tag(11 aa) was added by PCR. As in the previous study(1). Additional 2 aa (Stu1 restriction site) was also added, which may not be necessary for unstabilization of the protein. RPAANDENYALVA double stop codon [1]Andersen, J. B., Sternberg, C., Poulsen, L. K., Bj??rn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.
star: false
creator: Yuta Otsuka
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: amilGFP
sequence annotation: LVA degradation tag
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K1124000_sequence/1

amilGFP
type: SequenceAnnotation
persistent identity: annotation2338833
display id: annotation2338833
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338833/range2338833/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338833/range2338833/1
type: Range
persistent identity: range2338833
display id: range2338833
version: 1
top level: amilGFP (+
direction: direction/forward
start: 1
end: 699
orientation: inline

LVA degradation tag
type: SequenceAnnotation
persistent identity: annotation2338832
display id: annotation2338832
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338832/range2338832/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338832/range2338832/1
type: Range
persistent identity: range2338832
display id: range2338832
version: 1
top level: amilGFP (+
direction: direction/forward
start: 700
end: 732
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2338831
display id: annotation2338831
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338831/range2338831/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338831/range2338831/1
type: Range
persistent identity: range2338831
display id: range2338831
version: 1
top level: amilGFP (+
direction: direction/forward
start: 733
end: 735
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2338830
display id: annotation2338830
version: 1
top level: amilGFP (+
location: https://synbiohub.org/public/igem/BBa_K1124000/annotation2338830/range2338830/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1124000/annotation2338830/range2338830/1
type: Range
persistent identity: range2338830
display id: range2338830
version: 1
top level: amilGFP (+
direction: direction/forward
start: 736
end: 738
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1124213_sequence/1
type: Sequence
persistent identity: BBa K1124213 sequence
display id: BBa_K1124213_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124110_sequence/1
type: Sequence
persistent identity: BBa K1124110 sequence
display id: BBa_K1124110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124212_sequence/1
type: Sequence
persistent identity: BBa K1124212 sequence
display id: BBa_K1124212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1124000_sequence/1
type: Sequence
persistent identity: BBa K1124000 sequence
display id: BBa_K1124000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtcttattcaaagcatggcatcgtacaagaaatgaagacgaaataccatatggaaggcagtgtcaatggccatgaatttacgatcgaaggtgtaggaactgggtacccttacgaagggaaacagatgtccgaattagtgatcatcaagcctgcgggaaaaccccttccattctcctttgacatactgtcatcagtctttcaatatggaaaccgttgcttcacaaagtacccggcagacatgcctgactatttcaagcaagcattcccagatggaatgtcatatgaaaggtcatttctatttgaggatggagcagttgctacagccagctggaacattcgtctcgaaggaaattgcttcatccacaaatccatctttcatggcgtaaactttcccgctgatggacccgtaatgaaaaagaagacaattgactgggataagtccttcgaaaaaatgactgtgtctaaagaggtgctaagaggtgacgtgactatgtttcttatgctcgaaggaggtggttctcacagatgccaatttcactccacttacaaaacagagaagccggtcacactgcccccgaatcatgtcgtagaacatcaaattgtgaggaccgaccttggccaaagtgcaaaaggctttacagtcaagctggaagcacatgccgcggctcatgttaaccctttgaaggttaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z